Efficacy of endodontic applications of ozone and low-temperature atmospheric pressure plasma on root canals infected with Enterococcus faecalis (original) (raw)
Related papers
This study aimed to compare the antimicrobial efficacy of low-temperature atmospheric pressure plasma (LTAPP) design and gaseous ozone delivery system with 25% NaOCl on Enterococcus faecalis in root canal walls and dentine tubules. The samples were divided into LTAPP (n=12), ozone (n=12), NaOCl (positive control,n=12) and saline (negative control,n=6) groups. Microbial samples were collected using paper points and dentin chips from root canals. Antimicrobial efficacy was assessed by counting the colonyforming units of Ent. faecalisbefore and after each irrigation protocol. Data were analysed using Kruskal–Wallis, Wilcoxon signed-rank, Friedman and Bonferroni t (Dunn’s test)-tests (P=005). The microbial sampling with paper points showed antibacterial efficacy of NaOCl, LTAPP, ozone and saline in descending order, respectively (P<005). The microbial sampling with dentin chips demonstrated a superior efficacy of LTAPP compared with NaOCl in the middle third (P<005), while both had similar effects in coronal and apical thirds (P>005). NaOCl and LTAPP were better than ozone at the coronal and middle parts of the root canals (P<005). These findings led us to suggest that LTAPP, which has no thermal and chemical effects, may be of great aid in endodontic treatment.
Effectiveness of Ozone against E.Faecalis in a Root Canal Suspension -An In-Vitro Study.
IOSR Journals , 2019
Aim: To assess the antimicrobial efficacy of aqueous and gaseous ozone as an alternative antiseptic against endodontic pathogen Enterococcus faecalis in suspension. Methodology: Enterococcus faecalis, were grown in Mc conkey media in planctonic phenotype. Cultures were exposed to ozone gas, aqueous ozone & sodium hypochlorite (NaOCl; 0.5%), (control) for 1 min and the colony forming units were determined before and after exposure. Statistical analysis used: Data were analyzed by one way ANOVA with Tamhane post hoc tests to compare independent samples (ά=0.05). Results: Concentrations of gaseous ozone for I min/53ug almost and aqueous ozone 1min/20ug completely eliminated the suspended microorganisms as did 0.5% NaOCl. Conclusion: High-concentrated gaseous and aqueous ozone was dose-, strain-and time-dependently effective against the tested microorganisms in suspension.
Brazilian dental journal, 2013
This ex vivo study evaluated the antibacterial effect of intracanal medications in root canals contaminated with Enterococcus faecalis. Fifty single-rooted human teeth were contaminated with E. faecalis (ATCC 29212) and incubated at 37°C for 21 days. The specimens were randomly divided into 5 groups according to the intracanal medication used: OZ-PG: ozonized propylene glycol; CH/CPMC: calcium hydroxide/camphorated paramonochlorophenol; OZ-PG/CH ozonized PG/CH; PC: positive control group (no medication); and NC: negative control group (no contamination). The samples were collected after 7 days (post-medication) and 14 days (final). Bacterial growth was checked by counting the colony-forming units (CFU). OZ-PG and CH/CPMC reduced significantly the CFU counts compared with PC in the post-medication and final samples, with no statistically significant differences among them. On the other hand, OZ-PG/CH did not reduce significantly the number of bacteria compared with PC. In conclusion,...
Efficacy of ozone on microorganisms in the tooth root canal
Collegium antropologicum, 2013
The aim of this study was to examine the effect of ozone gas on the remaining bacteria after chemomechanical instrumentation of tooth root canal. The study was carried out at the Department of Endodontics and Restorative dentistry, School of Dental Medicine, University of Zagreb. A total of 37 tooth root canals from 23 teeth (10 incisors, 2 canines, 8 premolars and 3 molars) with a diagnosis of chronic apical periodontitis (17 untreated teeth and 6 retreatments) from 20 adult patients (11 females and 9 male) were selected. Endodontic samples consisted of 74 swabs from 37 canals. The first root canal swab was taken following a completed chemomechanical instrumentation by a sterile paper point after rinsing the root canal with a sterile saline solution. The canal was dried and treated with ozone gas for 40 seconds (HealOzone, Kavo, Germany). After the ozone treatment the canal was rinsed with a sterile saline solution a second swab was taken. The swabs were stored in transport media u...
Nigerian Journal of Clinical Practice, 2014
Aim: To evaluate the potential antibacterial effect of recurrent doses of topical gaseous ozone on the Enterococcus faecalis biofilms growth in human root canals in vitro. Materials and Methods: One hundred and thirty four human single-rooted mandibular premolars were enlarged to a size 35 K-File. Each root canal were inoculated with an overnight culture of Enterococcus faecalis ATCC 29212 in tryptic soy broth for 24 hours and incubated for 7 days at 37°C. At 7-day interval, 4 specimens were prepared for Scanning Electron Microscope (SEM) analysis to confirm the presence and purity of biofilms whilst the other contaminated root canals were irrigated and disinfected. One hundred root canals of total 134 specimens were selected to create the experimental groups and divided into 5 subgroups. In each experimental group (n = 20) root canals), recurrent ozone doses were applied with different irrigation and disinfection protocols in 5 different time intervals. Bacterial growth was analyzed by counting viable E. faecalis on tryptic soy agar plates. Results: According to intergroup comparison results observed in the final sample collection analysis, the amount of remaining bacteria in the positive control group were found to be significantly higher compared to Groups 1, 2, 3, 4, 5 and the material control group (P < 0.01). The remaining amount of bacteria in the last count of Group 1 were found to be significantly higher compared to Group 2 (P < 0.05), Group 4 (P < 0.01), Group 5 (P < 0.05) and the material control group (P < 0.01). Conclusion: The application of topical gaseous ozone in recurrent doses provides a positive effect in the removal of E. faecalis biofilm from root canals. However, during disinfection procedure, the combined use of recurrent doses of topical gaseous ozone with 2% NaOCl enhanced its antibacterial effect against E. faecalis biofilm.
