Healing of normal and osteopenic bone with titanium implant and low-level laser therapy (GaAlAs): a histomorphometric study in rats (original) (raw)
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Histologic study of the effect of laser therapy on bone repair
The journal of contemporary dental practice, 2008
This study used histologic analysis and HE staining to evaluate laser biomodulation of bone repair in cavities made in the femurs of rats that underwent non-ablative laser irradiation. Eighteen male Wistar rats weighing 300 to 400 grams were randomly assigned to three groups of six animals each. A surgical defect site was produced with a trephine about 2 mm in diameter under abundant irrigation. In Group I the complete surgical protocol to produce a bone defect was followed but without laser radiation (control). In Group II a continuous wave 830 nm infrared laser was used at 10 J/cm2 and 50 mW at each point of the surgical site. In Group III a continuous wave 685 nm infrared laser at 10J/cm2 and 35 mW was used at each point of surgical site. The animals were irradiated at intervals of 48 hours beginning immediately after the preparation of the defect and were sacrificed on the 15th, 21st, and 30th days. Slides were studied by means of descriptive analysis. Greater degrees of new bon...
Low-level laser therapy stimulates bone-implant interaction: an experimental study in rabbits
Clinical Oral Implants Research, 2004
The aim of the present study was to investigate the effect of low-level laser therapy (LLLT) with a gallium-aluminium-arsenide (GaAlAs) diode laser device on titanium implant healing and attachment in bone. This study was performed as an animal trial of 8 weeks duration with a blinded, placebo-controlled design. Two coin-shaped titanium
Laser therapy in bone repair in rats: analysis of bone optical density
Acta ortopedica brasileira, 2014
To investigate, by digital radiology, the bone regeneration process in rats submitted to femoral osteotomy and treated with low power laser therapy. Forty-five Wistar rats were subjected to transverse osteotomy of the right femur and divided randomly into three experimental groups (n = 15): animals not treated with laser therapy G (C), animals that received laser therapy with λ: 660nm G (660nm) and animals that received laser therapy with λ: 830nm G (830nm). Animals were sacrificed after 7, 14 and 21 days. The bone calluses were evaluated by digital X-ray at 65 kVp, 7mA and 0.032 s exposures. The values obtained were submitted to variance analysis (ANOVA) followed by the Tukey-Kramer test. The significance level adopted was 5%. The groups G (C), G (660nm), and G (830nm) at the 7(th) day showed a significant bone development, with p <0.0116; the groups G (C), G (660nm), and G (830nm) at the 14(th) day showed values of p <0.0001; at the 21(st) day,a higher degree of bone repair ...
Lasers in Medical Science, 2011
The present study aimed to evaluate the effects of LLLT (660-and 808-nm wavelengths) on the process of repairing bone defects induced in the femurs of female rats submitted to ovariectomy. Bilateral ovariectomies were performed on 18 female Wistar rats, which were divided into control and irradiated groups after the digital analysis of bone density showed decreased bone mass and after standardized drilling of the femurs. The irradiated groups received 133 J/cm 2 of AsGaAl (660-nm) and InGaAlP (880-nm) laser radiation. The animals were euthanized on days 14 and 21 after the bone defects were established.
Brazilian Dental Journal, 2013
Use of biomaterials and light on bone grafts has been widely reported. This work assessed the influence of low-level laser therapy (LLLT) on bone volume (BV) and bone implant contact (BIC) interface around implants inserted in blocks of bovine or autologous bone grafts (autografts), irradiated or not, in rabbit femurs. Twenty-four adult rabbits were divided in 8 groups: AG: autograft; XG: xenograft; AG/L: autograft + laser; XG/L: xenograft + laser; AG/I: autograft + titanium (Ti) implant; XG/I: xenograft + Ti implant; AG/I/L: autograft + Ti implant + laser; and XG/I/L: xenograft + Ti implant + laser. The animals received the Ti implant after incorporation of the grafts. The laser parameters in the groups AG/L and XG/L were λ=780 nm, 70 mW, CW, 21.5 J/cm 2 , while in the groups AG/I/L and XG/I/L the following parameters were used: λ=780 nm, 70 mW, 0.5 cm 2 (spot), 4 J/cm 2 per point (4), 16 J/cm 2 per session, 48 h interval × 12 sessions, CW, contact mode. LLLT was repeated every oth...
Low-level laser therapy on bone repair: is there any effect outside the irradiated field?
