The significance of minor components on the antibacterial activity of essential oil via chemometrics (original) (raw)
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Journal of Medicinal Plants Research, 2012
In this study, the chemical composition of essential oils isolated from dried stems, leaves, and fruits of three wild sages from Northern Iran (Artemisia absinthium L., Artemisia annua L., and Artemisia tschernieviana B) were investigated by a combination of gas chromatography (GC) and gas chromatography (GC)/mass spectrometry (MS). A total of 83 components were identified accounting for 93.51 to 100.00% of the oil composition. A. absinthium oils were characterized by high amounts of βthujone (24.27 to 40.91%), 1,8-cineole (8.10 to 14.09%), and sabinene(8.35 to 10.05%). A. annua yielded an oils rich in camphor (10.54 to 24.12%), borneol (1.48 to 27.78%), and 1,8-cineole (8.03 to 11.71%). βpinene (13.65 to 22.37%), limonene (7.69 to 13.65%), and cubenol (3.50 to 15.43%) were the main components in the essential oils of A. tschernieviana. The total numbers of volatile compounds identified from A. absinthium, A. annua, and A. tschernieviana were 58, 84, and 86, respectively. In A. absinthium and A. annua, monoterpens were higher than the sesquiterpens, and oxygenated monoterpene compounds were the main constituents, but in oils of A. tschernieviana, monoterpene hydrocarbons were the main constituents. The results of the antimicrobial activity of all essential oils showed that the oils had varying degrees of growth inhibition against the microorganisms tested.
2012
The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and β-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.
Journal of Microbial & Biochemical Technology, 2014
Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in treatment of antibiotic-resistant bacteria. The present study aimed to evaluate the efficacy of essential oil extracted from Achillea fragrantissima plant growing in Egypt for antimicrobial, antiviral activities and chemical composition analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). We also performed determination of essential oil antimicrobial activity by agar desk diffusion method and Minimal Inhibitory Concentration (MIC). Also, antiviral activity on ORF virus by pock reduction test was performed. It was reduced virus titer from 5.9 to 1.00 at 180 minutes. Detection of beta lactams resistant bacteria (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli) by PCR with primers of mecA gene and Bela gene. The essential oil obtained by hydrodistillation was analyzed by GC-MS. The major components were found to be Santolina triene (1.97%), 2,5,5-trimethyl-3,6-heptadien-2-ol (8.23%) Eucalyptol 8.17, trans-2,7-Dimethyl-4,6octadien-2-ol (24.40%), 1,5-Heptadien-4-one-3,6-trimethyl (7.65%), Artemisia alcohol (3.49%), à Thujone (33.97%), Cissabinol (1.92%), Lavandulol (0.71%), 2-Octen-4-ol, 2-methyl (2.02%), 3-Cyclohexen1ol,4-methyl1 (1 methylethyl) (CAS) (2.15%), à terpineol (0.05%), Estragole (0.71%) Lavandulyl acetate (0.49%), Sabinyl acetate (2.12 %), Germacrened (0.94%). Finally, our study proved that the essential oil has bactericidal effect on some bacterial resistant antibiotic (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli) as well as antiviral activity on ORF virus but it is still need further extensive studies for safety and drug interaction.
Flavour and Fragrance Journal, 2019
The chemical composition of essential oils (EOs) distilled from three wild Artemisia species, Artemisia herba‐alba, Artemisia judaica, and Artemisia monosperma, collected from Egypt, and Saudi Arabia, were analysed by gas chromatography–mass spectrometry (GC‐MS). The antibacterial activities of the EOs for all species were determined using the Agar disc diffusion method. The yield of the EOs ranged from 0.16 to 0.49%. The results showed that A. monosperma plants growing in Saudi Arabia gave the highest yield, compared with A. herba‐alba, A. judaica, and A. monosperma collected from Egypt. In total, 92 volatile compounds were identified from the aerial parts of the plants using GC‐MS. Oxygenated monoterpenes were the major class in A. herba‐alba (75.31%) and A. judaica (83.07%), whereas monoterpene hydrocarbons were the major representative group in A. monosperma collected from both Saudi Arabia (34.23%) and Egypt (36.23%). Yomogi alcohol (22.4%), artemesia alcohol (9.91%), and 1,8‐c...
