Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells to Osteoblast Cell in Early, Middle and Late Passages (original) (raw)

2018, Journal of Stem Cell Research & Therapy

Human adipose tissue is a great source of mesenchymal stem cells. Adipose-derived mesenchymal stem cells (AD-MSCs) are easily isolated, able to differentiate into multi-lineage cells and have various clinical application. This promising potential will be more valuable for the application of tissue engineering if they can be sequentially subcultured without loss of phenotype and multilineage differentiation ability. Thus, in this study we investigated the ability of human AD-MSCs to differentiate into osteogenic lineage by performing histological staining and studied the osteogenic potential of human AD-MSCs on early (P2, P5), middle (P10) and late passage (P15). Cells were cultured in osteogenic medium for 2 weeks and characterized at 1, 4, 7, 10 and 14 days. During sequential subcultivation, AD-MSCs were able to maintain their osteogenic potential through late passage. AD-MSCs in middle passage exhibited a better osteogenic potential than the early and late passages, while the AD-MSCs from P2 are less differentiated than middle and late passages. The result that was found in this research should be accounted for when developing stem cell-based therapies for clinical application