Comparative Evaluation of Polyphenol Contents and Biological Activities of Five Cistus L. Species Native to Turkey (original) (raw)
Related papers
Plants (Basel, Switzerland), 2017
The in vitro antioxidant potential assay between ethanolic extracts of two species from the genus Vitex (Vitex negundo L. and Vitex trifolia L.) belonging to the Lamiaceae family were evaluated. The antioxidant properties of different extracts prepared from both plant species were evaluated by different methods. DPPH scavenging, nitric oxide scavenging, and β-carotene-linoleic acid and ferrous ion chelation methods were applied. The antioxidant activities of these two species were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and Ethylene diamine tetra acetic acid (EDTA). Both species of Vitex showed significant antioxidant activity in all of the tested methods. As compared to V. trifolia L. (60.87-89.99%; 40.0-226.7 μg/mL), V. negundo has been found to hold higher antioxidant activity (62.6-94.22%; IC50 = 23.5-208.3 μg/mL) in all assays. In accordance with antioxidant activity, total polyphenol contents in V. negundo possessed greater phen...
2011
he methanolic extract of Vitex negundo L. was dissolved in distilled water and partitioned with nexane, chloroform, ethyl acetate and n-butanol successively. The antioxidant potential of all ese fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2icrylhydrazyl radical (DPPH) scavenging activity, total antioxidant activity, ferric reducing ntioxidant power (FRAP) assay and ferric thiocyanate assay along with the determination of total henolics. The results revealed that ethyl acetate soluble fraction showed highest percent cavenging of DPPH (86.0 ± 0.33%) at the concentration of 30 μg/ml. The IC50 value of this action was (15.54 ± 0.10 μg/ml) relative to butylated hydroxytoluene (BHT), having IC50 of 12.1 ± .92 μg/mL. Chloroform soluble fraction was found to have the highest total antioxidant activity 2.071 ± 0.13). Ethyl acetate soluble fraction also exhibited highest FRAP value (3367.8 ± 5.89 μg f trolox equivalents), highest concentration of total...
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
The study was aimed to comparatively evaluate Total Phenolic Content, Total Flavonoid content and DPPH free radical scavenging activity of the methanolic extracts of different plant parts (leaves, stems, flowers, fruits and roots) of Vitex negundo. Methods: In this study, quantitative estimation of total phenolic and flavonoid contents were performed by using Folin-Ciocalteu method and aluminum chloride colorimetric method respectively. The free radical scavenging activities of the methanolic extracts of different plant parts were examined using established DPPH (2,2-Diphenyl-1-Picrylhydrazyl) assay. Results: The highest Total Phenolic Content was found in flowers (71.21 ± 0.855 mg Gallic Acid Equivalent/ g dry weight of extract) while Total Flavonoid content was observed in leaves (27.32 ± 0.205 mg Quercetin Equivalent/g dry weight of extract). The highest free radical scavenging activity was recorded in flowers with IC50 Conclusion: These findings suggest that methanolic extracts of different plant parts of Vitex negundo contain significant amount of phenols and flavonoids and also have significant antioxidant activity therefore it can be used in formulation of many antioxidant products and can be used as an important source of natural antioxidants. value (82.25 ± 0.174 µg/ml) thus the most potent antioxidant part of the plant. Free radicall scavenging activity of the extracts was also found to increase in a dose dependent manner.
Journal of Chemical Health Risks, 2017
In this study, the essential oil obtained by hydrodistillation of the seeds of Vitex pseudo-negundo (Verbenaceae), growing wild in Sabzevar, Khorasan Razavi Province, Iran, were analyzed by GC and GC/MS. The yield of total volatiles was 0.8% (w/w). Thirty-seven compounds representing 92.4% of the seed oil were identified. The main components of the oil were hexadecanoic acid (8.0%), elemol (7.0%) and α-bisabolol (6.1%). The oil was rich in sesquiterpene hydrocarbons (51.2%). The total flavonoids of different extracts of the plant were found the range 56-195 mg/g, with the maximum amount being in the methanol extract. In addition, the antioxidant activities of the extracts were measured by radical scavenging activity of antioxidants against the free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH). The antioxidant activities of the extracts of V. pseudo-negundo seed were higher than those of the standard synthetic antioxidants, BHT, ascorbic acid and gallic acid.
Studies on Analysis of Antioxidant and Enzyme Inhibitory Activity of Vitex negundo Linn
International Journal of Pharmacognosy and Phytochemical Research, 2017
The current study was designed to investigate the leaf extracts of Vitex negundo Linn. for their antioxidant and enzyme inhibitory (α-amylae and urease) activity. The antioxidant capacity of the different extracts (methanol, acetone and aqueous) of this plant was evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl) and reducing power tests. The plant exhibited good DPPH radical scavenging activity and moderate reducing power potential Further, all the extracts of V. negundo were reported to possess good anti-alpha amylase and anti-urease activity of greater than 50% in all the solvents used at a concentration of 1 mg/mL. Thus the study provided scientific evidence to the traditional uses of this plant in the treatment of obesity, diabetes, ulcers, kidney stones etc. Therefore, the leaf extracts of this plant can be selected for further investigation to determine their therapeutic potential.