Arabian Journal of Chemistry, 2020
The present study describes the chemical composition and antimicrobial activity of essential oil from Salvia potentillifolia Boiss. & Heldr. ex Benth., which is endemic for Turkey. The essential oil was obtained by hydrodistillation and components of the essential oil were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The major components of S. potentillifolia essential oil were 1.8-cineole (19.33%), -pinene (11.97%) and -pinene (8.99%). Antimicrobial activity of the essential oil was investigated against standard control and resistant strains, using disc diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. The essential oil of S. potentillifolia showed relatively low levels of antimicrobial activity against the bacteria tested. However, the oil was as effective as the antibiotic tested against Staphylococcus aureus ATCC 29213, Staphylococcus aureus ATCC 43300 [methicillin resistant Staphylococcus aureus (MRSA)], Enterococcus faecalis ATCC 51299 [vancomycin resistant Enterococcus faecalis (VRE)], Enterococcus faecalis ATCC 29212, Haemophilus influenzae ATCC 49247 strains. The most bactericidal activity was obtained against Haemophilus species (4 μg/ml).
Phytochemistry, 2008
The chemical composition of essential oils isolated from aerial parts of seven wild sages from Western Canada -Artemisia absinthium L., Artemisia biennis Willd., Artemisia cana Pursh, Artemisia dracunculus L., Artemisia frigida Willd., Artemisia longifolia Nutt. and Artemisia ludoviciana Nutt., was investigated by GC-MS. A total of 110 components were identified accounting for 71.0-98.8% of the oil composition. High contents of 1,8-cineole (21.5-27.6%) and camphor (15.9-37.3%) were found in Artemisia cana, A. frigida, A. longifolia and A. ludoviciana oils. The oil of A. ludoviciana was also characterized by a high content of oxygenated sesquiterpenes with a 5-ethenyltetrahydro-5-methyl-2-furanyl moiety, of which davanone (11.5%) was the main component identified. A. absinthium oil was characterized by high amounts of myrcene (10.8%), trans-thujone (10.1%) and trans-sabinyl acetate (26.4%). A. biennis yielded an oil rich in (Z)beta-ocimene (34.7%), (E)-beta-farnesene (40.0%) and the acetylenes (11.0%) (Z)-and (E)-en-yn-dicycloethers. A. dracunculus oil contained predominantly phenylpropanoids such as methyl chavicol (16.2%) and methyl eugenol (35.8%). Artemisia oils had inhibitory effects on the growth of bacteria (Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis), yeasts (Candida albicans, Cryptococcus neoformans), dermatophytes (Trichophyton rubrum, Microsporum canis, and Microsporum gypseum), Fonsecaea pedrosoi and Aspergillus niger. A. biennis oil was the most active against dermatophytes, Cryptococcus neoformans, Fonsecaea pedrosoi and Aspergillus niger, and A. absinthium oil the most active against Staphylococcus strains. In addition, antioxidant (beta-carotene/linoleate model) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were determined, and weak activities were found for these oils.
AFRICAN JOURNAL OF BIOTECHNOLOGY
The antimicrobial activities of 10 essential oils extracted from various plant species were investigated and compared with the activities of 10 commercial antibiotics against 10 strains of bacteria using agar diffusion method. Although, all the essential oils were active at concentration ranging from 0.5 to 1.5 mg/ml, their activities were more lower than the commercial antibiotics. However, being natural products the oils have been reported to be much safer than the antibiotics. Another advantage of the essential oils used in this study was their broad spectrum activities against gram positive and gram negative bacteria. The oils were analyzed by GC and GC-MS techniques in order to determine their active compounds.
Chemical Composition and Antibacterial Potential of Artemisia arborescens L. Essential Oil
Current Microbiology, 2010
This study was undertaken to characterize the essential oil (EO) of Artemisia arborescens growing wild in Sicily. EO, extracted by steam distillation, was examined for its chemical composition and for its capability to inhibit some food-borne pathogen bacteria. A total of 43 compounds (13 monoterpene hydrocarbons, 14 oxygenated monoterpenes, 10 sesquiterpene hydrocarbons, three oxygenated sesquiterpenes and less amount of other three compounds), which account 93.73% of the total oil, were identified by gas chromatography and gas chromatography-mass spectrometry. Oxygenated monoterpenes (57.32%) constituted the main fraction, with b-thujone as the main compound (45.04%), followed by the sesquiterpene hydrocarbon chamazulene (22.71%). Undiluted EO showed a large inhibition spectrum against strains of Listeria monocytogenes (34 out of 44), whilst it was ineffective against enterobacteria and salmonellas. The minimum inhibition concentration (MIC) was evaluated for the two most sensitive strains (L. monocytogenes 186 and 7BO) at two cellular concentrations (10 6 and 10 7 CFU ml-1). The lowest MIC (0.625 ll ml-1 , dilution of oil with acetone) was found for strain L. monocytogenes 186 at 10 6 CFU ml-1 .