Methyl jasmonate vapor increases the developmentally controlled synthesis of alkaloids in Catharanthus and Cinchona seedlings (original) (raw)
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Phytochemistry, 1998
Methyl jasmonate\ a chemical inducer of secondary metabolism\ has been shown to promote vindoline biosynthesis in developing seedlings\ as a result of induction of tryptophan decarboxylase "TDC# and desacetylvindoline 3!hydroxylase "D3H#[ The present studies suggest that jasmonate!based induction of TDC and D3H activities involves modulation of transcriptional\ post!transcriptional and post!translational controls[ The e}ects of jasmonate on both enzymes were transient with maximum TDC activity appearing 01 h earlier than that of D3H[ Jasmonate treatment of etiolated seedlings neither enhanced TDC activity nor could it replace the light requirement for D3H induction[ Jasmonate\ therefore\ appears to modulate events which are already triggered by developmental and environmental speci_c controls[ Salicylic acid\ another chemical inducer of secondary metabolism\ was ine}ective in activating either TDC or D3H under the experimental conditions used[ Þ 0887 Elsevier Science Ltd[ All rights reserved
Precursor limitations in methyl jasmonate-induced Catharanthus roseus cell cultures
Plant Cell Reports, 2006
Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced with MJ (100 μM) on day 6 and fed loganin (0.30 mM), tryptamine (0.15 mM), loganin plus tryptamine, or geraniol (0.1–1.0 mM) on day 7. While MJ increased ajmalicine production by 3-fold, induced cultures were still limited by terpenoid precursors. However, both induced and non-induced cultures became tryptamine-limited with excess loganin. Geraniol feeding also increased ajmalicine production in non-induced cultures. But MJ appeared to increase geraniol availability in induced cultures, due presumably to the increased expression of Dxs with MJ addition.
Functional Plant Biology, 2020
To compare the effects of different carbon sources on physiological aspects, especially medicinal alkaloid biosynthesis and related gene expression in Catharantus roseus (L.) G.Don, we employed sucrose and sorbitol with two concentrations (87.64 mM, the equimolar concentration of sucrose in MS basal medium, and 150 mM) on the plant’s shoots in vitro in presence of 100 μM methyl jasmonate. The production of plant alkaloids including vincristine, vinblastine, ajmalicine, vindoline and catharantine and their biosynthetic and regulatory gene expression was measured. Both treatments had incremental effects on alkaloid production, upregulated the mitogen-activated protein kinase3 (MAPK3) and a downstream responsive transcription factor, ORCA3, which resulted in elevated transcript contents of the important genes in terpenoid indol alkaloids biosynthetic pathway including peroxidase1 (PRX1), geissoschizine synthase (GS), strictosidine synthase (STR) and deacetylvindoline acetyltransferase ...
Effect of culture process on alkaloid production by Catharanthus roseus cells
Journal of Biotechnology, 1991
The processes for production of indole alkaloids in shake flask suspension cultures of Catharanthus roseus cells using Zenk's alkaloid production medium (APM) were evaluated. The 1-stage process consisted of inoculating APM and incubating for 15 days. The 2-stage process involved 6 d of cultivation in growth medium followed by 15 d of incubation in APM. Growth, main nutrient consumption and alkaloid production were monitored. Both culture processes produced ~ 20 g dw per I of biomass. However, 2-stage cultures yielded an inorganic nutrient richer and more active plant cell biomass, richer in inorganic nutrients, as indicated by higher (> 70%) nutrient availability and consumption. Total and individual indole alkaloid production were 10 times higher (740 mg 1-~ and 25 to 4000 /xg per g dw, respectively) for 2-stage than for 1-stage cultures. For both processes, highest alkaloid productivity coincided with complete extracellular consumption of major inorganic nutrients, especially nitrate, by the cells. Complete carbohydrate consumption in 2-stage cultures resulted in a 40% decline in produc-Correspondence to: J. Archambault, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Ave., Montreal, Canada H4P 2R2. Abbreviations: 2,4 D: 2,4 dichlorophenoxyacetic acid; APM: alkaloid production medium; AB5:B5 growth medium with IAA; B5: plant cell basal growth medium; KLa: mass transfer coefficient; TIA: total indole alkaloid content; SM: secondary metabolites. 2 tion. Small but significant (~ 10%) product release was observed for both culture regimes, which seemed not to be related to cell lysis.
