Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene (original) (raw)
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Journal of Clinical Practice and Research, 2023
This study aimed to assess the relationship between Enterobius vermicularis and appendicitis by determining the frequency of E. vermicularis in appendectomy specimens obtained after a preliminary diagnosis of appendicitis. Materials and Methods: Retrospective evaluation was conducted on the results of appendectomy specimens examined histopathologically at Van Training and Research Hospital Pathology Laboratory from March 2016 to March 2022. Hematoxylin-eosin stained slides from 7,814 appendectomy specimens were scrutinized for the presence of E. vermicularis, acute inflammation, fecaloid material, lymphoid hyperplasia, obliteration, perforation, phlegmonous appendicitis, and peritonitis. Furthermore, slides stained with hematoxylin-eosin (H&E) from patients testing positive for E. vermicularis were re-evaluated by the author in pathology. Results: E. vermicularis was identified in the appendectomy specimens of 50 (0.64%) out of 7,814 patients who underwent appendectomies. In histopathological examinations, the occurrence of lymphoid hyperplasia and fibrous obliteration was higher in E. vermicularis-positive patients compared to those who teste negative. Findings revealed that the mean white blood cell count (WBC) was lower in E. vermicularis-positive patients than in negative patients, while the mean eosinophil percentage (EO%) was higher. Conclusion: These findings imply that although the parasite is not a direct causative factor for appendicitis, it may contribute to the development of factors that result in obstruction in certain cases.
Enterobius vermicularis: A rare Cause of Appendicitis
Turkish Journal of Parasitology, 2012
Objective: Although appendicitis is one of the most common causes of emergency surgery, parasites are rarely found associated with infl ammation of the appendix. The aim of this study is to establish the prevalence of Enterobius vermicularis in surgically removed appendices, as well as to determine its possible role in the pathogenesis of appendicitis. Methods: A retrospective analysis of all the appendices removed during the last 20 years at a tertiary university hospital. Appendices removed during the course of another intra-abdominal procedure were excluded from the study. Results: All 1085 surgical specimens removed from patients with clinical appendicitis were evaluated. Enterobius vermicularis was found in seven appendices (0.65%) with clinical symptoms of appendicitis. The parasite was most frequently identifi ed in appendices without pathological changes (6/117). There was no case of chronic appendicitis presenting E. vermicularis infestation, while the parasite was rarely related to histological changes of acute appendicitis (1/901).
Enterobius vermicularis: A Controversial Cause of Appendicitis
DOAJ (DOAJ: Directory of Open Access Journals), 2004
Enterobiasis is undoubtedly one of the most common human helminths infections through the world, with an estimate of 1000 million cases worldwide. Although adult worms and their eggs are frequently found in inflamed appendices, but the role of the parasite in producing appendicitis in humans still remains controversial. Although acute appendicitis may occur at any age, but it is relatively rare at the extremes of age. A histopathological study of infested appendices with Enterobius vermicularis was performed in Khuzestan province, south western Iran, during 2001 to 2003. All 40 samples had surgically been removed during the emergency operations and histopathological process with (H&E) staining has been performed for each. One of the main objects of this work was to collect more data about the possible role of this highly prevalent human round worm as a causative agent of appendicitis in humans, leading to different types of appendicitis in this part of the country. In this occasion over the slide examinations of the tissue sections, fewer cases than expected, with worm infested appendices were seen exclusively affected with this parasite.
Journal of Parasitic Diseases, 2014
Enterobius vermicularis is one of the most common intestinal parasite in human. The main objective of this study is to determine the role of E. vermicularis in appendicitis through histopathological examination. A cross sectional study included 200 patients who had appendectomy from three hospitals in Gaza strip. The inflamed appendix was the cause of attending the hospital. Histopathological examination for each appendix was carried out. A questionnaire was designed (interview with patients who underwent appendectomy), and information were obtained from patient and analyzed by using SPSS. The study showed that 30 (15.0 %) of 200 appendices had E. vermicularis in histopathological examination. It was found that ages of patients with histologically proven E. vermicularis in appendices less than 18 years old was found to be (18.2 %). Regarding sex, (16.5 %) of females, (14.0 %) of males patients had E. vermicularis in appendices. Patients who had the highest infection with E. vermicularis were students (17.3 %). In conclusion E. vermicularis occurs more frequently inflamed appendices than in normal. From these results we can conclude that E. vermicularis could be associated to cause of appendicitis in Gaza strip.
Journal of Pathology of Nepal, 2015
Background: Appendiceal parasites can cause symptoms of appendicitis. Although the symptomatology imitates acute appendicitis clinically, the true nature of disease is diagnosed through histological examination. The aim of this study is to therefore determine the prevalence of E. Vermicularis in appendicectomy specimens to relate this to acute inflammation histologically.Materials & Methods: Histological data on all appendectomy specimens with a clinical diagnosis of acute appendicitis were retrieved from the archives of department of pathology, B.P.K.I.H.S, over the period of five years (January 2004- December 2008) and was analyzed retrospectively.Results: There were a total of 1528 patients. M:F ratio being 1.2:1.Inflamed appendix constituted for 94.24% of all cases. There were a total of six (0.39%) appendicectomy specimens which showed presence of oxyuriasis appendix. Though all patients with oxyuriasis presented with appendicial colic only one (1) out of the six cases of oxy...
