The Study on Effect of Temperature Stress on Occurrence of Clinical Signs Caused by Aeromonas hydrophila in Capoeta damascina in In Vitro Condition (original) (raw)
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E3S Web of Conferences, 2022
Aeromonas hydrophila is a bacterial pathogen in freshwater aquaculture causing the motile aeromonad septicemia (MAS) disease and inflict significant economic losses. This study aimed to determine the lethal concentration (LC50-48h) of A. hydrophila on Tor soro's fingerlings and investigated the clinical signs that appeared following the challenge test. Briefly, 150 fingerlings (3,4 ± 0,18 cm) were challenged by immersion means with 0 (control), 104, 105, 106 and 107 CFU/mL of live cells of A. hydrophila. Reed and Muench’s method’s determination of LC50-48h was calculated and all clinical signs that appeared during the test were recorded. The result showed that the LC50-48h by immersion was 1.0 x 106,66 CFU/mL with clinical signs observed as hemorrhages, depigmentation, lesions, anorexia, loss of balance, and enlargement of the abdominal cavity. Furthermore, based on the LC50-48h and the clinical symptoms, this study reveals that even though all symptoms are consistent among the fish, the severity of the disease tends to be prevalent and intense as the concentration of pathogen is higher.
2018
A study was initiated to evaluate the performance and hematological responses of Nile Tilapia FaST Strain Oreochromis niloticus (L) to an experimental infection with Aeromonas hydrophila. The experiment consisted of two treatments with three replicates: Treatment 1 (Control)/non-injected fish (NI); Treatment 2 (Infected)/fish injected with Aeromonas hydrophila. Twenty-four hours after injection, the fish were anesthetized and the blood was collected. The hematological test included white blood cell (WBC) counts in treatments. Fish injected with Aeromonas hydrophila showed significant difference in white blood cell (WBC) counts than the non-injected treatment. Final weight and weight gain increased significantly in fish injected when compared to non-injected control. Statistical analysis of survival rate showed significant difference between treatments (P<0.05). Treatment 1 (Control) has significantly higher survival rate which was 75% compared to Treatment 2 (Infected) which was ...
A study was initiated to evaluate the performance and hematological responses of Nile Tilapia FaST Strain Oreochromis niloticus (L) to an experimental infection with Aeromonas hydrophila. The experiment consisted of two treatments with three replicates: Treatment 1 (Control)/non-injected fish (NI); Treatment 2 (Infected)/fish injected with Aeromonas hydrophila. Twenty-four hours after injection, the fish were anesthetized and the blood was collected. The hematological test included white blood cell (WBC) counts in treatments. Fish injected with Aeromonas hydrophila showed significant difference in white blood cell (WBC) counts than the non-injected treatment. Final weight and weight gain increased significantly in fish injected when compared to non-injected control. Statistical analysis of survival rate showed significant difference between treatments (P<0.05). Treatment 1 (Control) has significantly higher survival rate which was 75% compared to Treatment 2 (Infected) which was 25%.
Journal of Bio-Science, 2009
Among the fishery resources, swamps are very important for various fish species such as Oreochromis nilotica, Oreochromis mossambica, Anabas testudineus, Channa punctatus and Heteropneustes fossilis. Swamps require shorter time and small investment for fish culture (Rahman et al. 1998). However, diseases are major problems in fish production both in culture system and wild condition in Bangladesh (Rahman and Chowdhury 1996). Bacteria, especially Aeromonas sp. is one of the causative agents of fish diseases. Bangladesh is situated in the tropical zone. Temperature is one of the important factors which affect the growth of pathogenic bacteria (Ljungh and Wadstrom 1982). Aeromonas sobria increases and grows at 17- 25°C (Rahman and Chowdhury 1996). Aeromonas sp. is the causative agent of various kinds of ulcerative disease of fishes (Karunasagar and Sugumar 1995). Yesmin et al. (2004) and Chowdhury and Baqui (1997) reported that Aeromonas is a very common pathogen in carps and live fis...
Iranian Journal of Fisheries Sciences, 2012
In this research the affect of A. hydrophila on pathogenesis of Crayfish A. leptodactylus was carried out. This study was designed in three groups as treatments and one group as control in triplicate with 20 Crayfish in each glass aquarium. The treatments have exposed to A. hydrophila. The concentrations of the bacteria in treatments were , and CFUmL-1 respectively. The untreated control group was disinfected by oxytetracyclin at concentration of 100 ppm for 24 hours. The haemolymph samples were withdrawn for measuring of THC and TPC within interval hours (2, 6, 12, 24, 48, 96, 144, 240 and 336). The Crayfish samples were then fixed in Davidson fixative. The results showed that the differences of THC value between treatment 4 (95.33±23.16) and control (1.13±.45) were significant (P < 0.05) as well as control and treatments 3 (35.33±16.16) and 4 (95.33±23.16) during 2 initial hours. In case of THC the significant difference (P < 0.05) was observed between control group (72.3...
