Epidemiology and Associated Risk Factors for Brucellosis in Small Ruminants Kept at Institutional Livestock Farms in Punjab, Pakistan (original) (raw)
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Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan
The Journal of Infection in Developing Countries, 2015
Introduction: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. Methodology: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR). Results: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk sampl...
Revisiting Brucellosis in Small Ruminants of Western Border Areas in Pakistan
Pathogens
Brucellosis, globally known bacterial zoonosis, is endemic to Pakistan. B. abortus in bovines, B. melitensis in small ruminants and B. canis in dogs mainly cause this disease. A total of 1821 sera (1196 from sheep and 625 from goats) from animal herds near the Pakistan–Afghanistan border were collected. In parallel testing of sera for anti-Brucella antibodies (B. abortus and B. melitensis) was carried out by RBPT and indirect ELISA. The presence of Brucella DNA in sera was tested by real-time PCR. The overall percentage of seropositive samples was 0.99 (18/1821) by both tests. All positive samples originated from Baluchistan territory which translated into 1.76% (18/1021). None of the positive sera had signals for Brucella DNA and none of sera from goats carried detectable antibodies. Both tests showed an almost perfect agreement with Kappa statistics. The flock size was found to be associated with the presence of anti-Brucella antibodies. The samples of Khyber Pakhtunkhwa (KPK) tes...
Seroprevalence of Brucellosis in Sheep and Humans in District Kohat, Pakistan
Advances in Animal and Veterinary Sciences, 2014
| Brucellosis is a bacterial disease and is caused by genus Brucella. It is highly prevalent zoonotic disease in developing countries including Pakistan. This study was conducted to determine the prevalence of brucellosis in sheep and humans of district Kohat, Khyber Pakhtunkhwa. For this purpose, one hundred blood samples from each humans and sheep (50 each from both sexes) were randomly collected at three different tehsils (Lachi, Seni Gumbat and Kohat) of district Kohat. The serum samples were tested for presence of anti-Brucella antibodies by Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT). Moreover, a total of 50 milk samples were also collected from various sheep herds for subsequent conduction of milk ring test (MRT). The estimated prevalence of ovine brucellosis was 12.12%, 09% and 08.82% in Lachi, Seni Gumbat and Kohat, respectively, with an overall prevalence of 10% in the district. Whereas, SAT and RBPT demonstrated the prevalence as 7% and 6%, respectively, in humans. The results of current study validated that brucellosis was widespread in district Kohat with a relatively higher prevalence being recorded in ewes and occupationally exposed women than their male counterparts. Furthermore, the prevalence of brucellosis was significantly higher in sheep and humans of Lachi and Seni Gumbat tehsils as compared to Kohat. Results of the current study may support the health care organizers to formulate suitable control plans against Brucellosis.
Seroprevalence of Brucellosis in human, cattle, and buffalo populations in Pakistan
2008
Bovine brucellosis, a disease of major economic and public health importance, is a worldwide problem (1). The disease is predominantly an occupational illness in farm and livestock workers, veterinarians, slaughterhouse employees, meat inspectors, and laboratory personnel (2). Individuals consuming dairy products in areas of endemic infection and those that handle animals and animal carcasses are at high risk of contracting brucellosis (3,4). A survey conducted in Pakistan found that 6.79% of humans were positive for Brucella abortus antibodies (5). In different areas of Pakistan the prevalence of B. abortus in bovines ranged between 3.25% (6) and 4.4% (7). A study conducted by Buchanan et al. (8) revealed that brucellosis is an abattoir-associated disease and slaughterhouse workers have the greatest risk of contracting the disease. The prevalence of brucellosis has been reported to be between 3.2% (9) and 3.4% (10) in humans, and 6.47% in cattle and sheep in Turkey (9). Chen et al. (11) used RBPT and SAT for the detection of B. abortus antibodies in cows in the U.S., but these tests are not practical when performed on a large number of samples for various infectious diseases, including brucellosis. ELISA can be used as a diagnostic test for the screening of antibodies, as it is reported to have a sensitivity of 95%-100% (12). Güllüce and Leloğlu (13) used ELISA and the milk ring test (MRT) for the detection of B. abortus antibodies in dairy cows in Kars, Turkey. Abuharfeil and Abo-Shehada (14) recommended RBPT and ELISA for mass screening of brucellosis in sheep in Jordan. Ganesan and Anuradha (15) used RBPT and Dot-ELISA for mass screening of brucellosis in bovines in India. In Pakistan most studies on brucellosis were conducted on organized government livestock and private livestock farms (16), and, to some extent, in humans. Little is known about the prevalence of B. abortus in Pakistan at abattoirs and among humans that work with livestock. The present study investigated the prevalence of B. abortus in cattle at abattoirs, farms, and villages, as well as in humans in direct contact with livestock.
