Antibacterial Activity of Flax Seeds Extract Rinse against Streptococcus mutans Colonies (original) (raw)
Related papers
2018
Introduction: Flaxseed from the flax plant (Linum usitatissimum), which has been cultivated for domestic use since prehistoric times. This study aims to investigate presence of antibacterial effect of flaxseed extract against selected oral pathogen in-vitro. Materials and Methods: This is a laboratory experimental in vitro study using selected oral pathogens cultured in nutrient agar. The pathogens then were inoculated in nutrient based broth and incubation for 24hours. Flaxseed extract efficacy was tested by measurement of the zone of inhibition. The antibacterial activities were compared with streptomycin as positive control and DMSO as negative control. The statistical analysis was done by using SPSS. Results: There is positive antibacterial effects of flaxseed extract against Streptococcus mutans, Streptococcus Pyogenes, Pseudomona Aerigenosa and was comparable in efficacy to that of Streptomycin. Conclusion: This study concludes that flaxseed extract has antibacterial activity ...
Bactericidal activities of GM flax seedcake extract on pathogenic bacteria clinical strains
2014
Background: The antibiotic resistance of pathogenic microorganisms is a worldwide problem. Each year several million people across the world acquire infections with bacteria that are antibiotic-resistant, which is costly in terms of human health. New antibiotics are extremely needed to overcome the current resistance problem. Results: Transgenic flax plants overproducing compounds from phenylpropanoid pathway accumulate phenolic derivatives of potential antioxidative, and thus, antimicrobial activity. Alkali hydrolyzed seedcake extract containing coumaric acid, ferulic acid, caffeic acid, and lignan in high quantities was used as an assayed against pathogenic bacteria (commonly used model organisms and clinical strains). It was shown that the extract components had antibacterial activity, which might be useful as a prophylactic against bacterial infection. Bacteria topoisomerase II (gyrase) inhibition and genomic DNA disintegration are suggested to be the main reason for rendering antibacterial action.
Antimicrobial Efficacy of Chemical and Herbal Agents against Streptococcus mutans: An in vitro study
Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2018
Objective: To compare and evaluate antimicrobial efficacy of chlorhexidine gluconate and fennel seeds against Streptococcus mutans. Material and Methods: Three agar petri dishes for Streptococcus mutans were prepared. The methanolic extract of fennel seed and 0.2% chlorhexidine were inoculated on the each petri dish and incubated at 37ºC for 24 hours. Zone of growth inhibition for each extract was measured in millimeters using antibiotic inhibiting zone measuring scale. Data was analyzed using SPSS statistical software. The comparison of inhibition zone against S. mutans using CHX and fennel seed extract was done using Mann-Whitney and Wilcoxon tests. All statistical analyses were set at a significance level of p<0.05. Results: The mean of zone of inhibition of S. mutans after inoculation with 0.2% chlorhexidine and fennel seed extract are 21.44 ±1.46, 15.34 ±0.74 respectively. There was statistically significant difference in the mean of inhibition zone between 0.2% chlorhexidine and fennel seed against S. mutans (p=0.047). Conclusion: Chlorhexidine and fennel seed are effective in inhibiting the growth of cariogenic bacteria. Therefore, both herbal and chemical agents can be effectively used to reduce pathogenic oral microflora.
Open Access Macedonian Journal of Medical Sciences
AIM: This study investigated the antibacterial efficacy of five plant extracts, as well as the combinations of the two most effective plant, extracts either with or without commercial varnish (MI varnish) on the in vitro growth of Streptococcus mutans and Lactobacillus acidophilus in comparison to MI varnish using agar disk diffusion and broth dilution methods. METHODS: Methanolic extractions of five plants (Cinnamon, Turmeric, Ginger, Clove and Black seed,) were tested against the growth of the two oral pathogens. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the two most effective extracts, and their combinations with different ratios were evaluated against the growth of the two oral pathogens, followed by incorporating the two effective plants or each into commercial MI varnish to be assessed against the oral pathogens in comparison to MI varnish. RESULTS: Only Cinnamon and Clove produced inhibition zones against Strep...
Potential antibacterial effects of flaxseed and Nigella sativa extracts on Streptococcus pyogenes
IIUM Journal of Orofacial and Health Sciences
Antibiotic resistance is a major global problem, associated with inadvertent drug usage. Herbal interventions are a therapeutic strategy that warrants greater research attention. Flaxseed and Nigella sativa are well recognized original super foods that have demonstrated potent anti-microbial and anti-biofilm activities. In the oral cavity, the bacterial population is a result of the dynamic relationship between pathogens and commensals Streptococcus pyogenes is an important global human Gram-positive pathogen that causes a wide variety of acute infections, it is highly virulent since it has the ability overcome the host defence system. This in vitro study aims to evaluate antimicrobial activity of flaxseed and Nigella sativa extract against S. pyogenes. Ethanolic extract of flaxseed and Nigella sativa extracts were prepared and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against S. pyogenes was estimated. The results of this study show tha...