Evidence-Based Efficacy of Ozone for Root Canal Irrigation
Journal of Esthetic and Restorative Dentistry, 2008
CA, USA) using ultrasonics. I also bubble ozone gas (HealOzone, KaVo, Biberach, Germany) into this ozonated water and use ozonated oil (Lime Technologies Ltd., Capetown, South Africa) as a medicament. COMPARISON OF THE USE OF OZONE AND SODIUM HYPOCHLORITE IN ROOT CANAL THERAPY Oxygen has a dramatically toxic effect to microaerophilic and anaerobic bacteria. Virtej and colleagues22 compared the antimicrobial performance of four systems used as root canal irrigants. Seventy instrumented and initially sterile roots with open access cavities and containing a paper point were carried by one volunteer in the oral cavity for 1 week. After removal, the samples were taken for microbiologic analysis. The root canals were then disinfected with the Endox Endodontic System (Lysis S.r.l., Nova Milanese [MI], Italy), MTAD (Dentsply Tulsa Dental, Tulsa, OK, USA), 3% sodium hypochlorite (NaOCl), or HealOzone, and thereafter, the samples were repeated for microbiologic analysis. The roots were then sealed and incubated for a further week, after which bacterial growth was again determined. After disinfection, there was a significant decrease in the absolute bacterial count between each disinfection method and the positive control group. There was no statistically significant difference between the 3% NaOCl, MTAD, and HealOzone groups. The Endox device showed the least antibacterial effect with significant differences to MTAD and HealOzone. Bacterial regrowth after 1 week of incubation was detected in all specimens of the control group, whereas the test groups showed several bacteria-free specimens. The authors concluded that ozone has great potential in endodontic antimicrobial use and that MTAD and HealOzone seem to be as effective as 3% NaOCl in reducing mixed bacterial infection in the root canal system.22 I would speculate that the antimicrobial effect of the ozone would have been even greater if it had been used as I recommended above. I personally feel that conventional irrigation (including NaOCl) should be used during cleaning and shaping, and ozonated water (ideally with ozone gas) should be used as the final irrigant with ultrasonication. Cardoso and colleagues2 concluded that the ozonated water, used as an irrigant agent, significantly reduced the number of Candida albicans and Enterococcus faecalis in root canals in human teeth. A review23 identified four studies6,24-26 investigating the bactericidal effect of ozone as compared with 2.5 to 5% sodium hypochlorite as irrigation solutions in endodontics. Nagayoshi and colleagues6 found nearly the same antimicrobial activity (against E. faecalis and Streptococcus mutans) and a lower level of cytotoxicity of ozonated water as compared with 2.5% NaOCl. They stated, "Ozone is known to act as a strong antimicrobial agent against bacteria, fungi, and viruses. In the present study, we examined the effect of ozonated water against Enterococcus faecalis and Streptococcus mutans infections in vitro in bovine dentin. After irrigation with ozonated water, the viability of E. faecalis and S. mutans invading dentinal tubules significantly decreased. Notably, when the specimen was irrigated with sonication, ozonated water had nearly the same antimicrobial activity as 2.5% sodium hypochlorite (NaOCl). We also compared the cytotoxicity against L-929 mouse fibroblasts between ozonated water and NaOCl. The metabolic activity of fibroblasts was high when the cells were treated with ozonated water, whereas that of fibroblasts significantly decreased when the cells were treated with 2.5% NaOCl. These results suggest that ozonated water application may be useful for endodontic therapy."6 ASK THE EXPERTS 288
The Effect of Gaseous Ozone in Infected Root Canal
Open access Macedonian journal of medical sciences, 2018
During the treatment of chronic apical periodontitis and pulp necrosis the main role is to irrigate the root canal. The aim of this in vivo study was to irrigate with 0.9% NaCl (Natrium Chloride), 2.5 % NaOCl (Sodium Hypochlorite Solution, Sigma Aldrich - Germany) and 2% CHX (Chlorhexidine Digluconate Solution, Sigma Aldrich - Spain) combined with Gaseous Ozone (Prozone WH, Austria). This study was realised in the University Dentistry Clinical Centre of Kosovo (UDCCK), respectively in the Department of Endodontic and Dental Pathology, Dental Branch, Faculty of Medicine, Prishtina, Kosovo. The 40 subjects involved in this study belonged to both genders, in age between 15 -65 years. The sample selection was randomised. The retroalveolar radiography for each patient was taken in the suspected tooth. As a therapeutic plan the authors decided to disinfect the root canal with the irrigants, as follows: 2.5 % NaOCl, 2 % CHX and gaseous ozone. The statistical analyses were based on Kruskal ...
Effectiveness of ozone against endodontopathogenic microorganisms in a root canal biofilm model
International Endodontic Journal, 2009
Huth KC, Quirling M, Maier S, Kamereck K, AlKhayer M, Paschos E, Welsch U, Miethke T, Brand K, Hickel R. Effectiveness of ozone against endodontopathogenic microorganisms in a root canal biofilm model. International Endodontic Journal, 42, 3-13, 2009.