Direct and indirect impact of the bacterial strain Pseudomonas aeruginosa on the dissolution of synthetic Fe(III)- and Fe(II)-bearing basaltic glasses (original) (raw)
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Journal of Hazardous Materials, 2006
A basaltic glass and a vitrified bottom ash were incubated at 25 • C in a growth medium (based on casaminoacids) inoculated with Pseudomonas aeruginosa. Bacterial growth and mineral concentrations in different compartments (bacterial cells, growth medium and biofilm) were monitored in short-term (3 days), and long-term experiments involving repeated renewals of the culture medium during 174 days. In short-term experiments, while the concentration of iron increased in the presence of bacteria, a decrease in Ni and Zn was observed in the growth medium compared to the sterile condition. During long-term experiments, such differences gradually decreased and disappeared after 78 days. On the contrary, iron concentration remained higher in the biotic condition compared to the sterile one. Bacterial growth resulted within a few days in the formation of a biofilm, which lead to the cementation of the altered glass grains. Most of the constituents of the glass (Si, Mg, Fe, Ti, Ba, Co, Zn, Cu, Ni and Cr) were found in the biofilm, while the chemical composition of the bacterial cells was very different.
Frontiers in Microbiology
Phylogenetically and metabolically diverse bacterial communities have been found in association with submarine basaltic glass surfaces. The driving forces behind basalt colonization are for the most part unknown. It remains ambiguous if basalt provides ecological advantages beyond representing a substrate for surface colonization, such as supplying nutrients and/or energy. Pseudomonas stutzeri VS-10, a metabolically versatile bacterium isolated from Vailulu'u Seamount, was used as a model organism to investigate the physiological responses observed when biofilms are established on basaltic glasses. In Fe-limited heterotrophic media, P. stutzeri VS-10 exhibited elevated growth in the presence of basaltic glass. Diffusion chamber experiments demonstrated that physical attachment or contact of soluble metabolites such as siderophores with the basaltic glass plays a pivotal role in this process. Electrochemical data indicated that P. stutzeri VS-10 is able to use solid substrates (electrodes) as terminal electron donors and acceptors. Siderophore production and heterotrophic Fe(II) oxidation are discussed as potential mechanisms enhancing growth of P. stutzeri VS-10 on glass surfaces. In correlation with that we discuss the possibility that metabolic versatility could represent a common and beneficial physiological trait in marine microbial communities being subject to oligotrophic and rapidly changing deep-sea conditions.
Microbial interactions with silicate glasses
npj Materials Degradation, 2021
Glass alteration in the presence of microorganisms has been a topic of research for over 150 years. Researchers from a variety of disciplines, including material science, biology, chemistry, geology, physics, and cultural heritage materials preservation have conducted experiments in this area to try and understand when, how, and why microorganism may interact and subsequently influence the alteration of glass. The breadth and depth of these studies are the topic of this review. This review presents a detailed history and a comprehensive overview of this field of research, while maintaining focus on the terrestrial alteration of anthropogenic silicate glasses. Within this manuscript is a schema for bio-interaction with silicate glasses and an outline of an evidence-based hypothesis on how these interactions may influence glass alteration processes. Topics discussed include microbial colonization of glass, development, and interactions of biofilms with glass surface, abiotic vs. bioti...
Biologically mediated dissolution of glass
Chemical Geology, 1995
Microbes play an important role in the dissolution of natural and synthetic glasses. The frequent technical use of glass and the abundance of glass at the earth's surface make this process one of the most important weathering reactions. In particular the alteration of volcanic glass provides a direct pathway for mantle-derived materials into the hydrosphere. To begin understanding the kinetics of these processes, we carried out three experiments exposing to seawater polished surfaces of nuclear-waste glass. The durations of experiments were: 410 days with continuously flowing sand-filtered, unsterilized seawater (approx. 2 X lo6 1; Exp. 1) , 126 days with a marine cyanobacterium culture (50 ml, Exp. 2)) and for 225 days under sterile conditions (250 ml, Exp. 3). The sterile experiment (Exp. 3) did not show significant signs of alteration. Exp. 1 resulted in development of a several Frn thick biofilm and surface corrosion with grooves exceeding 10 km in length and 0.5 pm in width. Cyanobacterial cultures also developed a biofilm on the glass surfaces, thinner than in Exp. 1. The glass surfaces themselves were corroded with approximately OS-pm.-sized pits clustering in 5 pm broad zones parallel to the polishing direction.
