Production of self-organizing protein nanomaterials in endotoxin-free Escherichia coli (original) (raw)

AI-generated Abstract

Despite significant advancements in recombinant technologies, Escherichia coli remains highly utilized for the production of recombinant proteins. This research introduces an endotoxin-free E. coli strain, KPM335, achieved through deletions of multiple genes related to LPS biosynthesis, leading to the expression of recombinant proteins while minimizing endotoxic reactions. Further explorations demonstrated that this strain can successfully produce complex protein assemblies, such as functional inclusion bodies and tumor-targeted nanoparticles, showing comparable yields to wild-type strains while exhibiting differences in architecture, cellular uptake, and biodistribution in colorectal cancer models.