Nonlinear optical microscopy: use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies (original) (raw)

Nonlinear optical microscopy techniques, specifically second harmonic generation (SHG) and two-photon microscopy (TPEF), have been explored for the automated and quantitative assessment of liver fibrosis. This study emphasizes the limitations of traditional qualitative scoring methods, which suffer from high interobserver variability, by proposing a novel imaging system that delivers objective and reproducible quantification of collagen deposition in liver tissues. The findings indicate that SHG effectively detects collagen fibers' structure without causing damage to the specimen, offering a reliable alternative for diagnosing and staging chronic liver diseases.