International journal of clinical pediatric dentistry
Streptococcus mutans has been implicated as primary microorganisms which cause dental caries in humans. There has been an increased interest in the therapeutic properties of some medicinal plants and natural compounds which have demonstrated antibacterial activities. Grape is one of the plants of this group which contains tannin and polyphenolic compound. To evaluate and compare antibacterial activity of grape seed extract at different concentrations with chlorhexidine gluconate against S. mutans. Grape seeds were extracted with ethanol/water ratio of 70:30 volume/volume. The extracts were filtered through Whatman No. 1 filter paper until it becomes colorless. Streptococcus mutans strains were taken. To check the antimicrobial properties of grape seed extract at different concentration and chlorhexidine gluconate, they were added to S. mutans strain and incubated for 48 hours than colony-forming units/mL were checked. Grape seed extract at higher concentration were found to be more ...
Fibres and Textiles in Eastern Europe, 2016
Flax fibres are known as favourable to human skin, and linen clothing positively influences some physiological parameters, guaranteeing optimal comfort for users. The advantages of linen are not only related to ensuring proper breathability for human skin and to a lack of allergic activity, but also to flax fibre antibacterial properties, whose are not precisely described in available literature. This paper describes the examination of flax fibres extracted from Polish flax varieties: Artemida, Modran, Sara, Nike and Luna in terms of their antibacterial activity. The fibres were extracted from stems with the application of two different methods: dew and water retting. The extraction method had an influence on the chemical composition of the fibres, resulting in different levels of fibre antibacterial properties. The biological activity of the flax fibres was estimated using clinical strains of Staphylococcus aureus. The flax fibres extracted by the dew retting method from the varieties MODRAN, SARA and NIKE showed the highest amount of lignin content in their chemical composition and the strongest antibacterial activity in comparison to other fibres tested.
Oral diseases are major health problems with dental caries and periodontal diseases among the most important preventable global infectious diseases. Increasing of refractory periodontal diseases is due to non-sensitivity of cariogenic bacteria to antibiotics, and the lack of new alternatives will be threat to danger imminent. This serious increase in the non-sensitivity of microbes to antibiotics may be opened to search for alternative approaches for exploring of new drugs with specific therapeutic activities. In this study cariogenic bacterium Streptococcus mutans strain ATCC 25175 was used as a test organism for evaluation the efficacy of water extracts of eight different medicinal plants as antibacterial and anti-biofilm agents. The best percentage of inhibition of these extracts against S. mutans strain ATCC 25175 was recorded as; Pomegranate peelà Cloveà Coffeeeà green tea. The cytotoxicity of the most potent extracts has been measured against fibroblast cells. Generally, the green tea extract was the safer extract followed by coffee, pomegranate peel and clove extracts. The recorded IC 50 of the all used plant extracts on fibroblast cells ranged from 10-1.25%. with exception for green tea extract that didn't show IC 50 even with the maximum used concentration. The potency of the three selected extracts to to inhibt the biofilm formation by the tested strain could be summarized by the following order: pomegranate peelà coffeeà clove. Oral health influences the general quality of life and poor oral health is linked to chronic conditions and systemic diseases. Finally, give a recommendation for using some of these extracts to restrict the bacterial growth and reduction of the prevalence of periodontitis and cariogenic diseases.
EFFECT OF BREADFRUIT LEAF EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS BIOFILM IN VITRO
International Journal of Applied Pharmaceutics, 2019
Breadfruit leaf has potent antibacterial properties that could be used to reduce biofilms in the oral cavity. The purpose of this study was to analyze the antibacterial effect of the breadfruit leaf extract on the growth of Streptococcus mutans in vitro. Methods: S. mutans ATCC 25175 was cultured in a 96-well plate and incubated at 37°C for 20 h (accumulation phase) and 24 h (maturation phase). The breadfruit leaf extract was added at the following concentrations: 5%, 10%, 15%, 20%, 40%, 80%, and 100%. The viability of S. mutans was tested with the MTT assay at a wavelength of 490 nm. The results were analyzed by one-way analysis of variance. Results: In the accumulation phase, a significant decrease was found in S. mutans viability at different concentrations of the breadfruit leaf extract, but in the maturation phase, a significant decrease was found in the S. mutans viability at the 5% concentration. The other groups decreased significantly compared with the control group (*p<0.05). The viability of S. mutans after adding the breadfruit leaf extract at all concentrations was lower in the accumulation phase than that in the maturation phase. Conclusion: In the accumulation phase, breadfruit leaf extract at concentrations of 5%, 10%, 20%, 40%, 80%, and 100% can reduce S. mutans biofilm viability.
Use of Traditional Indian Plants in the Inhibition of Caries-Causing Bacteria -Streptococcus mutans
The aim of the study was to comparatively evaluate the antibacterial activity of six Indian plant extracts and 0.2% chlorhexidine against clinical strains of Streptococcus mutans, which were isolated from the plaque samples of 45 pediatric patients. Six plant extracts were prepared in three different forms, namely aqueous extracts, organic solvent-based extracts and crude (raw) extracts. The antimicrobial sensitivity testing was done by agar well diffusion method. Antimicrobial activity of the extracts was determined by measuring the mean zones of inhibition (mm) produced against the bacterial isolates. Results showed that crude garlic extract exhibited greater antibacterial activity than chlorhexidine. Aqueous extract of amla and organic solvent-based extract of ginger showed the maximum antibacterial activity against S. mutans, whereas aqueous extract of tulsi and organic solvent based extract of amla showed the minimum antibacterial activity. This study suggests that plant extracts like garlic in crude form, amla as aqueous infusion and ginger as alcoholic tincture have potential for the control of S. mutans. These extracts can be used as an alternative remedy for dental caries prevention or in the form of mouthwash, which is safe and economical.