High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination (original) (raw)

Quantitative phase microscopy (QPM) is challenged by coherent noise and limited spatial bandwidth when using highly coherent light sources like lasers. This paper presents a method to achieve high-throughput spatial sensitivity in QPM by utilizing low spatially coherent light sources, such as white light or LEDs, combined with coherence plane tilting techniques. The results demonstrate significant improvements in spatial phase sensitivity and field of view, enabling better analysis of live cell dynamics while addressing issues related to coherent noise and acquisition speed.