Preparation of Silver Nanoparticles from Herbal Plant (original) (raw)
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Metallic nanoparticles are being used in each period of science alongside building counting medicinal and many other fields that fascinate the researchers to investigate new measurements for their individual worth which is by and large credited to their comparing little sizes. Among a few metal nanoparticles, silver nanoparticles have accomplished a unique core interest. Routinely silver nanoparticles are integrated by synthetic strategy utilizing synthetic compounds as lessening specialists which later wind up responsible for different natural dangers because of their general poisonous quality; inciting the genuine worry to create condition agreeable procedures. Therefore, to settle the goal; natural approaches are applied to fulfill the requirement; for example, green amalgamations utilizing natural particles gotten from plant sources as concentrates showing prevalence over synthetic as well as organic strategies. These plants based natural particles experience very controlled gathering for making them appropriate for the metal nanoparticle amalgamations. The present audit investigates the big variety of plant to be used towards fast and single step convention preliminary strategy with green standards over the traditional ones and depicts the antimicrobial exercises of silver nanoparticles.
Synthesis of silver nanoparticles from plant extracts and their antimicrobial application
International Journal of Biosciences (IJB)
Development of reliable and eco-friendly process for synthesis of nanoparticles is an important step in the field the of nanotechnology. Nanotechnology involves the tailoring of materials at the atomic level to attain unique properties, which can be suitably manipulated for the desired applications. Among them silver nanoparticles draw attention due to its unique physical, chemical and biological properties. Green principle route of synthesizing have emerged as alternative to overcome the limitation of conventional methods among which plant and microorganisms are majorly exploited. Employing plants towards synthesis of nanoparticles are emerging as advantageous compared to microbes with the presence of broad variability of bio molecules in plants can act as capping and reducing agents and thus increases the rate of reduction and stabilization of nanoparticles. This review focuses on the green synthesis of silver nanoparticles using various plant sources and its applications in cancer treatment. Generally surgery, chemotherapy and radiation treatment are the most prevalent therapeutic option for cancer. Unfortunately these treatments have various side effects due to lack of targeted delivery and cancer specificity. To overcome these limitations, nanoparticle could ensure targeted drug therapy having very little side effects. In this review focus is given to silver nanoparticle, synthesized from natural plant extracts, as it is cost effective, eco-friendly, stable and safe in cancer treatment.
2020
Department of Chemistry, Kalinga University, Naya Raipur-492 101, Chhattisgarh, India<br> Department of Chemistry, Rungta College of Engineering & Technology, Bhilai-490 024, Chhattisgarh, India<br> Department of Chemical Engineering, National Institute of Technology Raipur, Raipur-492 010, Chhattisgarh, India<br> E-mail: dr.manisha.agrawal@rungta.ac.in<br> Manuscript received online 30 March 2020, accepted 01 June 2020 Silver nanoparticles (AgNPs) are gaining attention because of its wide application in medical science as antimicrobial and<br> antibacterial properties. A conventional method for synthesis of AgNPs consumes hazardous chemicals and during production<br> of NPs harmful by-products may also form. Proposed review suggests "greener technology" for synthesis of silver<br> nanoparticles with the help of easily available plants. Aqueous extract of leaf or roots of Impatiens balsamina, Lantana camara,<br> Eriobotrya ja...
Green Synthesis of Silver Nanoparticles using Azadirachta Indica and Ocimum Sanctum Leaf Extract
Current Science, 2019
In the present study, green synthesis of stable silver nanoparticles was done using methanolic leaf extract of Azadirachta indica L. as well as Ocimum sanctum L. The antimicrobial activity was screened against UTI causing bacteria, viz. Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus using disc diffusion method. Silver nanoparticles derived from tulsi leaf extract inhibited E. coli by 12.00 ± 0.65 mm, K. pneumoniae by 9.66 ± 0.11 mm and S. aureus by 23.00 ± 0.81 mm at concentrations 1 mg/ml. Similarly, silver nanoparticles derived from neem leaf extract inhibited K. pneumoniae by 9.11 ± 0.81 mm and S. aureus by 09.0 ± 1.17 mm at concentrations 1 mg/ml respectively. Lower concentration (1 mg/ml) of tulsi and higher concentration (4 mg/ml) of neem leaf methanolic extract was observed to be effective against urinary tract infections in human causative agent S. aureus.
IET Nanobiotechnology, 2018
Nanoparticles fabricated using medicinal plant extract has great potential in the area of nanomedicine. High surface to volume ratio of nanoparticle enhances local active biomolecules concentration, leading to many fold increase in the medicinal potentials. The silver nanoparticles (AgNP) fabricated using indigenous medicinal plants of India, Azadirachta indica and Syzygium cumini, have shown significant effect on the viability of prokaryotic and eukaryotic cells. Biofabrication of AgNP was confirmed using different spectroscopic and microscopic techniques. Extraction and purification of AgNP from nonconjugated plant moieties are done using centrifugation and size exclusion chromatography (SEC). The cytotoxic propensity of AgNP formulations were screened against Gram-positive (Bacillus subtilis), Gram-negative (Escherichia coli) bacteria, cancerous (HT1080) and non-cancerous (HEK293) cell lines. The nanoparticle formulations showed relatively higher cytotoxic propensity against Gram-positive bacteria and cancerous cell lines. Additionally, the surface roughness and reactive oxygen species (ROS) measurements indicated that AgNP formulations mediate the cell activity predominantly by ROS-mediated disruptive change in membrane morphology upon direct interaction with membrane. Hence, the nanoparticle formulations show enhanced selective cytotoxic propensity towards Gram-positive bacteria and cancerous cell lines.
