Supplementary Methods from Antiangiogenic Agent Sunitinib Transiently Increases Tumor Oxygenation and Suppresses Cycling Hypoxia (original) (raw)

Supplemental Data 1 Supplemental Methods Probes for EPRI and MRI Electron Paramagnetic Resonance (EPR) oxygen imaging uses an oxygen-sensitive triarylmethyl radical (TAM) probe OX63 (Suppl. Methods Fig.1), which was obtained from GE Healthcare (London, UK). OX63 is derivatized extensively to confer optimal chemical, pharmacological, and EPR characteristics such as stability, water solubility, low toxicity, long in vivo half-lives, single narrow line resonance, and pO 2-dependent EPR line widths (1). The dose of OX63 used for imaging (1.125 mmol/kg) was well below the maximally tolerated dose of 2.5-7.0 mmol/kg and the LD 50 of 8.0 mmol/kg (2). The pharmacologic half-life of OX63 in kidney and blood is