Textural and chemical effects of bacterial activity on basaltic glass: an experimental approach
Chemical Geology, 1995
Naturally altered basaltic glass may show features such as pitted trxturcs and variable degree of element mobilization relative to the fresh parent. The alteration process has generally been considered from only a chemical/ physical point of view, but recent observations of bacteria in altered glass have, however, led IO questions about the importance of microbial activity. In order to examine this, an experiment has been performed in which basaltic glass samples were immersed in growth media at room temperature for up to 394 days, inoculated with bacteria derived from a naturally altered pyroclastic deposit (Surtsey tuff). During the experiment it was observed that bacteria had a great affinity for attachment to the glass surface, which is in most cases connected to the production ofextracellular polymers. Further, different species of bacteria were dominant at different time intervals. The bacteria activity caused a general decrease in p from 8.5 to 5.8 during the time of the experiment. After 46 days of incubation, SEM studies of samples show rare examples of clear etching marks on the surface corresponding in size and shape of a minor group of bacteria. A local corrosion in a more irregular manner was observed after 1 S 1 days. Chemical analysts of the glass surface show no difference in composition compar bd to the fresh glass ai this stage, i.e. any dissolution is congruent. Bacteria and biofilms attached to the glass surface show accumulation of elements, of which Al and Si could only have been derived by dissolution of the glass. However, the extent of accumulation of various elements may differ pronouncedly within and between the runs at 44, 77 and 181 days. This scatter probably reflects the diversity of the community and the ability of the different species of bacteria to accumulate elements. After 394 days the outermost glass rim, 1 Corn in thickness, is highly depleted in all cations, except Si, which is relatively enriched. This incongruent dissolution of the glass, has only been active durir'g the last 7 months of the experiment. The alteration rate is increased, at least, by a factor of 10 compared to that of the first 6 months. This is thought to be caused by the activity of a new, dominant bacterium group uuring this period. Microanalyses of the bacteria attached to the residual, leached glass rim, show more frequent accumulation tif Si. and generally their chemistries are more homogenous than that observed in the other, shorter-termed runs. Bacterial activity may hence have a great influence on the textural and chemical developments commonly observed in naturally altered basaltic glass deposits.
Antibacterial effects and dissolution behavior of six bioactive glasses
Dissolution behavior of six bioactive glasses was correlated with the antibacterial effects of the same glasses against sixteen clinically important bacterial species. Powdered glasses (<45 lm) were immersed in simulated body fluid (SBF) for 48 h. The pH in the solution inside the glass powder was measured in situ with a microelectrode. After 2, 4, 27, and 48 h, the pH and concentration of ions after removing the particles and mixing the SBF were measured with a normal glass pH electrode and ICP-OES. The bacteria were cultured in broth with the glass powder for up to 4 days, after which the viability of the bacteria was determined. The antibacterial effect of the glasses increased with increasing pH and concentration of alkali ions and thus with increased dissolution tendency of the glasses, but it also depended on the bacterium type.
npj Materials Degradation, 2023
This work aims to investigate the role played by a model Mn-oxidizing bacterial strain and its exudates on the alteration of Mn-bearing potash-lime silicate glasses representative of medieval stained glass windows. Two model glasses, with or without manganese, were prepared and used for abiotic and Pseudomonas putida inoculated dissolution experiments. Results show that the presence of P. putida slows down the dissolution kinetics while changing the dissolution stoichiometry. In biotic experiments, the acidification of the solution at the beginning of the experiment favors the release of K. After a few days, a drop in Mn and P in solution is observed, retained by bacterial cells. Reciprocally, the amount of glass influences bacterial behavior. The more glass, the faster the bacterial population increases in size and produces siderophore. In the presence of the Mn-bearing glass, siderophore production is followed by the formation of brown phases, identified as Mn oxides.
The Role of Biofilm on the Alteration of Glasses: Example of Basaltic and Nuclear Glasses
2006
It is generally accepted that alterations of rocks and anthropogenic products are not exclusively driven by the interaction with water or mineral aqueous solutions. Organic compounds as well as microorganisms are important in mineral degradation processes, together with secondary mineralization. However, the exact role of biofilms in these processes remains unclear. In our study we tested two materials, a tholeiitic basaltic glass and the reference French nuclear glass SON68 17 LIDC2A2Z1. Experiments were carried out for 19 weeks using a modified soxhlet's device at 25°C. We developed a specific growth medium which allows both the growth of Pseudomonas bacterium and a precise measurement, using ICP-MS, of trace elements solubilized from the two glass materials. The thickness of biofilms, analyzed by confocal laser microscopy was 40mum for both materials. These biofilms are able to efficiently trap most of the glass constituents, some of them being potentially toxic. They also f...
International Journal of Food Microbiology, 2008
Application of antimicrobial chemicals is a general procedure in the cleaning and disinfection of food-contacting surfaces. Adhesion to glass surfaces and chemically induced detachment of Pseudomonas fluorescens ATCC 13525 T were studied in situ, under flow conditions, in a wellcontrolled parallel plate flow chamber (PPFC). Ortho-phthalaldehyde (OPA) and cetyltrimethyl ammonium bromide (CTAB) were applied separately, at several concentrations, to attached bacteria and their subsequent detachment was monitored. Following treatments the remaining adhered bacteria were characterized in terms of viability and cell size. Simultaneously, the planktonic cell surface was characterized in order to correlate PPFC results with thermodynamic approaches for adhesion evaluation, and surface free energy of chemically treated cells with adhesion strength. About 2.8 × 10 6 cells/cm 2 adhered to the glass surface after 30 min of bacterial flow, although thermodynamic analyses evidenced unfavourable adhesion. The independent application of OPA and CTAB promoted bacterial detachment to a small extent (16% of total cells). The remaining adhering bacteria were totally non-viable for OPA ≥ 0.75 mM and CTAB ≥ 0.25 mM, showing a lack of correlation between bacterial viability and detachment. The cellular size decreased as attachment proceeded and with chemical treatment. Both chemicals altered the cell surface properties, increasing the cell-glass adhesion strength, and promoting the emergence of polar characteristics. The overall results emphasize that OPA and CTAB were markedly ineffective in removing glass-attached P. fluorescens, demonstrating that bacteria can be non-viable but remain strongly attached to the adhesion surface.