2020
Fish, the fastest food producing sector in the world is one of the richest sources of animal protein. However, diseases are the major bottleneck which causes severe economic loss. Therefore routine surveillance of diseases is extremely important to predict and minimize the adverse effect caused by outbreak, epidemic, and pandemic situations, by adopting suitable and accurate preventive measures. In recent times, serosurveillance the gold standard programme is routinely used to measure the population immunity which in turn complements the traditional disease surveillance methods. Serological studies used to investigate the acquisition of various infections in a population, measure the induction of an immune response in the host, evaluate the persistence of antibody etc. Herein, for the first time possibly an attempt has been made to study the seroprevalence of Aeromonas hydrophila which is widely associated with a wide range of diseases like epizootic ulcerative syndrome, dropsy, fin...
Water temperature was set up at 2 levels; high (Ht, 29.5±0.5 o C) and low temperature (Lt, 19.5±0.5 o C). Experimental catfish body weight and total length were 12.07±1.41 g and 11.57±0.62 cm respectively. Catfish were divided into 4 groups: high temperature with 0.1% sodium chloride (NaCl) (HtWs), high temperature without 0.1% NaCl (HtW/s), low temperature with 0.1% NaCl (LtWs), and low temperature without 0.1% NaCl (LtW/s). Catfish hematocrit (Hct) and clinical chemistry including serum glutamic oxaloacetic transminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), and creatinine (Cr) were measured. The results showed that water temperature, Ht (HtWs and HtW/s) and Lt (LtWs and LtW/s), disturbed homeostasis. The Hct values showed that fish from LtW/s and LtWs group potentially were anemic fish much more than fish from HtWs and HtW/s group. The BUN to Cr ratio reveals that all fish in HtWs and LtWs group were dehydrated when compared to fish in HtW/s and LtW/s group. Ten isolates Aeromonas hydrophila (A. hydrophila) were intra-peritoneal injection to catfish which exposed to HtW/s and LtW/s. All A. hydrophila were isolated from kidney and liver of sick fresh-water fish in Thailand from 2005 to 2008. The virulence of A. hydrophila to catfish exposed to LtW/s and HtW/s were 100% and 40% respectively. The results demonstrated water temperatures play a major role to catfish hematology and A. hydrophila virulent levels. The evaluation of blood chemistry parameters provide a baseline and as a tool in fish health management.
Archives of Razi Institute, 2019
Bacterial diseases in cultured fish are considered the main problem with aquaculture system in Iran. The gills are multifunctional organs responsible for respiration, osmoregulation, nitrogenous waste excretion, and acid-base balance. Moreover, they are very sensitive to water contamination. Aeromonas hydrophila (A. hydrophila) is an opportunist pathogen responsible for a wide range of diseases in different species of fish. The gill histological alterations were used to assess the effects of A. hydrophila exposure on yellowfin sea bream, Acanthopagrus latus (A. latus). In this regard, 90 A. latus were exposed to the concentrations of A. hydrophila (103 and 106 CFU/ml) for three weeks. The most histopathological alterations in the gill of the exposed fish included hypertrophy and hyperplasia of the epithelial cells, lamellar fusion, club shaping of gill lamellae, lifting of the epithelium and edema of lamellae with large sub-epithelial space, blood congestion, and hypertrophy and hyp...
Agricultural Science Digest, Volume 43 Issue 2: 255-259 (April 2023)
Background: The floating cage cultured seabass (Lates calcarifer) at the site of Valaichennai lagoon in Batticaloa District, East coast of Sri Lanka was reported acute mortality within short period of stocking the fish in the cage. Methods: Present study was focused to study the aetiology that caused by infection on culturing sea bass at the site. Gross pathological and histopathological examinations were carried in moribund and naïve fishes. Bacteriological, mycological screening and antibiotic sensitive test for isolation of pathogen were carried out to find out the causes. Water quality, feed quality and their management were assessed at the time of disease outbreak. Result: Investigations primarily revealed that infection was initiated with the flooding due to heavy monsoonal season and sudden fluctuation in salinity. The gross pathological signs were change of skin colour, large irregular haemorrhages on the body surface, cloudy eye, ulcers on skin, lamellar fusion, fin rot and tail rot as well as histopathological findings such as necrosis in muscles, liver and kidney tissues, cyst formation by secondary infected pathogens proved that pathogen was bacteria. Gram staining also offered clear indication of gram-negative rod-shaped bacteria and, the particular pathogenic bacteria was confirmed as Aeromonas hydrophila through further analyses such as oxidative test, indole test and antibiotic sensitivity test. Antibiotic sensitivity test also point out that the Tetracycline as one of the effective antibiotics for the Aeromonas hydrophila infection. The improper feeding management and sudden salinity fluctuation pave the path for acute infection of A. hydrophila and ultimately lead to the high mortality in cage culture.