Antibodies against the SV40 large T antigen nuclear localization sequence (original) (raw)

Transport of large proteins into the nucleus necessitates a nuclear localization signal (NLS) and its interaction with transport machinery. Polyclonal and monoclonal antibodies against the NLS of SV40 large T antigen were generated, successfully recognizing T antigen and exhibiting binding inhibition by NLS peptides. The study found that while NLS-specific antibodies remained cytoplasmic, antibodies against other epitopes of T antigen were co-transported into the nucleus, indicating a requirement for NLS exposure in nuclear transport.