Bioprocessing, biochemical characterisation and optimization of solid state fermentation of a new thermostable xylanase producing strain belonging to Bacillus genus (original) (raw)
The present study was aimed at isolation and characterization of a new xylan degrading strain of Bacillus from soil for production of xylanase. Different soil samples from Punjab state were used to isolate xylan degrading strains. We isolated a highly active xylanolytic strain and performed genetic characterisation using 16S r-RNA analysis. DNA sequencing and phylogenetic analysis using MEGA4 software showed our strain to be a novel strain (accession number JQ916900) which was named Bacillus lpuarvinder st. lpu002. Biochemical characterization of the enzyme showed optimum activity at 60 o C and pH 8.6. The enzyme was stable at 70 o C for up to 6 hours. The xylanase activity seems to be alleviated by divalent Ca 2+ , Mn 2+ and Mg 2+ ions. The best enzyme activity was obtained when the cells were grown in Bushnell-Haas medium supplemented with 0.5% xylan and beef extract. The process optimization was done using solid state fermentation (SSF) using wheat straw as substrate. The enzyme ...
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