Correction to: Evaluation of the effect of polyethylene glycol (PEG) on germination and morphological characteristics of bread wheat (original) (raw)
Related papers
Early Growth Behaviour of Wheat Genotypes as Affected by Polyethylene Glycol (PEG-6000)
Pakistan journal of scientific and industrial research, 2016
Polyethylene glycol (PEG-6000) is often used for the early establishment screening of the wheat genotypes against drought stress conditions. A collection of twenty-one newly developed bread wheat genotypes (developed through conventional and mutational breeding techniques) and four commercial drought tolerant check varieties were included in screening at seedling stage under three treatments of PEG-6000; T1 (0.5 MPa) and T2 (0.75 MPa) along with control T3 (distill water only). Three important early growth establishment traits like germination percentage, root length and shoot length of the wheat genotypes were observed. Significant variation among genotypic means regarding the observed traits was recorded at both the treatments. Wheat genotypes performance including check varieties for germination percentages was recorded as T1 (86.1%), T2 (72.4%) with reduction percentage of 12.5% and 33.8%. Root length of genotypes decreased by 37.0% in 0.5 MPa and 82.75% in 0.75 MPa, whereas, shoot length reduction was recorded as 38.9% (T1) and 84.6% (T2) as compared to control. This study provided essential information about the performance of advanced wheat genotypes under water stress conditions at early seedling establishment.
Germination Behavior of Wheat (Triticum Aestivum L.) as Influenced by Polyethylene Glycol (PEG
Pre-sowing seed treated with Polyethylene Glycol (PEG) helps to enhance the germination behavior of seed. So, a lab experiment was conducted to find out the effect of various Polyethylene Glycol (PEG) concentrations on the germination behavior of wheat. Wheat seeds of BARI Gom 27 and BARI Gom 28 were pre-soaked in 0, 5, 10, 15 and 20% PEG solution and untreated seeds were served as control. Results revealed that seed priming enhanced germination percentage (GP), vigor index (VI) and germination index (GI) of wheat seed. The highest GP (95.55%), VI (201.00) and GI (43.73) were obtained from seeds of BARI Gom 27 pre-treated with 10% PEG solution compared to BARI Gom 28 (75.55%, 128.71 and 27.12 of GP, VI and GI, respectively) and then decreased gradually with increasing PEG concentration. Therefore, seed priming helps to enhance the germination behavior of wheat seed.
Wheat Germination and Seedlings Under Peg-Induced Conditons
2018
Climate changes are causing frequent drought which leads to shortage of water, reducing wheat production worldwide. The three winter wheat genotypes were examined for their response to drought at the germination and seedling stage. Research was carried out under controlled conditions where polyethylene glycol (PEG 6000) was used in 20% concentration to create artificial drought stress. The results revealed that genotype ’Vulkan’ showed maximum germination percentage (85%) in control while the minimum germination percentage was observed in ‘Žitarka’ (43%) under PEG treatment. At control level seed germination percentage was the higher in all three genotypes compared to drought treatment. ‘Vulkan’ had the highest shoot length in control plants (14.18 cm) which was significantly reduced in PEG treatment (6.93 cm). Maximum fresh weight of shoots was recorded in ‘Vulkan’ (1.53 g) followed by ‘Antonija’ (1.23 g) in control treatment. Under control conditions, genotype ‘Antonija’ showed ma...
Effect of Polyethylene GLYCOL-6000 on Wheat (Triticum Aestivum L.) Seed Germination
2013
The response of twenty-five wheat (Triticum aestivum L.) varieties to different water stress levels were studied in a series of experiments conducted in the laboratory on Polyethylene glycol-6000. The experiments on germination with twenty-five wheat varieties were conducted in a growth cabinet maintained at 30/25 0 C day/night temperature with 16 hours photoperiod. Seeds were germinated in glass tray lined with filter paper moistened with polyethylene glycol (PEG-6000) solutions of 0.0,-0.25,-0.50,-0.75 and-1.0 MPa osmotic potential. Germination percentage decreased with decrease in water potential of the medium. The water uptake reached at a peak value within 48 hours in all the genotypes under all the water stress conditions. The varieties Sarsabz and Kiran-95 showed comparatively better performance of germination under water stress than other wheat varieties.
Effect of Genotyps and Polyethylene Glycol on Immature Embryo Culture in Wheat
Journal of Agricultural Chemistry and Biotechnology, 2018
Seven wheat genotypes were used in this investigation to study the effect of genotypes, levels of polyethylene glycol (PEG) concentrations and their interaction on immature embryo induction, as well as, estimating heritability for in vitro traits. The genotypes were in vitro evaluated for immature embryos culture ability at four levels of PEG concentrations. Significant differences between genotypes were obtained for some traits at four PEG levels. Levels of mean squares were significant for some traits such as number of green point, plant length and number of tillers. Whereas, mean squares of interaction between genotypes × PEG levels was insignificant for all traits except number of tillers. The results demonstrated that the greatest percentages for green points and plant weight were recorded by the hybrid Sakha 93 × Gemmiza 9. In addition, Sakha 93 × Sids 1 was the best genotype for root length and number of tillers. Whereas, the greatest value for number of plantlets was observed in Sakha 93 and sakha 94. The addition of 10 g/l PEG to MS nutrient medium and 1 mg/l NAA and 0.5 mg/l Kinetin in regeneration medium could be the best concentration for immature embryo purpose in wheat. Furthermore, the genetic variations were highly positive for some traits such as number of green point at levels A and B, number of plants at levels A,B and D, plant weight at level A, plant length at level C, root length at level A and number of tillers at levels A,B and D. The values of heritability were ranged between 53.66 to 90.48% for number of tillers at levels D and A, respectively. The results indicated that the genes controlled growing traits in wheat were highly affected by media composition.
