Assessment of dental plaque coverage by Quantitative Light-induced Fluorescence (QLF) in domestic short-haired cats (original) (raw)
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Quantification of Canine Dental Plaque Using Quantitative Light-Induced Fluorescence
Journal of veterinary dentistry, 2016
The aim of this work was to evaluate Quantitative Light-induced Fluorescence (QLF) as an alternative to the established Logan and Boyce method for determining plaque coverage of dogs' teeth. In a series of studies in conscious and anesthetized dogs, QLF showed good intra-photographer repeatability (coefficient of variation [CV] of 7.5% for undisclosed teeth) and inter-photographer reproducibility (CV of 3.2% for undisclosed teeth and 8.5% for disclosed teeth). The QLF software accurately identifies areas of plaque as demonstrated by comparison to the variability of 5 human scorers, manually marking plaque on QLF-acquired images (P = 0.1). There was good agreement with the modified Logan and Boyce method in the percentage reduction in plaque accumulation measured when dogs were fed an oral care chew versus no chew. To see a 15% difference in plaque accumulation, which is considered sufficient by the Veterinary Oral Health Council to differentiate between 2 treatments, a retrospec...
Validation of Quantitative Light-Induced Fluorescence for Quantifying Calculus on Dogs’ Teeth
Journal of Veterinary Dentistry, 2018
Periodontal disease is a common disease of dogs and is initiated by the buildup of plaque on the tooth surface. As plaque matures, it becomes mineralized to form calculus, which although not directly involved in the etiology of periodontal disease, provides an irregular surface to which plaque can adhere. Evaluation of the quantity of plaque and calculus on dogs' teeth is therefore essential to enable the efficacy of products, designed to prevent or retard plaque and calculus accumulation, to be evaluated. The objective of this study was to determine whether quantitative light-induced fluorescence (QLF TM) is a suitable tool to quantify the amount of calculus on the buccal surface of dogs' teeth following the removal of disclosed plaque by tooth brushing. The amount of calculus on the teeth of 26 miniature schnauzers was measured, using QLF and a calculus index method (Warrick-Gorrel), during a 28-day phase crossover study comparing feeding a daily dental chew versus providing no daily chew. Quantification of calculus using the Warrick-Gorrel method showed a 43.8% reduction in calculus buildup, with 95% confidence interval of 27.3 to 60.3 (P < .001). With QLF, the percentage reduction in calculus accumulation was 65.8% (58.1-73.4, P < .001). A retrospective sample size analysis showed that fewer dogs were required for QLF analysis compared to the Warrick-Gorrel method. This study demonstrated that QLF is a sensitive and precise method for quantification of calculus on dogs' teeth. It removes the subjective element of human examiners and has greater accuracy and reduced variability through the continuous nature of the data.
Evaluation of the Logan & Boyce plaque index for the study of dental plaque accumulation in dogs
Research in Veterinary Science, 2006
The objectives of this study were to assess intra-examiner (experienced examiner) and inter-examiner agreements (experienced versus non-experienced examiners) of scores assessed with the Logan & Boyce plaque index and to evaluate whether a modification of this index, where anatomical landmarks are used for horizontal division [mod L&B-AL] and dye references are used for assessing intensity of dye (plaque thickness) [mod L&B-DR], would improve repeatability. The Logan & Boyce index was found to be inaccurate when scoring plaque coverage as it underestimated the total crown surface. The contribution of the gingival part to the total tooth score was minimized by the Logan & Boyce index compared to the mod L&B-AL/DR. Precision of global plaque scorings was significantly improved by the mod L&B-AL/DR. Intra-examiner agreement of plaque thickness and plaque coverage scorings on the gingival part of the tooth was significantly improved by the mod L&B-AL/DR. Studies evaluating plaque accumulation in dogs should therefore use the mod L&B-AL/DR rather than the Logan & Boyce index.
Quantification of dental plaque in the research environment
Journal of Dentistry, 2005
Objectives. To review the established and novel methods of plaque quantification employed in dental research, including a discussion of their merits and to present a new method of planimetrically measuring plaque using light induced fluorescence.
Reliability and discriminatory power of methods for dental plaque quantification
Journal of Applied Oral Science, 2010
OBJECTIVE: This in situ study evaluated the discriminatory power and reliability of methods of dental plaque quantification and the relationship between visual indices (VI) and fluorescence camera (FC) to detect plaque. MATERIAL AND METHODS: Six volunteers used palatal appliances with six bovine enamel blocks presenting different stages of plaque accumulation. The presence of plaque with and without disclosing was assessed using VI. Images were obtained with FC and digital camera in both conditions. The area covered by plaque was assessed. Examinations were done by two independent examiners. Data were analyzed by Kruskal-Wallis and Kappa tests to compare different conditions of samples and to assess the inter-examiner reproducibility. RESULTS: Some methods presented adequate reproducibility. The Turesky index and the assessment of area covered by disclosed plaque in the FC images presented the highest discriminatory powers. CONCLUSION: The Turesky index and images with FC with disclosing present good reliability and discriminatory power in quantifying dental plaque.
