The Pilocarpine Model of Acquired Epilepsy (original) (raw)

Elsevier eBooks, 2017

Abstract

Abstract Research in epilepsy has employed a plethora of animal models with which to study and better understand the mechanisms that lead to this debilitating condition. Modeling human disease requires a rigorous approach, and methodologies that can be easily standardized across laboratories. The following chapter will describe the pilocarpine model of acquired epilepsy. The pilocarpine model mimics many of the features of acquired epilepsy in humans, especially that of temporal lobe epilepsy (TLE). A characteristic of the acquired epilepsies is that the onset of seizures is often preceded by an initial precipitating injury, such as stroke, traumatic brain injury, or a prolonged period of status epilepticus (SE). With these types of injuries, the temporal lobe is especially vulnerable. The most prevalent of adult-onset epilepsies, TLE is also known to be difficult to treat and thus the need for novel treatment approaches is great. The following is an in-depth discussion of the post-SE pilocarpine model of acquired epilepsy. Following a brief introduction to TLE, the methods and variables that optimize successful implementation of the pilocarpine procedure itself will be highlighted. How this model can be employed to study both the process of disease progression following SE (epileptogenesis) and/or the chronic disease state will follow the methodological description. Though the pilocarpine model of TLE has existed for nearly 30 years, the development and use of sophisticated monitoring and imaging techniques, in addition to the recent inclusion of various mice strains, warrants an updated review of this model.

Douglas Coulter hasn't uploaded this paper.

Let Douglas know you want this paper to be uploaded.

Ask for this paper to be uploaded.