Sero-incidence of brucellosis in small ruminants of semiarid Rajasthan
The Indian journal of animal sciences
The present study was taken up to ascertain the prevalence of brucellosis in sheep and goats of semiarid region of Rajasthan by Rose Bengal Plate Test and commercially available ELISA kit. RBPT detected brucella antibodies in 94 (8.82%) out of 1065 and ELISA in 118 (11.47%) out of 472 sheep serum samples tested. The highest seroprevalence was recorded in Tonk (24.47% and 43.29%), followed by Ajmer district (11.16% and 14.06%) by RBPT and ELISA, respectively. RBPT detected Brucella antibodies in 12 (1.65%) out of 723 and ELISA detected 19 (4.67%) out of 406 goat serum samples. The incidence in Tonk district was 1.31% and 10.44%, in Ajmer 2.20% and 2.63%, in Bundi 1.75% and 1.85%, and in Bhilwara 3.4% and 5.47%, by RBPT and ELISA, respectively. The combined incidence in sheep and goats of semiarid Rajasthan was 5.92% by RBPT and 15.6% by ELISA. Our results showed higher seroprevalences in small ruminants of Tonk, Bhilwara and Ajmer districts. There is a need to screen human population dealing with husbandry, health and marketing of small ruminants and to develop effective control and prevention strategies for sheep and goats of this area against brucellosis.
Seroprevalence of brucellosis in sheep and goats in the Arabian Gulf region
Veterinary World, 2020
Background and Aim: Brucellosis is a zoonosis that occurs worldwide. There were more efforts to control brucellosis in all countries. This study was performed to determine the seroprevalence of brucellosis in sheep and goats in some areas in the Arabian Gulf. Materials and Methods: The study analyzed 8500 sera from non-vaccinated sheep and goats. Animals included 6441 sheep (3420 from farms and 3021 from quarantine) and 2059 goats (1580 from farms and 479 from quarantine). Sera were tested using the Rose Bengal Plate Test (RBPT) and confirmed with an indirect enzyme-linked immunosorbent assay (i-ELISA) test. Final confirmation analyzed blood samples from confirmed infected animals (n=30, 23 sheep and seven goats) using polymerase chain reaction (PCR) and culture. Results: The serological examination showed that 62/8500 of animals (0.729%, confidence interval [CI] 95% 0.57-0.94) were seropositive for brucellosis. Thirteen of 5000 (0.26%, CI 95% 0.15-0.45) and 49/3500 (1.4%, CI 95% 1.1-1.8) of animals from farms and quarantine were seropositive, respectively. Out of the 6441, 46 (0.71%) sheep and 16/2059 (0.78%) goats were seropositive. i-ELISA confirmed 41/62 RBPT-positive animals – 41/8500 (0.482%, CI 95% 0.36-0.65). Eight of 5000 of these animals (0.16%, CI 95% 0.08-0.32) and 33/3500 (0.94%, CI 95% 0.67-1.3) were confirmed positive in farms and quarantine, respectively. Thirty of 6441 (0.466%) and 11/2059 (0.534%) cases were positive in sheep and goats, respectively. PCR confirmed 18 of 41 positive animals (0.212% of all sera, CI 95% 0.13-0.34) identified by both RBPT and i-ELISA. Three of 5000 (0.06%, CI 95% 0.019-0.19) and 15/3500 (0.429%, CI 95% 0.26-0.71) from farms and quarantine were confirmed positive. Tissue samples (uterine, supra-mammary, testicular, and accessory glands lymph node) were collected from positive animals, as detected by RBPT and i-ELISA, at culling or slaughtering. Using in vitro culture, 14/30 were confirmed positive – 3/7 from farms (two sheep and one goat) and 11/23 from quarantine (nine sheep and two goats). Biovar 1 was dominant. PCR confirmed 23/30 tissue samples, 4/7 from farms (three sheep and one goat), and 19/23 from quarantine (15 sheep and four goats). Conclusion: The overall brucellosis rate in sheep and goats is 0.48%, with fewer animals from farms testing positive (0.16%) in this area of the Arabian Gulf. The infection appears to be well controlled, and continuous effort is still needed to maintain control and completely eradicate brucellosis. Additional support is needed for testing and slaughterhouse monitoring. In quarantine (imported animals), brucellosis infection in the slaughterhouse (0.94%) could pose a risk for transmission and spread of infection. The effort is needed to monitor this threat, and PCR is a sensitive and time-saving test for brucellosis diagnosis. All 14 confirmed positive samples were Biovar 1 dominant.
