Production and potential applications of Egg Yolk antibodies (Igy) as anti-bacterial prophylactic uses for infectious diseases (original) (raw)
Related papers
Atla-alternatives To Laboratory Animals, 2005
The production of antibodies (Abs) in chickens and the extraction of specific Abs from egg yolk (IgY Abs) are increasingly attracting the interest of the scientific community, as demonstrated by the significant growth of the IgY literature. This review offers detailed and comprehensive information about IgY-technology, including: a) possibilities for hen keeping in accordance with the Three Rs principles; b) new insights into the IgY transfer mechanism from blood to yolk as a biological basis for the technology; c) the comparative characteristics of IgY Abs and IgG Abs; d) the high efficacy of the technique, in view of the extraordinary amount of IgY Ab produced by one hen in one year (between 20g and 40g IgY in total); e) comparisons between the efficacies of IgY Abs and IgG Abs (rabbit, sheep, mouse) in several immunological assays; f) immunisation protocols, as well as the most commonly used IgY-extraction procedures; g) new possibilities for application in human and veterinary medicine, including strategies for the treatment of Helicobacter pylori infection or fatal intestinal diseases in children, particularly in poor countries, for reducing the use of antibiotics, and, in Asia and South America, for producing Abs against snake, spider and scorpion venoms; and h) the use of IgY Abs in various fields of research, also taking into consideration recent developments in South America (particularly Argentina and Cuba) and in Asia.
2009
Enteric infections caused by Salmonella remain a major public health burden worldwide. Poultry, particularly chickens, are known to be the main reservoir for this zoonotic pathogen. Therefore, the prevention and monitoring of Salmonella infection during the live phase may greatly reduce the contamination of poultry meat during slaughter and processing. With the ban on sub-therapeutic antibiotic usage in Europe and the increasingly strictness of the European legislation on food hygiene, passive immunization by oral administration of pathogen-specific hen egg yolk antibody (IgY) may be a useful and attractive alternative. This review offers summarized information about IgY production and the use of these antibodies for passive immunization, particularly in poultry. Keywords. Hen egg yolk antibodies, IgY production, passive immunization, enteric infections, feed additive. Les anticorps du jaune d'oeuf de poule (IgY), production et utilisation en immunisation passive contre les infections entériques bactériennes : une revue. Les infections entériques causées par Salmonella constituent un problème majeur de santé publique à travers le monde. Il est bien connu que la volaille, en particulier le poulet de chair, constitue le principal réservoir pour ce pathogène zoonotique. Par conséquent, la prévention et la surveillance de Salmonella au cours de la phase d'élevage pourrait réduire efficacement la contamination de la viande à l'abattoir et lors de la transformation. Avec l'interdiction de l'utilisation sub-thérapeutique des antibiotiques en Europe et la rigueur croissante de la législation européenne en matière d'hygiène alimentaire, l'immunisation passive par l'administration orale d'anticorps de jaune d'oeuf (IgY) spécifiques du pathogène serait une alternative intéressante. Cette synthèse bibliographique donne des informations concises sur la production des IgY et leur utilisation en immunisation passive, en particulier chez la volaille. Mots clés. Anticorps du jaune d'oeuf, production de l'IgY, immunisation passive, infections entériques, additif alimentaire.
Antibodies have become essential tools in recent decades, with a wide range of applications in the laboratory and in human and veterinary medicine. The use of laying hens, instead of mammals, to obtain the necessary antibodies from the eggs is a major advance in terms of animal welfare because it makes blood sampling obsolete. However, the advantages of this technology are numerous, in addition to the animal welfare aspect. With a carefully designed immunization protocol, it is possible to enhance both the hen's immune response and its welfare during the process. This review puts forward recommendations how to do this and discusses recent approaches on improving the technology.
