Antibacterial, Antioxidant and Cytotoxic Studies of the Essential Oil and Ethanol Extract of Aerial Parts of Artemisia rutifolia Steph.ex. Spreng (original) (raw)
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IJRDO-Journal of Applied Science
Mongolia is rich in medicinal plants. Essential oils of aromatic and medicinal plants generally have a diverse range of activities because they possess many active constituents that work through a several modes of action. Artemisia, the largest genus of the family Asteraceae, has a number of effects against human and plant diseases [8].This study was aimed to evaluate antioxidant, cytotoxic activities of aerial parts ethanol extracts from Artemisia scoparia Waldst.et Kit grown in Mongolia. The antioxidant and cytotoxic activities of the essential oil and ethanol extract was determined by using DPPH and MTT assays. The ethanol extracts showed higher antioxidant activity than essential oil. The essential oil of Artemisia scoparia Waldst.et Kit with a concentration of 150 mg/ml or 3μg/disk inhibits the growth of S.enterica 10.6±0.58 mm, B.subtillus 11.6±1.15 mm, and has a moderate bacterial activity.The half-lethal dose (IC50) 56.542 μg/ml is of essential oil of Artemisia sc...
Natural Product Research, 2019
The present study was carried to observed the phytochemical profile of aromatic constituents of Artemisia gmelinni essential oil using GC-FID, GC-MS and 13 C NMR and to evalute the biological activities such as anticancer and antioxidant. Twenty chemical constituents were detected from EO accounting 92.05% of total oil composition. Oxygenated monoterpenes (73.64%) were dominant class of compounds. The major constituents of the EO were isoascaridol (29.70%), alpha-terpinolene (25.37%), phellandrene (9.26%) and ascaridole (4.17%). Ascaridole and isoascaridole are first time identified to be the constituents of this essential oil. The essential oil effectively inhibit the growth of cancer cells and showed maximum anti-proliferative activity at 125 µg/mL concentration in all the three tested cell lines(A-549, MCF-7 and HCT-16), but highest inhibition in cell growth was found in A-549 cell line. Our study revealed that EO was effective in restricting the migration of A-549 cells up to 15% than control at 125 µg/mL concentration. The essential oil also showed moderate antioxidant activity withIC 50 value of 81 ± 2.10 µg/mL and 78 ± 0.001 µg/mL as calculated by hydroxyl radical scavenging assay and DPPH assay respectively.
Digest Journal of Nanomaterials and Biostructures
This study examines the essential oil and the ethanol extract of Artemisia alba Turra herb, in terms of chemical composition, antioxidant and antimicrobial activities in order to select the active pharmacological isolate for making phytopreparation from the group of bitter aromatics and/or bitter liqueurs. Chemical composition of the essential oils and volatile fractions of the extract was determined by GC-FID/GC-MS and non-volatile fractions of the extract by HPLC. The content of total phenolics and flavonoids in the extracts was determined by Folin-Ciocalteu reagent and aluminum chloride, respectively. The antioxidant activity of isolates was tested by FRAP and DPPH tests and the antimicrobial by microdilution method. Major constituents in the essential oil were camphor, artemisia ketone and 1,8-cineole (23.7%, 15.2% and 14.1%, respectively), and in the volatile fraction of the extract, scopoletin (14.0%) and corymbolone (10.3%). The main components in non-volatile fraction of the...
The essential oil of the aerial parts of Artemisia kermanensis was analyzed by GC-FID and GC-MS methods. Twenty-five compounds, accounting for 92.3% of the total oil, were identified. The main components of the oil were isoborneol (21.5%) and camphor (9.8%). The oil was found to be rich in regards to oxygenated monoterpenes (80.2%). The antimicrobial activity of oil was determined against eight bacteria and two fungal strains. The results of inhibition zone showed that this oil was active against some of the tested strains. As the results, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus were the most sensitive microorganisms to the oil (having MIC value 1.25 mg/ml). The oil antioxidant activities were measured by DPPH assay and ß-carotene-linoleic acid tests. The essential oil significantly reduced the concentration of DPPH free radical (71.6%), with an efficacy higher than that of trolox (48.1%). Also, a 50% inhibition was noted in formation of peroxidation products in ß-carotene bleaching test. The activity of the oil may be due to the presence of phenolic and major compounds.
