Antihyperglycaemic and antioxidant activities of Crataeva adansonii DC. ssp. adansonii leaves extract on ICR mice (original) (raw)
Related papers
Antioxidant and antidiabetic activities of medicinal plants: A short review
Diabetes mellitus being a chronic endocrine disorder has become a major health care problem since the last couple of decades because of the intensive lifestyle intervention. Antioxidants have become a crucial part of our lives for maintaining optimal cellular and systemic health and wellbeing. There is an increased interest in the food industry and preventive medicine in the development of natural antioxidants from plant material. This review deals with medicinal plants with antioxidant and antidiabetic properties used in the traditional Indian system of medicine; also a briefing of their in vitro models for evaluating antioxidant and antidiabetic activity has been con-ducted. At the same time, recent work done on the pharmacological aspects of these medicinally important plants has been iterated in this review.
Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property
Journal of ethnopharmacology, 2002
Methanolic extract (75%) of Terminalia chebula , Terminalia belerica , Emblica officinalis and their combination named 'Triphala' (equal proportion of above three plant extracts) are being used extensively in Indian system of medicine. They were found to inhibit lipid peroxide formation and to scavenge hydroxyl and superoxide radicals in vitro. The concentration of plant extracts that inhibited 50% of lipid peroxidation induced with Fe 2' /ascorbate were food to be 85.5, 27, 74 and 69 mg/ml, respectively. The concentration needed for the inhibition of hydoxyl radical scavenging were 165, 71, 155.5 and 151 mg/ml, and that for superoxide scavenging activity were found to be 20.5, 40.5, 6.5 and 12.5 mg/ml, respectively. Oral administration of the extracts (100 mg/kg body weight) reduced the blood sugar level in normal and in alloxan (120 mg/kg) diabetic rats significantly within 4 h. Continued, daily administration of the drug produced a sustained effect. #
The antioxidant activity of several antidiabetic herbal products
Pharmaciana
The oxidative stress mechanism in the body involves the balance between increased ROS and decreased antioxidant agents. ROS can damage DNA, proteins, and lipids, leading to pathology and impaired insulin secretion by dysregulation of several genes expression. High concentrations of ROS are often followed by a low activity of antioxidants from endogenous sources. As a result, exogenous antioxidants are required to neutralize ROS. As reported, medicinal plants, which have had various traditional applications, contain large amounts of secondary metabolites, proven to be exogenous antioxidant agents. Jamu, a traditional medicine, also known as an alternative medication, can be widely and easily found in traditional markets. Antidiabetic herbal products are among the most popular of jamu. Antidiabetic mechanism has a strong relationship with antioxidant roles in many biological systems. The aim of this research was to figure out the potential antioxidant activity of antidiabetic herbal products. Eight antidiabetic jamu samples were analyzed for their antioxidant activity by measuring the DPPH radical scavenging activity, phosphomolybdenum antioxidant activity, and FRAP, as well as for their total phenolic and flavonoid contents. Good linearity was a general finding for the quantitative analysis, with an average correlation coefficient of 0.999 for the standards. The highest total flavonoid content found was 8.66 ± 0.11 mgQE/g, and the highest total phenolic content was 22.66 ± 0.15 mgGAE/g. With regard to antioxidant activity, all of the samples demonstrated weak DPPH radical scavenging activity, while phosphomolybdenum antioxidant activity and FRAP were the highest at 48.58 ± 0.45 mgQE/g extract and 226.98 ± 0.19 mgQE/g extract, respectively. These results indicated that the claimed antidiabetic herbal products could be prepared as exogenous antioxidant sources, irrespective of being with or without antioxidant activity, for diabetes treatment.
BioMed Research International, 2019
Traditionally, in many countries, various parts of the Adansonia digitata (A. digitata) tree have been used in the treatment of many clinical ailments including diarrhea and dysentery. The phytochemical screening has indicated that the leaf extract of A. digitata contains flavonoids, saponins, mucilage, steroids, and alkaloids. Thus, this paper aims to evaluate the hyperglycaemic and hypolipidaemic effects of methanolic extract of A. digitata leaves (200 mg/kg and 400 mg/kg) in diabetic rats. The extract was administered orally for six weeks in the streptozotocin (STZ)-induced diabetic rats. The treatment with the extract caused a significant reduction in the blood glucose, glycosylated hemoglobin, cholesterol, triglycerides, low-density lipoprotein (LDL), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-), and malondialdehyde (MDA) levels by 46.7%, 46.15%, 48.91%, 43%, 60%, 66%, 45.45%, and 30.4%, respectively, as compared to the diabetic group after the sixth week of treatment. The leaf extract also mitigated the decline of high-density lipoprotein (HDL) level, RBCs count, hemoglobin level, packed cell volume (PCV %), and erythropoietin concentration in diabetic rats by 31%, 33.25%, 24.72%, 51.42%, and 220.68% with respect to the diabetic group. Also, the extract maintained the level of antioxidant enzymes, catalase (CAT) and superoxide dismutase (SOD), and reduced glutathione (GSH) in the diabetic rats. It also reduced the elevation in the white blood corpuscles (WBC) count in the STZ-induced diabetic rats. Our study, therefore, indicates that methanolic extract of A. digitata leaf exerts strong antidiabetic and hypolipidaemic properties in a dose-dependent manner by improving the hematological properties and redox parameters in the experimental diabetic rats.