The Use of Egg Yolk Antibodies for Food Protection and Immunity
DergiPark (Istanbul University), 2020
The chicken immune system has been studied for many years. These studies have contributed substantially to our understanding of the fundamental concepts of immunology and the development of different immunoglobulin classes. It is thus surprising that only a small fraction of the antibodies presently used in laboratories are of avian origin. Laying hen produces more yolk antibodies than rabbit at the same time. Animal care costs are lower in chickens compared to rabbits. Chicken antibodies offer many advantages to the traditional mammalian antibodies when used for the detection of mammalian antigen. Chicken antibodies can also be used to avoid interference in immunological assays caused by the human complement system, rheumatoid factors, human anti-mouse IgG antibodies or human and bacterial Fc-receptors.
Purification of egg yolk immunoglobulins
Journal of Immunological Methods, 1988
A two-step chromatographic procedure was developed for the isolation and purification of hen IgY antibodies from egg yolk. The antibodies were completely separated from vitellin and lipids by hydrophobic interaction chromatography followed by gel filtration. Almost no residual yolk proteins, no immunoglobulin aggregates, and no antibody fragments could be detected in the final extract. Moreover, the method described, guarantees the recovery of antibodies of undiminished activity. Although the final yield is somewhat lower than that obtained by an isolation method consisting of two precipitation steps with polyethylene glycol and alcohol respectively, the procedure described is particularly recommended when highly purified antibody preparations are needed.
Production and purification of IgY antibodies from chicken egg yolk
Journal of Genetic Engineering and Biotechnology, 2018
The isolation of polyclonal antibodies from the serum of immunized mammals has significantly contributed to scientific research and diagnosis. The fact that recent technologies allow the production of antibodies in the yolk of eggs laid by hens, has led to the development of an alternative method for antibody generation that is less stressful to animals. As hens are kept under almost all their natural conditions, antibodies are isolated from the collected eggs; this technology is expected to become an interesting alternative to the conventionally serum-based techniques that eventually require to sacrifice the animal. Here we present a modified protocol for the isolation of IgY antibodies from immunized chickens and provide comparison between two chicken breeds in relative to IgY yield per egg. Our results show the possibility of generating large quantities of highly pure IgY from chicken eggs and also show large differences in the yield of IgY production between the two studied breeds. The results of this work indicate that IgY technology can be used for the production of primary antibodies for immunological work and disease diagnosis.
The Production of Avian (Egg Yolk) Antibodies: IgY
Alternatives to Laboratory Animals, 1996
for the Validation of Alternative Methods. 2 This document represents the agreed report of the participants as individual scientists. 926 22-24 March 1996, under the chairmanship of Christian Staak (BgVV, Berlin, Germany). The participants at the workshop, who were representatives from academia, industry and government, reviewed the current status of immunoglobulin Y production in the chicken. Particular emphasis was placed on discussion of: a) the housing of chickens; b) immunisation protocols; c) egg-laying behaviour; d) the isolation and purification of lgY; and e) the successful replacement of the use of mammalian antibodies by IgY. The main issues discussed and recommendations made in connection with these topics are summarised in this report.
A novel isolation method for hen egg yolk antibody,IgY
Agric. Biol. Chem, 1990
A method for isolation of egg yolk immunoglobulin, IgG, a livetin protein, was investigated. Several natural gums (carrageenan and xanthan gum) were found to be effective for removal of yolk lipoprotein as a precipitate. The effect was pronounced with /-carrageenan and the lipid content in the supernatant after removal of the resulting precipitate was less than 0.4% of that of egg yolk. IgY remained in this supernatant, with a yield of 86%, and was isolated by chromatography on a column of DEAE-Sephacel followed by salting-out with sodium sulfate. IgY thus isolated was almost pure (98%) and the yield was 70 to 100mg per egg.
Eggs: Conveniently packaged antibodies. Methods for purification of yolk IgG
Journal of Immunological Methods, 1981
Eggs from immunized chickens may provide a convenient and inexpensive source of antibodies. We describe two simple and efficient methods for purification of IgG from yolk. The antibody is rendered useful for most currently employed immunological techniques. Amounts of antibody corresponding to almost half a litre of antiserum may be recovered from a chicken in one month.