International Journal of Biosciences | IJB |, 2022
The main objective of this study is to investigate the chemical composition, antioxidant and antimicrobial activities of the essential oil of Artemisia herba-alba Asso. of southern Algeria. Essential oil of Artemisia herbaalba Asso was extracted by hydrodistillation, and their chemical composition was identified by GC/MS Antioxidant activity of essential oil, has been done by using DPPH assay. The antimicrobial activity of essential oil was realised by the agar disc diffusion method. The essential oil extracted from the aerial parts by hydrodistillation was analysed by GC/MS. 39 constituents, representing 99.3% of the oil, were identified, of which the major ones, Thujone (12,759 %), Camphor (7,751%), Eacalyptol (4,525%), Isoborneol (1,119%). IC50 values observed for DPPH essay were 20,27 ±0,767 mg/ml. On the other hand, this oil was found effective against all tested strains; this activity ranged from 15.67±1.53 mm with Listeria innocua CIP 74915. These results provided evidence that the studied plant might indeed be a potential source of natural antioxidant and antimicrobial agents.
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2021
Essential (volatile) oil from leaves of Artemisia monosperma L. belonging to family Asteraceae, and aerial parts of Tamarix aphylla L. (Athel) belonging to family Tamaricaceae were collected from the desert of Ha'il region, northern region of Saudi Arabia, hydro distilled by Clevenger apparatus and analysed by means of GC-MS techniques. Antioxidant activities of essential oils of A. monosperma and T. aphylla compared with ascorbic acid and butylated hydroxytoluene (BHT) as reference antioxidant compound were determined by method of DPPH radical scavenging assay and ABTS assay. In vitro screening of potential cytotoxicity of essential oils was also evaluated against human promyelocytic leukaemia cell lines (HL60 and NB4). The GC/MS analysis of A. monosperma essential oil resulted in identification of 61 components predominated mainly by β-Pinene as principal component (29.87%) and T. aphylla resulted in identification of 37 components of essential oil predominated mainly by 6,10,...
Industrial Crops and Products, 2012
Hydrodistilled volatile oil obtained from the aerial parts of Artemisia annua L., cultivated near Sarajevo, Bosnia, was analyzed by GC–MS. More than one hundred compounds were identified, representing 95.5% of the total oil. The major constituents of essential oil were oxygenated monoterpenes, artemisia ketone (30.7%) and camphor (15.8%). Isolated essential oil was tested for radical-scavenging ability using the stable DPPH radical, the ABTS radical, for reducing power ability with a test based on the reduction of ferric cations, for reducing ability of hydroxy radical in ORAC assay, and for metal chelating ability using the ferrozine assay. In all tests oil did not show a prominent antioxidant activity, but still comparable with thymol, an already known antioxidant. The screening of antimicrobial activity of oil was individually evaluated against representatives of Gram-positive, Gram-negative bacteria and fungi, using the agar diffusion method. All tested microorganisms were inhibi...
The Journal of Pharmacy
In the present study, the essential oil components of the leaf, stem and roots of Artemisia dracunculus have been analyzed using a combination of capillary GC-FID and GC-MS analytical techniques. The study led to the identification of 17 components in the leaf and 13 in root essential oils accounting for 85.5% and 91.2% respectively of the total oil composition. In the stem essential oil, 24 components accounting for 94.6% of the total oil were identified. The principal components identified in the leaf essential oil were (z)-ß-ocimene (12.2%), capillene (12.6%), acenaphthene (51.7%) and elemicin (2.7%). In the stem, the major components were (z)-ß-ocimene (17.6%), capillene (34.7%) and acenaphthene (32.6%) while as in the root the major components were capillene (22.8%) and acenaphthene (66.6%). The aromatic hydrocarbon acenaphthene is reported for the first time in tarragon essential oil growing in this geographical part of the world.
