Rapid and Quantitative Detection of Malarial Antigen for Microfluidic Point-Of-Care Diagnosis in the Developing World (original) (raw)
2008, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
A novel, point-of-care immunoassay format is demonstrated for malarial diagnosis. The assay system consists of a disposable card with integrated dry reagent storage and an external reader that conducts fluidic actuation and assay quantification. Reagents flow through a porous membrane encased in the card's polymeric laminates to conduct a colorimetric sandwich assay on the membrane's surface. The system produces quantitative results with detection limits on the order of ELISA in under 9 minutes.
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