Ultraviolet (UV) Sensitive Leukocyte Allostimulation Cross-Reacts with UV Resistant Sperm (original) (raw)

Journal of Immunology, 1979

Abstract

Our group has previously reported that human sperm cells are capable of stimulating allogeneic lymphocytes. We now report that the HLA-D on sperm cells is resistant to ultraviolet (UV) light in contrast to HLA-D on leukocytes, which is very sensitive to UV light in the dosage range employed in these experiments. Responder leukocytes were cultured with varying concentrations of stimulating blood leukocytes and varying concentrations of sperm cells washed free of the highly suppressive seminal plasma factor(s). Stimulator blood and sperm cells were exposed simultaneously to UV light delivered from a G.E. cold quartz No. G 15-T8 (2537 Å germicidal) lamp at doses varying from 800 to 25,000 ergs/mm2 as measured by an IL 770 photometer with a narrow band NB254 filter. Even the lowest dosage of UV completely or nearly totally abrogated the stimulating capability of leukocytes whereas sperm cells showed excellent stimulating ability with as much as 25,000 ergs/mm2. We have also been able to clonally prime lymphocytes with sperms. After 10 to 14 days in first or primary culture, sperm-stimulated blast-derived lymphocytes were recultured. The allogeneic responding lymphocytes showed accelerated 3HTdR incorporation to leukocytes from the original sperm donor. We conclude that the UV-resistant HLA-D on sperms share some determinants with the UV-sensitive HLA-D on leukocytes.

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