Molecular Characterization of Rotavirus Strains Circulating in Oman in 2005 (original) (raw)
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Journal of Clinical Microbiology, 2008
In anticipation of rotavirus vaccine introduction in Saudi Arabia, this study was undertaken to determine the distribution of the G and P genotypes of rotaviruses in order to examine whether there was any emerging serotype or unusual strain circulating in children in Saudi Arabia. Of 984 stool specimens collected between 17 April 2004 and 16 April 2005, rotavirus was detected by an enzyme-linked immunosorbent assay in 187 (19%) diarrheal children less than 5 years of age. Of these, 160 (86%) were classified into G and P genotypes as follows: G1P[8] (44%), G2P[4] (20%), G9P[8] (11%), G12P[8] (4%), and G3P[8] (4%). RNA polyacrylamide gel electrophoresis identified 94 (50%) specimens as long RNA patterns, 30 (16%) specimens as short RNA patterns, and 1 mixed infection. Only a single long RNA electropherotype was identified for seven specimens containing G12P[8] rotavirus. RNA-RNA hybridization demonstrated that the G12P[8] strains were similar in their genomic constellation to locally ...
Molecular Epidemiology of Rotavirus Strains Circulating among Children with Gastroenteritis in Iran
Rotavirus diarrhea is a leading cause of child death. It is a major concern in developing and developed countries. In Iraq, few studies and analysis were executed for genetic assessment of rotavirus.The aim of this study was to estimate the prevalence of rotavirus caused gastroenteritis and identify the distribution of the circulating G and P types of rotavirus among infants admitted to the hospital or treated as outpatients in three governorates in the region of Mid-Iraq for symptoms of gastroenteritis.This study was undertaken during the period from June 2010 to April 2012 in infants under 1 year suffering from acute gastroenteritis. Group A rotaviruses were detected in stools by latex agglutination test and genotyped using semi nested multiplex reverse transcription PCRs with type-specific primers on the basis of their outer capsid proteins.Of the 348 stool specimens from infants with acute gastroenteritis, 42.45% were positive for rotavirus A. The predominant G type was G1 (48.57%), followed by G2 (22.14%), G9 (11.42%), G3 (2.14%), G4 (0.71%). Only P [8] (61.4%), P [4] (11.4%) and P [6] (5.7%) genotypes were found. Infants of 9 and 10 months of age were most frequently affected. The prevalence of rotavirus infection peaked in the Autumn season, when temperatures were low, and decreased in summer. Epidemiological knowledge of rotavirus is critical for the development of effective preventive measures, including vaccines. These data will help to make informed decisions as to whether rotavirus vaccine should be considered for inclusion in Iraqi National Immunization Program.
Genetic and antigenic analysis of human rotavirus prevalent in Al-Taif, Saudi Arabia
Journal of Tropical Pediatrics, 1996
The subgroup, serotype and electropherotype diversity of human rotavfrns strains was investigated in Al-Taif, Saudi Arabia. Out of 349 faecal samples collected from diarrbodc children, 150 (43 per cent) tested rotarirns positive by a gronp-A specific enzyme-linked imnmnosorbent assay (ELISA). The majority (87 per cent) of the infected children were below 2 years of age. Snbgronping and serotyping of rotavimses with specific monoclonal antibodies showed that of the 150 rotavfa-ns positive specimens, 17 per cent belonged to subgroup I, 59 per cent belonged to subgroup II, and 24 per cent were neither subgroup I nor subgroup n. The specimens were typed, as serotype 1 (43 per cent), serotype 2 (5 per cent), serotype 3 (11 per cent), serotype 4 (10 per cent) or mixed serotypes (3 per cent). The remaining 41 (27 per cent) specimens were untypeable. None of the serotypes showed association with a particular age group. An electrophoretic analysis of viral RNA revealed 11 distinct patterns (six long ind five short). The majority, 78 per cent were long patterns and 22 per cent were short patterns. Analysis of the specimens for which subgroups, serotypes and electropberotypes were available indicated that a given RNA pattern does not correspond to a particular subgroup or serotype.
BMC Infectious Diseases, 2011
Background: Rotavirus infection is the most common cause of severe, dehydrating, gastroenteritis among children worldwide. In developing countries, approximately 1440 children die from rotavirus infections each day, with an estimated 527,000 annually. In infants, rotavirus is estimated to cause more than 2 million hospitalizations every year depending on the income level of the country. The purpose of this study was to estimate the proportion of rotavirus gastroenteritis and identify the distribution of circulating G and P genotype rotavirus strains among children consulting several dispensaries in the region of Monastir (outpatients departments) or admitted to Monastir University Hospital (inpatients department) with acute gastroenteritis. Methods: This study was undertaken during a 3-year period from April 2007 to April 2010 in Tunisian children under 13 suffering from acute gastroenteritis. Group A rotaviruses were detected in stools by ELISA and genotyped using multiplex reverse transcription PCRs with type-specific primers on the basis of their outer capsid proteins. Statistical analyses were performed with SPSS software, version 19. Results: Of the 435 stool samples from children with acute gastroenteritis, 27.6% were positive for rotavirus A. The predominant G type was G1 (37.5%), followed by G3 (25%), G2 (17.5%), G4 (12.5%), G9 (2.5%) and three mixed-G infections G3G4 (2.5%) were identified. Only P[8] (80.8%), P[4] (16.7%) and P[9] (0.8%) genotypes were found. The predominant single G/P combination was G1P[8] (37.5%), followed by G3P[8] (25%), G2P[4] (16.7%), G4P[8] (12.5%), G9P[8] (1.7%) and one case of the unusual combination G9P[9] (0.8%). The G-mixed types G3G4 combined with P[8] (2.5%). Infants less than 3 months of age were most frequently affected. The prevalence of rotavirus infection peaked in the winter season, when temperatures were low, and decreased in summer. Conclusions: Rotavirus gastroenteritis is a common disease associated with significant morbidity, mortality, and economic burden. Epidemiological knowledge of rotavirus is critical for the development of effective preventive measures, including vaccines. These data will help to make informed decisions as to whether rotavirus vaccine should be considered for inclusion in Tunisia's National Immunisation Programme.