2023
Wheat productivity has been steadily declining over the past few years, and abiotic stresses are responsible for more than half of all yield losses. Stress from drought is one of the most significant factors that can impede plant growth and production wherever in the world. The main goal of this study was to examine how low water potential, which was caused by polyethylene glycol (PEG 6000), on the germination and biochemical parameters of Hungarian wheat landraces. Seven Hungarian wheat landraces varieties (Szentesi; Nyirádi; Kiszombori; Háromfai; Tapiószelei; Nagykállói, and Szajlai) were tested for germination characteristics; seedling parameters; water relative content; tolerance index; and enzyme activities in the presence of different five concentrations of water stress (0; 5; 10; 15 and 20%) of polyethylene glycol (PEG 6000). At the Research Institute of Nyiregyhaza in Hungary, a laboratory experiment using Factorial Experiment in Randomized Completely Design (RCD) was carried out in four replicates. According to the data, the Háromfai landrace outperformed all other landraces under research and recorded the highest values of all analyzed features, followed by Tapiószelei; Kiszombori; Szajlai; Nyirádi; Szentesi and Nagykállói landraces varieties. All characteristics under study were significantly decreased as water stress concentrations (PEG 6000) were increased from 0 up to 20%. In comparison to other wheat landraces, Háromfai and Tapiószelei landraces varieties had the highest water relative content (WRC), tolerance index (TI), and α as well β-amylase amylase activities, and were able to induce better drought tolerance. These results suggest that these landraces could be a good resource for breeding programs and cultivation under drought stress conditions. Furthermore, the results of correlation analysis did not neither positive nor negative correlation between the Hungarian wheat landraces varieties and all of analyzed traits. On contrary, the correlation analysis results show negative correlation between the drought stress levels and studied traits.
Plant Breeding and Biotechnology
Wheat is globally an important cereal crop. Environmental stress, especially drought stress can play an important role in the reduction of plant growth, specifically during germination in arid and semi-arid regions. Polyethylene glycol (PEG) treated hydroponic conditions create negative osmotic potential which is compared with moisture deficit stress. The main objective of this study was to investigate the effects of PEG 6000 induced moderate osmotic stress on germination indices of 22 wheat varieties. In order to study the effects of osmotic stress on germination indices in wheat cultivars, an experiment was conducted, using a completely randomized design with three replications under two different levels of PEG-6000: 0% and 10%. PEG stress significantly reduced percent germination, shoot length and root length. PEG stress significantly increased root-shoot ratio and oven dry weight. Principal component analysis revealed response of traits of tolerant wheat varieties under osmotic stress. Correlation study revealed the significant relationships among germination indices. The variety BARI Gom-30 recorded comparatively higher root length (6 cm), shoot length (7.8 cm), root-shoot ratio (1.37) followed by the variety Sonalika whereas the variety Kalaysona recorded the lowest root length (2.7 cm) and shoot length (2.8 cm) under PEG stress. Evolved information of this research including selected traits such as germination percentage, root-shoot ratio and dry weight of seedlings could be exploited in wheat breeding program for drought and osmotic stress tolerance.
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2015
This study was conducted to determine the effects of some priming treatments on seed germination properties, grain yield, and several agromorphological characteristics of bread wheat. Two commonly grown bread wheat varieties, namely Adana-99 and Pandas, were selected for experimentation conducted during the 2007-08 and 2008-09 growing periods. The seeds of the Adana-99 and Pandas wheat varieties were primed with the following: (1) distilled water, (2) 100 ppm indole-3-acetic acid (IAA), (3) 2.5% potassium chloride (KCl), (4) 1% potassium dihydrogen phosphate (KH 2 PO 4), (5) 10% polyethylene glycol (PEG-6000), or (6) gibberellic acid (GA 3 , used only for field experiments). Nonprimed seeds were used as the control group. First 1000 mL of priming media was prepared for each priming treatment, and seeds of both varieties were rinsed in the solution for 12 h at room temperature. Germination percentage at two different temperatures (10 °C and 20 °C), coleoptile length, seedling emergence percentage, and seedling growth rate were evaluated under laboratory conditions. Primed seeds of both varieties were sown on two different dates under field conditions to evaluate certain agromorphological characteristics. PEG, IAA, and distilled water treatments increased seed germination percentage, seedling emergence percentage, and seedling growth rate. PEG, KCl, and hydropriming treatments increased grain yield compared to the control. Among the different priming agents used in the study, PEG, KCl, and hydropriming were the most effective treatments to attain higher germination percentage and grain yield.
Bangladesh Agronomy Journal, 2023
Germination characteristics, early seedling growth and seed metabolic efficiency of four wheat genotypes (BARI Gom 28, BARI Gom 29, BAW 1177 and ESWYT 29) were evaluated under 15% polyethylene glycol (PEG-6000) induced osmotic stress (-3 bar). Germination characteristics (germination rate, coefficient of germination and germination vigor index), shoot and root length, shoot and root dry weight, and seed metabolic efficiency decreased under osmotic stress induced by PEG than control treatment. But the degree of reduction was different for various wheat genotypes. Genotype BAW 1177 showed the highest performance in respect to germination and early seedling traits at both control and PEG induced osmotic stress than other three genotypes. So, genotype BAW 1177 can be considered as relatively drought tolerant genotype.