The Angle Orthodontist, 2016
Objective: To assess the use of Quantitative Light-induced Fluorescence-Digital as an oral hygiene evaluation tool during orthodontic treatment. Materials and Methods: In this prospective, randomized clinical trial, 33 patients undergoing fixed orthodontic appliance treatment were randomly allocated to receive oral hygiene reinforcement at four consecutive appointments using either white light (WL) or Quantitative Light-induced Fluorescence-Digital (QLF) images, taken with a device, as visual aids. Oral hygiene was recorded assessing the QLF images for demineralization, by fluorescence loss (ΔF), and plaque coverage (ΔR30). A debriefing questionnaire ascertained patient perspectives. Results: There were no significant differences in demineralization (P = .56) or plaque accumulation (P = .82) between the WL and QLF groups from T0 to T4. There was no significant reduction in demineralization, ΔF, in the WL, or the QLF group from T0–T4 (P > .05); however, there was a signific...
Journal of the Irish Dental Association, 2017
Statement of the problem: Fluorescein is a plaque detection agent, which fluoresces yellow-green when excited with blue light (dental light curing lamp). Little is known about the reproducibility of scoring with the Silness-Löe plaque index (1964) when using this agent. Purpose of the study: To evaluate the level of agreement of the plaque index measurements using a fluorescent colouring agent among eight dentists. Materials and methods: Eight dentists in Cork were recruited as examiners for a randomised clinical study investigating the impact of a personalised caries prevention approach. They were trained and calibrated in the use of the plaque index using Plaque Test (Ivoclar Vivadent, Liechtenstein) in the Oral Health Services Research Centre and School of Dental Hygiene, University College Cork. For inter-examiner and intra-examiner reproducibility, a previously calibrated 'gold standard' examiner and seven dentists examined 10 to 12 subjects each, while one dentist examined four subjects only for inter-examiner reproducibility. The adult subjects were recruited at the Cork University Dental School and Hospital. To evaluate inter-examiner and intra-examiner reproducibility at site level, squared weighted kappa statistics were calculated. Results: The weighted kappa statistics varied from 0.31 to 0.54 for inter-examiner reproducibility under the acceptable level (kappa statistics = 0.60) for research purposes and from 0.43 to 0.65 for intra-examiner reproducibility. Conclusions: The levels of agreement were fair to good. Further studies are needed, preferably including a qualitative study to analyse feedback from dentists to determine the cause of such variation. This study re-emphasises the importance of clinician calibration ahead of clinical studies.
BMC Oral Health
Background The effects of smoking on the accumulation of dental plaque have not been studied in depth. We compared dental plaque quantitation obtained with a novel light induced fluorescence technology among current, former, and never smokers and verified measurements’ repeatability. Methods Dental plaque quantitation was objectively assessed by quantitative light induced fluorescence (QLF) technology on three separate study visits in current, former, and never smokers: baseline (day 0), day 7, day 30. Increase in the fluorescence intensity of at least 30% (ΔR30) and 120% (ΔR120) together with the simple oral hygiene (SOH) scoring were considered for analysis. Results The QLF parameters were highly repeatable in each study group (p < 0.0001, by regression analyses). All QLF parameters showed a significant difference between never smokers and current smokers (p = 0.041 for ΔR30; p = 0.027 for ΔR120; p = 0.04 for SOH). No significant differences were observed between never and form...
Quantified light-induced fluorescence, review of a diagnostic tool in prevention of oral disease
Journal of Applied …, 2009
Diagnostic methods for the use in preventive dentistry are being developed continuously. Few of these find their way into general practice. Although the general trend in medicine is to focus on disease prevention and early diagnostics, in dentistry this is still not the case. Nevertheless, in dental research some of these methods seem to be promising for near future use by the general dental professional. In this paper an overview is given of a method called quantitative light-induced fluorescence or ͑QLF͒ in which visible and harmless light excites the teeth in the patient's mouth to produce fluorescent images, which can be stored on disk and computer analyzed. White spots ͑early dental caries͒ are detected and quantified as well as bacterial metabolites on and in the teeth. An overview of research to validate the technique and modeling to further the understanding of the technique by Monte Carlo simulation is given and it is shown that the fluorescence phenomena can be described by the simulation model in a qualitative way. A model describing the visibility of red fluorescence from within the dental tissue is added, as this was still lacking in current literature. An overview is given of the clinical images made with the system and of the extensive research which has been done. The QLF™ technology has been shown to be of importance when used in clinical trials with respect to the testing of toothpastes and preventive treatments. It is expected that the QLF™ technology will soon find its way into the general dental practice.
BMC oral health, 2017
Significant numbers of adults and children have untreated plaque due to poor oral hygiene and consequently suffer from associate dental and systemic diseases. A handheld device equipped with 405 nm light-emitting diodes was constructed to examine the prevalence of red fluorescence signatures associated with dental plaque. This device was used for in vivo imaging of all four incisors and all four canines of twenty-eight consenting human subjects. The same areas were further imaged under white light illumination with a commercial image-processing based plaque-imaging device, and evaluated by a hygienist and dentist. A custom computer vision algorithm using pixel information was developed to calculate plaque coverage ratios ranging from 0 (no plaque) to 1 (complete plaque coverage) for images captured by both devices. The algorithm calculated red fluorescence-based plaque coverage ratios ranging from 0.011 to 0.211 for the subjects imaged. Clinical assessment and statistical analyses o...