Bioresources and Bioprocessing, 2014
Background In recent years, green synthesis of silver nanoparticles (AgNPs) has gained much interest from chemists and researchers. In this concern, Indian flora has yet to divulge innumerable sources of cost-effective non-hazardous reducing and stabilizing compounds utilized in preparing AgNPs. This study investigates an efficient and sustainable route of AgNP preparation from 1 mM aqueous AgNO3 using leaf extracts of three plants, Musa balbisiana (banana), Azadirachta indica (neem) and Ocimum tenuiflorum (black tulsi), well adorned for their wide availability and medicinal property. Methods AgNPs were prepared by the reaction of 1 mM silver nitrate and 5% leaf extract of each type of plant separately. the AgNPs were duely characterized and tested for their antibacterial activity and toxicity. Results The AgNPs were characterized by UV-visible (vis) spectrophotometer, particle size analyzer (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and energy...
Shanlax International Journal of Arts, Science and Humanities, 2020
Nanoparticles (NPs) are being widely used in different fields; therefore, there is growing interest in the development of a biological and environmental safety method for their production. Now a day’s chemical and physical methods are being used for the development of nanoparticle that is costly, time-consuming, and harmful for the environment. Plant-mediated synthesis of nanoparticles is a “Green chemistry” approach that connects different types of plants with nanotechnology. It has gained much more attention as a reliable, sustainable, and eco-friendly method for synthesizing a wide range of materials/nanomaterials. Plants are called nature’s “chemical factories” therefore, plants and plant extracts are the best options to produce different types of nanoparticles. In this present study, silver nanoparticles were synthesized by using leaf extract of Ocimum sanctum and aqueous silver nitrate solution through a simple and eco-friendly method. Then the developed silver nanoparticles w...
Antibacterial and Cytotoxic Potential of Biosynthesized Silver Nanoparticles by Some Plant Extracts
Nanomaterials
The provision of nanoparticles using biogenic material as a part of green chemistry is an attractive nanotechnology. The current research aimed to test the antimicrobial and cytotoxic efficacy of silver nanoparticles synthesized by extracts of Phoenix dactylifera, Ferula asafetida, and Acacia nilotica as reductant and stabilizing agents in silver nanoparticle formation. Synthesized nanoparticles were evaluated for their antimicrobial activity against Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa and Escherichia coli (Gram-negative) using an agar well diffusion assay. Furthermore, cytotoxic ability was investigated against LoVo cells. The potential phyto-constituents of plant extracts were identified by Fourier-transform infrared spectroscopy (FT-IR) techniques. Field emission scanning electron microscopy (FE-SEM), transmission electron microscope (TEM), and zeta potential analyzed the size and morphology of the biogenic nanoparticles. The current study revealed the ability of the tested plant extract to convert silver ions to silver nanoparticles with an average size that ranged between 67.8 ± 0.3 and 155.7 ± 1.5 nm in diameter. Biogenic AgNPs showed significant antibacterial ability (10 to 32 mm diameter) and anticancer ability against a LoVo cell with IC50 ranged between 35.15-56.73 µg/mL. The innovation of the present study is that the green synthesis of NPs, which is simple and cost effective, provides stable nano-materials and can be an alternative for the large-scale synthesis of silver nanoparticles.
2021
Nanotechnology is the modern research field which deals with design, synthesis and manipulation of particles structure range from 1-100nm in dimension. A nanoparticle is ultra-fine unit with dimension less than 100 nm (1 nm=10-9 meter).Because of their small size they have unique characteristics and their applications in various field such as medicines, engineering and environmental remediation. Nanoparticles can be prepared by various aapproaches like physical, chemical and biological .The biological approach is the emerging approach because it is easier than other methods. In this green synthesis aqueous solution of extract of Azadirathcha indica and AgNo3 were taken for study. The nanoparticles were prepared by using plant extract and silver metal ions. The nanoparticles were characterized by different spectral methods like UV, IR, DLS, SEM, XRD and Zeta analysis.
Biomedicine
Recently, silver nanoparticles (AgNPs) hold a centre stage in the vast arena of research in nanomedicine. Biological methods of synthesis of nanoparticles are being adopted to avoid the use of chemicals that are toxic and hazardous and used in chemical synthetic methods. The choice of the plant extracts used for the synthesis of AgNPs is based on their phytochemicals and pharmaceutical properties. Another importance of the phytosynthesis method is that it is simple, easy, fast, reliable and cost effective. The phytochemical components of the herbal extracts not only cause reduction of Ag+1 to Ag0, but also increase the biological activities of the nanoparticles. This article briefly describes the research on green synthesis of AgNPs using Indian medicinal plant extracts. We aim to provide a systemic depth of information regarding the effect of various reaction factors during the synthesis of AgNPs such as the concentration of silver salt and phytoextract, light intensity, time, temp...