The morphology of redox-dye-treated HbH-containing red cells: differences between cells treated with brilliant cresyl blue, methylene blue and new methylene blue (original) (raw)
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Dyes and Stains: from molecular structure to histological application
Frontiers in Bioscience, 2014
In the present review, the chemistry of dyes as well as the interaction mechanisms between tissue and dye has been detailed, and also some of the key factors affecting the selectivity of dyes by certain cellular structures have been mentioned. Moreover, due to the relevance that histological stains have acquired in biomedical research, some of the most common stains have been described, pointing out previous and current applications in basic and applied research.
Indonesian Journal of Medical Laboratory Science and Technology
Tissue staining using hematoxylin-eosin (HE) is a standard method of histopathological staining. The tissue staining is hampered when there is no hematoxylin reagent in laboratory. Therefore, other reagents are needed that can replace the use of hematoxylin. Methylene blue is a basic dyes that interact with cell nuclei which has a negative ionic charge of the tissue. It can be used as an alternative nuclei staining. This study aims to evaluate the use of 1% of methylene blue in cell nuclei staining in histopathological preparations. The research sample were 15 pathology preparations which were randomly selected including breast cancer, cervical cancer and ovarian cancer in the bank of sampel at anatomical pathology laboratory of RSUD Dr. Slamet Garut, Indonesia. The experiment showed that the methylene blue dyes yielded “worth” result (40%) and “poorly” result (60%). Further research can be carried out by modifying the pH of 1% of methylene blue reagent so that it can maximize the s...
Reactive dyes for living cells: Applications, artefacts, and some comparisons with textile dyeing
Coloration Technology, 2021
An inclusive chemical definition of “reactive” dyeing of textiles is introduced, encompassing the CI Azoic, CI Mordant, CI Reactive, CI Sulphur and CI Vat dye application classes. Such reactive dyeing increases fibre retention of dye and makes application practically possible. The analogous application of dyes and fluorescent probes as microscopic stains in biology and medicine is outlined, focussing on using reactive fluorescent probes with living cells. Parallels with textile dyeing are noted, eg, enhanced probe retention and facilitation of probe application. However, the primary purpose of using reactive probes with live cells is detection of properties of biological systems: to identify biological structures and chemical/biochemical contents; assess biological functions and physicochemical properties; and determine changes in locations of cells and cell components. Problems occurring with such probes are outlined, particularly the problematic character of many standard protocol...
Effects of Monovalent Cations on Red Cell Shape and Size
Human erythrocytes were incubated in isotonic solutions of different monovalent cations. The apparent size of the red cells measured on scanning electron microscopic pictures decreases in the order Li + > Na § = K + > Rb +. These differences in size are abolished after pretreatment with trypsin, which removes a large part of the charges associated with membrane glycoproteins. Shape alterations are also observed. Normal biconcave shapes are visible after Na + or K + incubation, whereas Li § leads to flabby, flattened cells with a certain tendency to crenation, and Rb + causes more pronounced biconcavity with a certain tendency to cupping. The overall effects of pretreatment with trypsin are similar to those of Li § Our results provide evidence that the electrostatic repulsion of glycoproteins and other charged membrane components may play an essential role in maintaining red cell shape.
Rate factors in staining by Alcian Blue
The Histochemical Journal, 1974
Synopsis. Alcian Blue stains much more slowly than other basic dyes, and differential staining by the dye is greatly influenced by differences in the rate of staining of various basophilic components. Epithelial mucin stains most rapidly, followed by mast cell granules, pericapsular cartilage matrix, interstitial cartilage matrix, nuclei and cytoplasmic chromidial substance (presumably RNA), in that order. Sites containing nucleic acid stain intensely under some conditions, e.g. after some days in Alcian Blue at pH 4 and room temperature and, therefore, do not lack affinity for the dye. Nucleic acids are not, however, in general easily stained with Alcian Blue, being extremely slow-staining and also being easily leached from the section by hot or acidic solutions.