2024
The antidiabetic and antioxidant efficacy of traditional medicinal plants from NorthEastern India remains largely unexplored. This study seeks to address this gap by examining the properties of 11 such plants. The plant samples were subjected to extraction using four different solvents. Their antidiabetic potential was evaluated through in-vitro α-glucosidase and α-amylase inhibition bioassays, while the antioxidant activity was assessed using the DPPH Scavenging assay. The results were compared to the standard antidiabetic drug, acarbose. Out of the 11 plants, five species demonstrated significant antidiabetic properties, inhibiting more than 50% of α-glucosidase and α-amylase enzyme activities. The petroleum ether tuber extract of Stephania glandulifera exhibited the highest α-glucosidase inhibition (99%), followed by the methanol leaf extract of Begonia roxburghii (94%) and the water leaf extract of Antidesma acidum (85%). In the α-amylase inhibition assay, Equisetum debile methanol extracts showed a 64% inhibition, followed by the leaf extract of A. acidum with a 62% inhibition in the methanol leaf extracts. Comparatively, methanol and water leaf extracts of Debregeasia longifolia displayed 64% and 67% inhibitions, respectively. The water extract of B. roxburghii demonstrated significant antioxidant activity (57%), while the other samples showed less than 50% effectiveness. Notably, the α-glucosidase inhibition consistently exceeded the α-amylase inhibition across the samples. This study confirms the antidiabetic properties of five out of the 11 ethnomedicinal plants, as evidenced by the in-vitro α-glucosidase and α-amylase inhibitory assays. These findings support the traditional use of these plants in the management of diabetes and emphasize the need for further in-vivo studies, which could contribute to the development of novel herbal formulations or therapeutic drugs for the treatment of diabetes.
African Journal of Traditional, Complementary and Alternative Medicines, 2015
Background: Over the last decade, extensive research work has focused on the potential health benefits of antioxidants while many medicinal plant extracts have been evaluated for their antioxidant profile. Medicinal plants selected for this study are widely used in traditional medicine for the treatment of diabetes mellitus in Sri Lanka some of which are recommended as dietary supplements to the existing therapies. The present study aimed at determining the total polyphenol contents and total antioxidant activities of aqueous extracts of 10 selected Sri Lankan medicinal plants by three in vitro methods; Materials and Methods: DPPH(2, 2-diphenyl-2-picrylhydrazyl), FRAP (ferric reducing power) and NO (nitric oxide) assays. The aqueous plant extracts were tested at the concentration of 0.05 g/mL. The total polyphenol content was determined according to the Folin-Ciocalteu method while the total antioxidant activity was evaluated by DPPH, FRAP and NO assays with L-ascorbic acid as refer...
Pharmacia
The focus of the presented study is the in vitro anti-oxidant activity and anti-diabetic potential of water extracts from the following four herbal substances, not traditionally used for treatment of diabetes mellitus – leaves of Sambucus ebulus L. and Prunus mahaleb L., and flowering stems of Cichorium intybus L. and Satureja kitaibelii Wierzb. ex Heuff. The water extracts are obtained through ultrasonication. The extract of S. kitaibelii stands out due to its highest values in all studied indicators – total phenolic content, scavenging potential (DPPH, ABTS) and α-glucosidase inhibitory activity which was six times higher than acarbose. The extract of C. intybus also showed significant α-glucosidase inhibitory activity compared to acarbose. The flowering stems of both species are promising sources of biologically active substances for blood sugar control in diabetes mellitus.
Asian journal of Chemistry, 2013
The antioxidant and antidiabetic activity of aerial parts of Aerva sanguinolenta (L) Blume and leaves of Mikania scandens (L.) Willd were investigated by three in vitro and one in vivo assays i.e., by estimating degree of non-enzymatic haemoglobin glycosylation measured colorimetrically at 520 nm; by assaying DPPH free radical scavenging activity; by reduction of phosphomolybdenum complex and by estimating blood glucose levels in alloxan-induced diabetic mice. From the analysis, it was found that the ethyl acetate and chloroform extract of both aerial parts of A. sanguinolenta and leaves of Mikania scandens had higher antioxidant potential than that of other extracts of the plant(s). Among the examined plants, it was also observed that M. scandens L. extracts had better antioxidant and antihyperglycemic action than that of A. sanguinolenta. The antioxidant activity of the extracts was close, identical in magnitude, concentration dependent and comparable to that of standard antioxidant compounds used. Flavonoids and tannins present in the selected extracts may be responsible for such antioxidant and antidiabetic activities.
Journal of Critical Reviews, 2020
Dianthus basuticus is a popular South African medicinal plant used in the management of diabetes mellitus. This study evaluated the antioxidant and antidiabetic potential of D. basuticus using an in-vitro model. The antioxidant activity was determined using iron chelation, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide anion radical scavenging abilities of the aqueous, ethanol and hydroethanol extracts of D. basuticus while the antidiabetic potential was assessed by evaluating the inhibitory effects of the extracts on the activities of a-amylase, a-glucosidase, maltase and sucrase. Aqueous extract displayed significantly higher (p < 0.05) DPPH (2.56 mg/mL) and superoxide radical (7.22 mg/mL) scavenging abilities while ethanol (10.56 mg/mL) and hydro-ethanol (6.95 mg/mL) extracts exhibited strongest hydroxyl radical scavenging and iron chelation activities respectively. The ethanol extract displayed significantly higher (p < 0.05) inhibition of a-amylase (34.02 mg/mL) while aqueous extract exhibited strongest inhibition of a-glucosidase (6.59 mg/mL), maltase (31.21 mg/mL) and sucrase (20.98 mg/mL). Hydro-ethanol and aqueous extract inhibited a-amylase and a-glucosidase in a mixed non-competitive and pure non-competitive manner respectively while the aqueous extract competitively inhibited both maltase and sucrase activities. It can be concluded that D. basuticus extracts possessed antioxidant and antidiabetic activities, and one of its mechanism of antidiabetic action is through the inhibition of diabetes-related enzymes. 2015 Elsevier GmbH. All rights reserved.