Infection, Genetics and Evolution, 2017
Hospital-based surveillance of acute gastroenteritis caused by rotavirus has produced ample knowledge on the infection in children, whereas little is known on rotavirus infection among adults. The Italian surveillance program RotaNet-Italia collected 1,595 samples from patients admitted to hospital with gastroenteritis in 2012. All patients presented with vomiting, diarrhea, fever, and/or abdominal pain. Fortytwo samples obtained by the RotaNet-Italia (2.6%) were from adolescents or adults (10-89 years). The study compared the genotypes and gene sequences of the rotavirus strains identified in adults with strains obtained from children worldwide. All 42 Italian strains were genotyped by the EuroRotaNet RT-nested-PCR protocols, and 12 rotaviruses from patients >13-year-old were subjected to nucleotide sequencing of their VP7 and/or VP4 genes. All strains analyzed belonged to the common human genotypes G1P[8], G2P[4], G4P[8], and G9P[8], except an uncommon G3P[19] genotype detected in a single patient. Phylogenetic analysis of the 12 strains showed that within each genotype they clustered in RVA lineages reported worldwide. The amino acid sequences of the VP7 and the VP8 Ã hypervariable regions were highly conserved between the RVA strains collected from adults and children, in each lineage. Genotyping, phylogenetic analysis, and the study of viral epitopes revealed that rotaviruses circulating in adults in Italy are closely similar to the strains circulating in children, with high nucleotide identity particularly with strains reported in Europe and Asia. The circulation of the same rotavirus strains in both children and adults suggests that adults may contribute to sustain the circulation of rotaviruses through the population.
Unusual rotavirus genotypes among children with acute diarrhea in Saudi Arabia
BMC infectious diseases, 2015
Human rotavirus A (human RV-A) is the most common cause of viral gastroenteritis in infants. The objective of the study was to characterize the G and P genotypes among clinical rotavirus isolates from children with acute diarrhea admitted to a tertiary care hospital in Riyadh, Saudi Arabia. From 2011 to 2012, 541 pediatric patients with acute diarrhea were tested for rotavirus infection. RNA extractions from the fecal specimens were done by commercial kit. RT-PCR and sequencing techniques were used to detect the prevalent genotypes. Phylogenetic analysis by Maximum Likelihood method was used to study the clustering of the circulating genotypes. The data showed that 171/541 (31.6%) faecal samples were positive for human RVA and majority were children aged below 2 years. From the G and P [types] detected it was seen that (a) 171 minus 43 ie. 128 rotavirus positives were G typed successfully (b) 171 minus 20 ie. 151 rotavirus positives were P typed successfully; (c) overall G [P] natur...
Saudi journal of biomedical research, 2020
Diarrhea remains the first leading causes of children mortality worldwide specially in developing countries, lead to approximately (1.87) million deaths yearly.There is a global agreement that rotaviruses are the single prominent causative agents of childhood diarrhea illnesses worldwide. The objective of this work was to characterize human rotavirus strains circulating among children attended with diarrhea to Mohammed Alamin Hamid Pediatric Hospital using molecular methods. 150 fecal specimens from infants less than 5 years were collected and analyzed. The samples were investigated for presence of rotavirus using antigen based enzyme immune-sorbent assay (ELISA), genotyping was carried out by RT-PCR to determine virus genotypes using VP7 gene specific primer sets. Rotavirus was detected in stool of 42(28.7%) out of 150 total children. The study revealed that the percentage of (Rotavirus Ag positive) children was (29.3%) in males which is nearly equal to the percentage of Rotavirus Ag positive children in females (26.6%) but this difference in gender was significant statistically insignificant.
International Journal of Multidisciplinary Research and Development, 2021
Background: Rotavirus is a much more popular cause of viral diarrhea. and it has several genotypes. Objectives: The aim of this study is to molecular identification of the Rotavirus G and P genotypes by detecting the VP7 and VP4 genes and to evaluate the relationship between the clinical presentation and rotavirus genotypes. Material and Methods: This study was conducted on children with diarrhea under (5) years at Batool Hospital in Diyala province during 2019-2020. RNA extraction of 142 stool samples for children with rotavirus virus and G-P genotypes identified by Multiplex semi-nested RT-PCR.