Indonesian Journal of Science and Technology, 2021
Cinchona alkaloids are known as antimalaria and anti-arrhythmic. Due to the long waiting time to harvest, cell culture technology is a challenge. This study aimed to determine the effects of elicitors, filtrate of two strains of endophytic fungi and methyl jasmonate (MeJA), in cell suspension culture of Cinchona ledgeriana on quinine and quinidine production. The cells were cultured for seven weeks in woody plant (WP) media treated with either of those elicitors in various concentrations. The cells growth was observed and the alkaloids were analyzed by HPLC. Cells treated with MeJA failed to grow that led to the cell biomass insufficiency for alkaloids determination. It indicates that the cells are quite sensitive to even low concentration of MeJA that hampered the growth. Cells treated with the filtrate of Diaporthe sp. M13-Millipore filtered (S2M) gave the least cell biomass but presented the highest content of both alkaloids. Diaporthe sp. strain M-13 is stronger as elicitor tha...
Journal of biotechnology, 1991
The processes for production of indole alkaloids in shake flask suspension cultures of Catharanthus roseus cells using Zenk's alkaloid production medium (APM) were evaluated. The 1-stage process consisted of inoculating APM and incubating for 15 days. The 2-stage process involved 6 d of cultivation in growth medium followed by 15 d of incubation in APM. Growth, main nutrient consumption and alkaloid production were monitored. Both culture processes produced ~ 20 g dw per I of biomass. However, 2-stage cultures yielded an inorganic nutrient richer and more active plant cell biomass, richer in inorganic nutrients, as indicated by higher (> 70%) nutrient availability and consumption. Total and individual indole alkaloid production were 10 times higher (740 mg 1-~ and 25 to 4000 /xg per g dw, respectively) for 2-stage than for 1-stage cultures. For both processes, highest alkaloid productivity coincided with complete extracellular consumption of major inorganic nutrients, especially nitrate, by the cells. Complete carbohydrate consumption in 2-stage cultures resulted in a 40% decline in produc
Biochemical Systematics and Ecology, 2019
The Annonaceae family presents alkaloids with ecological functions and pharmacological interest. This is the first study to evaluate if the application of plant growth regulators to seeds of this family alters the production of alkaloids over time from germination. This study was carried out in four sequential stages of Annona cacans Warm. development from the resumption of embryo during seed imbibition with the application of plant regulator GA 4+7 + 6-Benzyladenine. The concentration of total alkaloids was quantified using the oxoaporphine alkaloid liriodenine as reference standard. In addition, the liriodenine concentration was measured and the profile evaluated by ultra-high-performance liquid chromatography (UHPLC). Results have shown that alkaloids are present in all phases and in all tissues, at higher concentrations in roots (up to 100 times). The proportion of total alkaloids and liriodenine was modified in response to the application of plant regulators. Roots doubled the content of alkaloids and liriodenine. In cotyledonary leaves, the amount of total alkaloids decreased; however, liriodenine remained unchanged. Our results have shown that the use of plant growth regulators based on gibberellins and cytokinins modified the production of alkaloids in tissues in a specific way.
2005
Variations in alkaloid pattern during drying of leaves (leaf processing) showed that treatment with methyljasmonate can induce formation of bisindole alkaloids as a result of catabolism of the monomeric alkaloids catharanthine and vindoline. A twofold increase in 3V,4V-anhydrovinblastine was shown in treated leaves especially from day 8 until day 21. Serpentine also increased in the same period under the treatment as a catabolic product of ajmalicine. Basic peroxidases that are responsible for the formation of anhydrovinblastine and serpentine showed high activity at days 8 and 21 in treated leaves, causing the increase in anhydrovinblastine and serpentine. D