“Comparative Assessment Of Glucose Determination In Blood Using Fabricated Colorimeter And Strip-Based Commercial Glucometer” (original) (raw)
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The amount of glucose in human blood should be controlled. Excess or lack of human blood glucose can cause various diseases such as diabetes, heart disease, cholesterol and blindness. To monitor the amount of blood sugar, the blood sugar should be measured periodically. We report the design and development of a simple spectrometer as a non-invasive method to determine the amount of glucose in urine. System calibration was done by mixing the sugar solution and benedict solution which at various concentrations. The measurements were carried out using several colors of light source. Measurement results show that the blue light source gave the best results compared to other LED colors with an average relative error of measurement about 5.8%. The photodetector output voltage decreases exponentially with increasing concentration of sugar solution which is in agreement with the Lambert-Beer law.
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Analytical methods comparison for the determination of blood glucose are essential in clinical laboratory practice as it improves the quality of health care through accurate and reliable clinical decision making. This study was done to assess the analytical performance between the Glucose meters and spectrophotometric methods for blood glucosedetermination.The Glucometer method determined glucose by using the Finetest Auto-coding TM Premium(infopia Co., Korea) and was compared with the spectrophotometer( Kenza 240,biolabo france) using paired data of blood samples analysed respectively from208 patients in the hospital.Data analysis was performed using Analyse-it ® Version 4.6 method validation software. The results shows that the mean value of blood glucose concentrationswere higher in by the spectrophotometric method KENZA 240 (5.76 mmol/l) than by the FINE TEST glucose meter (5.27 mmol/l).Result of t-test analysis revealed a statistically significant difference (p<0.05) between...
Clinical Chemistry and Laboratory Medicine, 1993
We describe a laboratory assessment of five blood glucose meters. The instruments' analytical characteristics under optimum laboratory conditions and examination of potential sources of errors were intercompared. All glucometers produced precise results, and in all but one meter the CV values varied between 1.5% and 6%. CV's for reproducibility and within-day precision of Glucometer Gx were 10.5% and 7.3%. Sample volume, blood incubation time and colour stability of the strip may influence the results of blood glucose measurements with glucose meters. Underloading the sample strip had statistically significant effects on normal blood glucose values for all meters. One Touch II gave also significantly different results when the strip was overloaded. Incubation times shorter or longer than those recommended by the manufacturer influenced the results of all glucose meters. After colour development of the strip, changes were small for Haemo-Glukotest 20-800R strips and Glucotrix, whereas Accutrend glucose strips had to be read immediately following the prescribed incubation time. Comparison of the glucose meter results with those obtained by the hexokinase method showed good correlation coefficients for Reflolux S (r = 0.992), Accutrend (r = 0.988), One Touch II (r = 0.942), Glucometer Gx (r = 0.986) and Glucocard (r = 0.976). Error grid analysis showed that the results of all meters were clinically correct. Variations in haematocrit are known to be a source of errors when blood glucose is determined with a test strip. In the normoglycaemic range the results obtained with Accutrend and Glucocard were not influenced by even extreme haematocrit values. In the high glucose concentration range there was a decrease in blood glucose values with increasing haematocrit for all meters. This error was smaller with Accutrend and Glucocard than with the other meters.
Journal of …, 1987
We evaluated three reflectance meters (Accu-Chek 11, Glucometer 11, and Glucoscan 2000) and two reagent strips (Chemstrip bG and Glucostix) for accuracy and precision in determining blood glucose concentrations in the dog. To evaluate accuracy, we compared results of blood glucose determinations performed on 95 samples using the various strips and meters vs. the glucose concentrations obtained using the glucose-oxidase method on a Beckman Glucose Analyzer. Accuracy was evaluated statistically using least squares regression analysis. To evaluate precision, samples in various ranges of blood glucose concentration were tested repeatedly (20 times within a 1-hour period) on the same reflectance meter. Coefficient of variation (CV) was determined to evaluate reproducibility of results. Overall, there were significant correlations (P < 0.001) between the laboratory glucose values and the blood glucose concentrations obtained with Cbemstrip bG (r = 0.976), Glucostix (r = 0.904), Accu-Chek I1 (r = 0.986), Glucometer I1 (r = 0.91 1) and Glucoscan 2000 (r = 0.944). In the precision study, all three meters had excellent CVs in the normal range (3.6% to 4.9%). However, Accu-Chek I1 was found to be more precise in the hypoglycemic and hyperglycemic ranges (3.6% and 2.676, respectively) than either Glucometer I1 (8.8% and 5.4%) or Glucoscan 2000 (7.8% and 8.2%). The results of this study indicate that all of the meters and reagent strips tested are highly accurate in determining blood glucose concentrations in the dog. However, both in terms of accuracy and reproducibility of results, Accu-Chek I1 and Chemstrip bG, gave the highest correlation coefficients and, as such, are probably of the greatest clinical value.
Non-invasive glucose measurement technologies: an update from 1999 to the dawn of the new millennium
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There are three main issues in non-invasive (NI) glucose measurements: namely, specificity, compartmentalization of glucose values, and calibration. There has been progress in the use of near-infrared and mid-infrared spectroscopy. Recently new glucose measurement methods have been developed, exploiting the effect of glucose on erythrocyte scattering, new photoacoustic phenomenon, optical coherence tomography, thermo-optical studies on human skin, Raman spectroscopy studies, fluorescence measurements, and use of photonic crystals. In addition to optical methods, in vivo electrical impedance results have been reported. Some of these methods measure intrinsic properties of glucose; others deal with its effect on tissue or blood properties. Recent studies on skin from individuals with diabetes and its response to stimuli, skin thermo-optical response, peripheral blood flow, and red blood cell rheology in diabetes shed new light on physical and physiological changes resulting from the disease that can affect NI glucose measurements. There have been advances in understanding compartmentalization of glucose values by targeting certain regions of human tissue. Calibration of NI measurements and devices is still an open question. More studies are needed to understand the specific glucose signals and signals that are due to the effect of glucose on blood and tissue properties. These studies should be performed under normal physiological conditions and in the presence of other co-morbidities.
Blood Glucose Measurement with Dextrostix and New Reflectance Meter
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A new instrument, Eyetone, has been produced for use with a Dextrostix reagent strip for estimating blood sugar levels. It differs from the Dextrostix Reflectance Meter in having only one meter scale, a two-point calibration, and a range of measurement limited to 10-400 mg/100 ml. Results with the manufacturers' original calibration were unsatisfactory, but when recalibrated the performance of the instrument gave a regression equation line close to the ideal. The three Eyetone instruments tested were comparable in their accuracy and functional stability. Packed cell volumes in the blood samples in the range of 30-50% had a negligible effect on the results. The Dextrostix-Eyetone method represents a quick and reliable alternative to conventional laboratory methods for blood sugar estimation. It is especially useful for patients outside hospital.
JOURNAL OF ADVANCES IN BIOTECHNOLOGY
In previous studies, the accuracy of glucose measurements were found with significant variations in different self-monitoring devices. This study suggests Hemoglobin a1c (Hba1c) to be used as as an indicator for the accuracy of blood-glucose monitoring devices. In this study, the association between the readings of glycohematoglobin HbA1C and the hyperglycemic readings of thirty hyperglycemic patients is used as an indicator of the accuracy of three types of glucometer devices. The association between hyperglycemic readings and the percentage of HbA1C for the same patients was investigated. The results showed significant association between levels of blood glucose and the percentage of HbA1C in three devices with statistically significant ( p < 0.05). Such relationship is suggested to be used as a relative accuracy of various types of blood glucose self-monitoring devices.
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