GC-MS analysis and antioxidant activities of essential oils of two cultivated Artemisia species
Chemistry of Natural Compounds, 2007
Artemisia is a fairly large genus within the family Asteraceae (alt. Compositae), with ca. 300 individual species of aromatic and medicinal plants, which are usually found as small fragrant shrubs or herbs in the northern hemisphere [1, 2]. While some of their essential oils are used in perfumery and medicine, the leaves of some species are used as culinary herbs [3]. The various pharmacological effects of Artemisia species, e.g., anticonvulsant [4], apoptosis [5], immunosuppressive [6], antipeptic ulcer [7], anthelmintic [8], antimalaria [9], anti-inflammatory [10], anti-HIV, antiplatelet aggregation [11], antibacterial [12] and antifungal effects [13], have previously been reported. We now report on the determination of the main constituents of the essential oils from two cultivated Artemisia species, Artemisia fragrans and A. austriaca, aiming at the comparison between the composition of the oils from these cultivated species with those of wild species. The free radical scavenging properties (antioxidant) of these essential oils using the DPPH assay are also reported. The aerial parts of A. fragrans Willd. and A. austriaca Jacq., cultivated in the Botanical Garden of the Drug Applied Research Center (Tabriz University of Medical Sciences, Tabriz, Iran), were collected during August-September 2004 (flowering). The steam distillation for 3 h of the aerial parts of two cultivated species, Artemisia fragrans and A. austriaca, yielded essential oils 0.95% and 1.1%, respectively. The percentage yield of the essential oil of A. austriaca was more than that of A. fragrans. While the total yield of essential oils of the cultivated A. fragrans was similar to that of this species growing wild (0.97%) [2], the yield was much higher, almost doubled, in the cultivated A. austriaca than its wild variety (0.59%) [14]. The identified constituents of the oils of A. fragrans and A. austriaca are listed in Table 1. The GC-MS analysis of the aerial parts of A. fragrans and A. austriaca led to the identification and quantification of a total of 14 main compounds (Table 1), and these compounds accounted for the 88.7% and 91.5% of the total components present, respectively. Camphor (2), which has a bornane skeleton, and 1,8-cineole (7) are the main components of the essential oils of Artemisia species [2, 14]. According to a previously published reports [14, 15], the main components of the essential oil of A. austriaca were camphor (2, 45.5%), 1,8-cineole (7, 30.4%), camphene (1, 6.5%), α-terpineol (3.2%), α-pinene (3.0%), terpinen-4-ol (8, 2.9%), and p-cymene, and those of the essential oil of A. fragrans were camphor (2, 46.0%), 1,8-cineole (7, 23.7%), camphene (1, 7.9%), borneol (4.9%), and germacrene D (14, 1.9%) [2, 16, 17]. All these previously published reports were on the species that were collected wild. A clear variation in the profile as well as in the amounts of individual compounds present in the cultivated species (Table 1) in the present study was identified when compared with wild species. Both in the cultivated A. fragrans and A. asutriaca, the two major components were camphor (2, 54.92% and 40.59%, respectively) and 1,8-cineole (7, 11.48% and 27.97%, respectively), which accounted for ~70% of the total essential oils (Table 1). In addition to 2 and 7, while in the cultivated A. fragrans, α-thujone (11, 9.21%), β-thujone (12, 4.83%), and germacrene D (14, 3.57%) were identified in significant amounts, camphene (1, 3.95%) and trans-pinocarveol (6, 2.69%) were present in significant proportions in the cultivated A. austriaca.