How the ribosome moves along the mRNA during protein synthesis (original) (raw)

The study investigates the mechanics of ribosome movement along mRNA during protein synthesis. Using mRNA labeled at one end and fixed to ribosomes with specific tRNAs, findings reveal that the ribosome traverses the mRNA without moving at the A-site, exhibiting simultaneous translocation on both sides. Key experiments confirm the dimensions of mRNA interactions and challenge previous consensus on the length of protected mRNA segments, suggesting dynamic interactions during translation elongation.