ISOLATION OF FOUR STEROIDS FROM THE LEAVES OF FERN Adiantum latifolium Lam (original) (raw)
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Phytochemical and biological studies of Adiantum capillus-veneris L
Saudi Pharmaceutical Journal, 2011
Chromatographic fractionation of the alcoholic extract of the dried fronds of Adiantum capillus-veneris L. (Adiantaceae) yielded seven compounds: four triterpenoidal compounds belonging to adiantane and filicane groups were isolated from the hexane fraction and identified as isoadiantone (1); isoadiantol-B (2); 3-methoxy-4-hydroxyfilicane (3) and 3,4-dihydroxyfilicane (4) and three flavonoids were isolated from the ethyl acetate fraction and identified as: quercetin (5), quercetin-3-O-glucoside (6) and quercetin-3-O-rutinoside (rutin) (7). The identification of the isolated compounds has been established through their physical, chemical and spectroscopic methods including IR, 1 H NMR, 13 C NMR, HSQC, HMBC, NOESY and MS. Biological studies of the total alcoholic extract, hexane fraction and some of the isolated compounds showed an anti-inflammatory activity while the hypoglycemic study of the total alcoholic extract showed a significant activity.
Gas Chromatography and Mass Spectroscopy Analysis of Bioactive Compounds of Adiantum Latifolium Lam
International Research Journal Of Pharmacy, 2018
Adiantum latifolium Lam. is an important medicinal fern belongs to the family Adiantaceae. The medicinal property of Adiantum latifolium is yet to explore except a few secondary metabolites on quantitative analysis level. The present study is the maiden effort and aims at analyzing Gas Chromatography and Mass Spectroscopy analysis of whole plant Ethanolic extract of Adiantum latifolium Lam. The study was carried out with GC-MS instrument of ITQ 900 Model of Thermo Fisher Scientific make. The Ethanolic plants samples revealed fifteen bioactive compounds, through GC
Tropical Journal of Pharmaceutical Research, 2011
Adiantum is a genus of ca. 200 species in the family Adiantaceae, distributed extensively across the world from cool temperate zones to hot tropical regions. A lot of Adiantum species have been used in traditional Chinese medicine to cure human and animal diseases including relief of internal heat or fever, enhancement of urination, removal of urinary calculus, and sundry other curative claims. Chemical studies have shown the presence of various classes of compounds, the main ones being triterpenoids, flavonoids, phenyl propanoids, steroids, alicyclic acids, lipids and long-chain compounds. The extract of this genus as well as pure compounds isolated from it have been demonstrated to possess multiple pharmacological activities such as analgesic, antinociceptive, anti-implantation, and antimicrobial activities. In this review, we have addressed the phytochemistry and pharmacological activities of the Adiantum species in order to collate existing information on this plant as well as highlight its multi-activity properties as a medicinal agent.
2005
The water extracts and extracted phenols from gametophytes and different parts of sporophytes of the two ferns, Adiantum capillus-veneris L. and Adiantum lunulatum Burm. f., used as folkloric medicines in India, China, Tibet, America, Philippines and Italy, were investigated for their antifungal activity against Aspergillus niger and Rhizopus stolonifer. Both crude extracts and extracted phenols of gametophytes and different parts of sporophytes of both fern species were found to be bioactive against the fungal strains. Antifungal activity was found to be higher in gametophytes than different parts of sporophytes. Among the different parts of sporophyte, immature pinnule possesses highest fungistatic property. Adiantum capillus-veneris was found a better antifungal agent than Adiantum lunulatum.
Frontiers in Chemistry, 2017
Adiantum capillus-veneris is important endangered fern species with several medicinal properties. In this study, the leaves samples were extracted and separated using reversed phase HPLC with DAD for carotenoids, chlorophylls and phenolic compounds. Separation of carotenoids and chlorophylls were carried out using a tertiary gradient system of water, MTBE and methanol-water, while a binary gradient system of methanol-water-acetic acid was used for phenolic profiling. Results revealed eight carotenoids, four pheophytins, and two chlorophylls. Lutein (806.0 µg/g), chlorophyll b ′ (410.0 µg/g), chlorophyll a (162.4 µg/g), 9 ′-Z-neoxanthin (142.8 µg/g) and all-E-violaxanthin (82.2 µg/g) were present in higher amounts. The relatively high amounts of lutein may be one of the key indicator of beneficial antioxidant properties. The phenolic profile revealed a total of 13 compounds, namely 4-hydroxybenzoic acid, chlorogenic acid, caftaric acid, kaempferol glycosides, p-coumaric acid, rosmarinic acid, 5-caffeoylquinic acid, and quercetin glycosides. Kaempferol-3-sophorotrioside (58.7 mg/g), chlorogenic acid (28.5 mg/g), 5-O-caffeoylquinic acid (18.7 mg/g), coumaric acid (11.2 mg/g), and its derivative (33.1 mg/g) were present in high amounts. These results suggest that the reversed phase HPLC profiling of Adiantum leaves provides a better understanding in to the actual composition of bioactive compounds, which may be responsible for the potential medicinal properties. Adiantum leaves rich in important bioactive phytochemicals can be used as a possible source of nutraceuticals or as a functional food ingredient.
International journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: Phytosterols are group of steroidal alcohol play important roles in structural component in the cell membrane and play a role in membrane stability. There are almost 22 different sterols are found yet and the major phytosterols include ß-sitosterol, campesterol and stigmasterol. The objective of this study was to isolate and characterize the bioactive principles from the chloroform fraction of Argemone Mexicana L. Methods: The isolation was done using column chromatography using gradient elution with different mobile phases. The isolated compound was subjected to spectral analysis. Structure elucidation was carried out on basis of spectral analysis. Results: The chemical investigation of the chloroform fraction of aerial parts of belonging to the family Papaveraceae led to the isolation of stigmasterol. The isolated compounds were characterized using various spectroscopic data as well as chemical studies. Conclusion: From the spectral characteristics, the isolated compound from the chloroform fraction of aerial parts was confirmed to be stigmasterol. This is the first ever report of these stigmasterol compound from the chloroform fraction of aerial parts of Argemone mexicana.
Tropical Journal of Pharmaceutical Research, 2011
Achyranthes aspera is the important medicinal herb found as weed throughout tropical region of Nepal. It belongs to the family amaranthaceae. It is known as dattiwan in nepali, Apamarg in Sanskrit, prickly chaff flower in English and Naayuruvi in tamil. The medicinal plants are used for treatment of various diseases because of their safety and effectiveness. Though almost all of its parts are used in traditional systems of medicines, seeds, roots and shoots are the most important parts which are used medicinally. The major chemical constituents are carbohydrates, protein, glycosides, alkaloids, tannins, saponins, flavoides, lignin etc. The review reveals that wide numbers of phytochemical constituents have been isolated from the plant which possesses activities like antiperiodic, diuretic, purgative, laxative, antiasthmatic, hepatoprotective, anti-allergic and various other important medicinal properties. The plant is used in indigenous system of medicine as emenagogue, antiarthritic, antifertility, laxative, ecbolic, abentifacient, and antihelminthic, aphrodisiac, antiviral, anti-plasmodic, and antihypertensive, anticoagulant, diuretic and anti-tumor. It is also useful to treat cough, renal dropsy, fistula, scrofula, skin rash, nasal, infection, chronic malaria, impotence, fever, asthma, piles and snake bites. This plant is astringent, digestive, diuretic, laxative, purgative and stomachic. The juice of the plant is used in the treatment of boils, diarrhea, dysentery, hemorrhoids, rheumatic pains, itches and skin eruption. The methnolic extraction gives more yields then the alcohol and petroleum ether.
The Scientific World Journal, 2014
Adiantum capillus venerisis a medicinally essential plant used for the treatment of diverse infectious diseases. The study of phytochemical and antimicrobial activities of the plant extracts against multidrug-resistant (MDR) bacteria and medically important fungi is of immense significance. Extracts from the leaves, stems, and roots ofAdiantum capillus veneriswere extracted with water, methanol, ethanol, ethyl acetate, and hexane and screened for their antimicrobial activity against ten MDR bacterial strains and five fungal strains isolated from clinical and water samples. Ash, moisture, and extractive values were determined according to standard protocols. FTIR (Fourier transform infrared Spectroscopy) studies were performed on different phytochemicals isolated from the extracts ofAdiantum capillus Veneris. Phytochemical analysis showed the presence of flavonoids, alkaloids, tannins, saponins, cardiac glycosides, terpenoids, steroids, and reducing sugars. Water, methanol, and ethan...
Screening for Bioactive Compounds from Adiantum capillus-veneris L
2012
The aim of the present study was to assess the biological properties of A. capillus-veneris L. and identified the functional compounds in plant extracts. The antimicrobial activities were determined with broth dilution method using 4 Gram-positive bacteria, 2 Gram-negative bacteria and 1 fungus. The antioxidant activities were studied by employing various in vitro antioxidant assays such as DPPH and ABTS scavenging and Fe–Fe transformation method. MTT colori metric analysis was used to measure the cytocide rate on SGC-7901 cell line. The content of total phenolics, tannins and flavones were measured by Folin-Ciocalteu reagent and aluminum nitrate methods, respectively. Phytochemical compounds were analyzed by HPLC-DAD-MS/MS technique. EtOAc fraction exhibited broad spectrum antimicrobial activities against all tested microorganism especially against Candida albicans with the MIC of 0.95 μg/mL. Similarly, this fraction also exhibited the strongest antioxidant potential among tested s...
Chromatographic procedures for the isolation of plant steroids
Journal of Chromatography A, 2001
In this review, we consider the general principles and specific methods for the purification of different classes of phytosteroids which have been isolated from plant sources: brassinosteroids, bufadienolides, cardenolides, cucurbitacins, ecdysteroids, steroidal saponins, steroidal alkaloids, vertebrate-type steroids and withanolides. For each class we give a brief summary of the characteristic structural features, their distribution in the plant world and their biological effects and applications. Most classes are associated with one or a few plant families, e.g., the withanolides with the Solanaceae, but others, e.g., the saponins, are very widespread. Where a compound class has been extensively studied, a large number of analogues are present across a range of species. We discuss the general principles for the isolation of plant steroids. The predominant methods for isolation are solvent extraction/partition followed by column chromatography and thin-layer chromatography/HPLC.