OALib, 2015
Aim: Brucellosis, as a zoonotic disease, is characterized by reduced fertility in animals. This study has aimed to assess the prevalence of brucellosis in sheep and cows rearing in Kermanshah Province. Methodology and Results: The study was conducted in the regions of Kermanshah whereas people had seropositive results for brucellosis. Serum samples of sheep and cow flocks were screened using tow conventional methods. The sheep was the most ruminant infected with brucella. The most seropositive cases were found in the year 2011. Conclusion, Significance and Impact of Study: Control and eradication programs among animals should be regarded as a priority measures in prevention of human brucellosis. The zoonotic aspects of brucellosis must be considered. When the disease exists in animals, especially in ovine and caprine, it is a concern for human public health. In order to control this zoonotic disease, close cooperation of health and veterinary organizations is necessary.
Veterinary World, 2021
Background and Aim: Brucellosis is a zoonotic disease of high economic and public health importance in large and small ruminant populations worldwide. A cross-sectional study was conducted to determine the seroprevalence and risk factors of brucellosis in small ruminants in organized farms in the southern region of India. Materials and Methods: Farms exclusively rearing sheep and goats were selected based on the number of animals (small, medium, or large) and the location of the farm (urban, periurban, or rural). A total of 1499 serum samples; 1001 from sheeps and 498 from goats were sourced from six sheep and four goat farms and tested using Rose Bengal Plate and indirect Enzyme-Linked Immunosorbent Assay tests. Results: The apparent prevalence of brucellosis was higher in sheep (8.29%, 95% CI 6.7-10.1) than goats (5.82%, 95% CI 4.0-8.2). The true adjusted population level seroprevalence was also higher in sheep, at 7.7% (95% CI 6.0-9.6) than in goats, at 5.1% (95% CI 3.2-7.6). According to bivariate categorical analysis, six highly significant (p<0.001) animal- and farm-level risk factors for sheep were age, breed, number of lambings, history of abortion, rural farms, and presence of dogs on the farm. In goats, five significant risk factors were found: History of abortion, separate sheds, dogs on the farm, weekly veterinary consultation, and lack of brucellosis awareness. In a logistic regression model, abortion (OR adjusted 10.8, 95% CI 1.2-96.12), rural farms (OR adjusted 8.5, 95% CI 3.6-20.0), and absence of separate sheds on the farms (OR 1.9, 95% CI 1.1- 3.5) were found to be significant risk factors for ovine brucellosis. Conclusion: The use of complementary measures to tackle the multiple animal- and farm-level risk factors may help to reduce the disease burden in the absence of a vaccination policy for small ruminants in India.
African Journal of Microbiology Research, 2012
Brucellosis causes a great economic loss to the livestock industries through abortion, infertility, birth of weak and dead offspring, increased calving interval and reduction of milk yield and it is endemic in Bangladesh. The present study was performed to know the seroprevalence of brucellosis for 1000 ruminants (135 Buffaloes, 465 cattle, 230 goats and 170 sheep) in five different districts of Bangladesh by four conventional serological tests such as: Rose Bengal Plate Test (RBT), tube agglutination test (TAT), competitive enzyme-linked immunosorbent assay (C-ELISA), and Fluorescent polarization assay (FPA). Sheep has the highest prevalence (8.24%) of brucellosis. The seroprevalence of brucellosis was significantly higher in animals with previous abortion record in case of buffaloes, cattle, goats and sheep than that with no abortion record. C-ELISA can be the most suitable choice for extensive use in many kinds of livestocks and accurate estimation of Brucella antibodies in ruminants in Bangladesh.
Veterinary World, 2018
Aim: The present study aimed to study the seroprevalence of brucellosis in small ruminants of Gujarat state, India, using Rose Bengal Plate test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). Materials and Methods: A total of 2444 sera samples (675 sheep and 1769 goat) from unorganized sector and 1310 sera samples (861 sheep and 449 goat) from seven organized farms were collected for brucellosis screening. Results: In unorganized sector, 23.70% sheep (160/675) and 15.99% goat (283/1769) were positive by RBPT and 24.44% sheep (165/675) and 17.24% goat (305/1769) by iELISA. The organized sector samples showed higher seroprevalence in goat (7.79 %, 35/449) than sheep (4.06 %, 35/861) by RBPT. Similarly, in iELISA, goat samples showed a higher seroprevalence (9.35%, 42/449) compared to sheep (7.50%, 65/861). The diagnostic sensitivity and specificity of RBPT with ELISA were 88.69% and 99.65%, respectively, and showed a significant difference (p≤0.0001). The Chi-square analysis revealed a significant difference in seroprevalence between sectors (p≤0.01) and species (p≤0.01). Conclusion: The seroprevalence of brucellosis in small ruminants of Gujarat was investigated and showed a higher prevalence of brucellosis and warrants the implementation of proper preventive measures.