Immunohistopathological Study for Patients with Appendicitis due to Enterobius vermicularis worm
The Egyptian Journal of Hospital Medicine
Background: Intestinal E. vermicularis worm infection is asymptomatic and endemic in Iraq, but it can cause appendicitis; symptoms vary depending on the stage of the gastrointestinal disease. The E. vermicularis worm has caused several cases of appendicitis. Objective: This study aimed to find out how common E. vermicularis was in appendectomy samples and whether it played a role in appendicitis development. Patients and Methods: Just 30 (3.15 %) of the 950 surgical specimens removed from patients with clinical appendicitis tested positive for E. vermicularis worms. Two ml blood was drawn from positive samples to measure cytokines (prostaglandin two and Transforming growth factor-beta 1), with 30 healthy people serving as a control group. From January to August 2019, all samples were taken from patients who visited AL-Zahra maternity and paediatrics and AL-Hakeem hospital in AL-Najaf province. Patients' appendixes were biopsies, stained with eosin hematoxylin, and microscopically diagnosed. Results: The findings of this study showed a substantial increase (P0.05) in serum levels of PGE2 and TGF-1β as compared to the control group, as well as a histological spectrum of appendicitis associated with a large number of E. vermicularis eggs, the lymphoid follicle reactive germinal center, prominent eosinophilic infiltration. Conclusion: Based on this research's findings, we believe that E. vermicularis worm infection can cause appendicitis and an immune response manifested by elevated PGE2 and TGF-1β levels in the blood.
Enterobius Vermicularis Related Acute Appendicitis: A Case Report and Review of the Literature
While the debate on the association between Enterobius vermicularis (E. vermicularis) and acute appendicitis has not been settled, a few case reports of this very rare encounter are beginning to come to light. While E. vermicularis is one of the most common parasitic infestations around the world, acute appendicitis, on the other hand, is also a commonly encountered condition in general surgery. However, the association between the two remains controversial. Here we present a case report of a young woman with appendicitis associated with (E. vermicularis).
BMC Research Notes
Background: Histological acute appendicitis patterns associated with Enterobius vermicularis is an extremely rare finding. The exact role of this parasite in acute appendicitis is controversial as usually resected specimens show no evidence of histological inflammation. Case presentation: We present herein a case of a 21-year-old male Arabic patient who presented with clinical syndrome of acute appendicitis. Emergency appendectomy was performed and the histopathological examination of the resected specimen showed the presence of E. vermicularis as well as intense acute inflammatory patterns such as mucosal ulceration and suppurative necrosis. The post-operative course was uneventful and the patient was discharged with appropriate anti-helmintic drug prescription. Conclusion: Acute appendicitis due to E. vermicularis is a very rare occurrence. The histopathological analysis of resected specimens should pay special attention to search for this parasite for adequate post-operative treatment of patients.
A Study On Entrobius Vermicularis Infection In A Appendices Removed By Surgery In Tabriz Hospitals
The Internet Journal of Parasitic Diseases, 2009
The association of Enterobius vermicularis infestation with acute appendicitis varies from 0.2-41.8% worldwide. Our purpose was to determine the significance of Enterobius-associated appendicitis by retrospective review of appendectomies performed during a 8-year period at a major Tabriz hospital. The Surgical Pathology database at Imam Khomeini's and Alinasab's Hospital, Tabriz,Iran was reviewed for appendiceal specimens found to have Enterobius infestation. Corresponding patient charts were evaluated for age, gender, presenting symptoms, laboratory data, operative findings, and clinical course. Of the 5981 appendectomies performed from 2005 to 2009, 38 specimens (1.38%) were found to contain Enterobius vermicularis. Twentysix of the appendectomies were performed for symptoms of acute appendicitis; the remaining twelve were incidental appendectomies in conjunction with other operations. Between that age groups , and between two age groups of 5-10 and 11-16 the highest rate has been shown that among them females infection is more. In 38 cases there are pinworm that %67 present of it relates to females and %33 of it relates to males. Of the 26 symptomatic children, 12 presented with fever >99.0؛F, and 14 had a WBC count >10,000. Intraoperative appearance of the appendix ranged from normal to perforation. Pathologic evaluation showed neutrophil or eosinophil infiltration in 27 of the 38 specimens. Enterobius infestation is an uncommon cause of acute appendicitis in children in the Islamic Republic of Iran. It may be associated with acute appendicitis, ''chronic appendicitis,'' ruptured appendicitis, or with no significant clinical symptoms.