Various Concentration Distribution of Benzil Amino Purine Concentration on Callus Growth in Apple Cucumber Originated from Karawang (original) (raw)

2022, Jurnal Mangifera Edu

Apple cucumber have relatives with melon as seen from squencing using the Internal Transribed Spacer (ITS) method. The lack of data regarding the morphology of the apple cucumber causes the absence of information on the morpho-agronomy of the apple cucumber. The purpose of the study is to find the best Benzil Amino Purine (BAP) concentrations and proper explants for the growth of the apple cucumber callus (Cucumis sp.) on Murashige and Skoog (MS) media. Research on direct of part apple cucumber was conducted from March until May 2019. The methods used was an experimental method with non parametric statistic with 8 treatments: A0B1 (BAP 0 + Leaves), A0B2 (BAP 0 + Stem), A1B1 (BAP 0.5 μM + Leaf), A1B2 (BAP 0.5 μM + Stem), A2B1 (BAP 1.5 μM + Leaf), A2B2 (BAP 1.5 μM + Stem), A3B1 (BAP 3.0 μM+ Leaves) and A3B2 (BAP 3.0 μM+ Stems) that were repeated 5 times and analyzed descriptively using the Kruskal Wallis Test. The results showed that callus growth of apple cucumber at the best concentartion BAP and exsplant that is at concentration 0.5 μM BAP + stem which exerted a faster effect on callus formation time (27 hsi), 1.5 μM BAP + stem giving the largest diameter with 1.2 cm with crumb and yellowish-green morphology. The stem explant gives the best growth rate (0.887 cm/3 day) on 0.5 μM and 1.5 μM concentration.

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