Lasers in Medical Science, 2015
The biological effects of local therapy with laser on bone repair have been well demonstrated; however, this possible effect on bone repair outside the irradiated field has not been evaluated. The aim of this study was to investigate the effect of low-level laser therapy (LLLT) (λ=830 nm) on repair of surgical bone defects outside the irradiated field, in rats. Sixty Wistar rats were submitted to osteotomy on the left femur and randomly separated into four groups (n=15): group I, control, bone defect only; group II, laser applied on the right femur (distant dose); group III, laser applied locally on the bone defect and also on the right femur (local and distant doses); and group IV, laser applied locally on the left femur (local dose). Laser groups received applications within a 48-h interval in one point per session of density energy (DE)= 210 J/cm 2 , P =50 mW, t = 120 s, and beam diameter of 0.028 cm. Five animals of each group were euthanized 7, 15, and 21 days after surgery. Histologic analysis in all groups showed new bone formation in the region of interest (ROI) at 7 days. After 15 days, bone remodeling with a decrease of bone neoformation in the marrow area was observed in all groups. After 21 days, advanced bone remodeling with new bone mostly located in the cortical area was observed. The histomorphometric analysis showed at 7 days a significant increase of bone formation in groups III and IV compared to groups I and II. At days 15 and 21, histomorphometric analysis showed no significant differences between them. Laser therapy presented a positive local biostimulative effect in the early stage of bone healing, but the LLLT effect was not observed a long distance from the evaluated area.
Analysing the Effect of Low Level Laser Therapy on Bone Histomorphometry in Rats
Recent Research Advances in Biology Vol. 11, 2021
The aim of this study was to assess the histological effect of Low Level Laser Therapy (LLLT) (904 nm) on the repair of standardized bone defects on the femur of rats. Sixty male wistar rats were assigned into two equal groups. Group (A: laser group) and group (B: control group). A surgical fracture was done in middle third of femur of all rats. In group (A) a continuous wave 904 nm infrared laser was applied at dose 4 j/cm 2 at fracture site immediately post operative for 7 sessions, each session was 5 minutes. The animals were killed by over dose of general anesthesia on the 15th,30th and 45th days after surgery, The specimens were processed and stained with Hematoxylin-eosin (H/E), special stain Masion trichrome and analyzed by light microscopy. The descriptive analysis of histological imaging showed greater degree of new bone formation, osteoblastic surface and collagen fiber in the irradiated group when compared with the control group. Based on the obtained results, this study concluded that LLLT was efficient in promoting bone healing, and increasing new bone formation in the process of surgically fractured femur in animal study.
Lasers in Medical Science, 2003
Low-level laser therapy (LLLT) is increasingly being used in the regeneration of soft tissue. In the regeneration of hard tissue, it has already been shown that the biomodulation effect of lasers repairs bones more quickly. We studied the activity in bone cells after LLLT close to the site of the bone injury. The femurs of 48 rats were perforated (24 in the irradiated group and 24 in the control group) and the irradiated group was treated with a GaAlAs laser of 660 nm, 10J/cm 2 of radiant exposure on the 2nd, 4th, 6th and 8th days after surgery (DAS). We carried out histomorphometry analysis of the bone. We found that activity was higher in the irradiated group than in the control group: (a) bone volume at 5 DAS (p ¼ 0.035); (b) osteoblast surface at 15 DAS (p ¼ 0.0002); (c) mineral apposition rate at 15 and 25 DAS (p ¼ 0.0008 and 0.006); (d) osteoclast surface at 5 DAS and 25 DAS (p ¼ 0.049 and p ¼ 0.0028); and (e) eroded surface (p ¼ 0.0032). We concluded that LLLT increases the activity in bone cells (resorption and formation) around the site of the repair without changing the bone structure.
Effects of low-level laser therapy on human osteoblastic cells grown on titanium
Braz Dent J, 2010
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix ...
Effect of irradiation of 810nm laser on bone for 10 sec: A rabbit histological study
IOSR Journals , 2019
Introduction and Objective In last decade, low level laser therapy has been evaluated for stimulation and acceleration of bone formation. In spite of promising results, biphasic ‘dose’ response remains. Moreover, the use of single session of low level laser on healing of bone is not explored thoroughly. The aim of this study was to determine the optimal ‘dosage’ for formation of bone using diode laser of 810nm under single irradiation. Materials and Methods Six New Zealand male rabbits were used weighing 1.5-2 Kgs and 8-10 months old for the study. Femur was chosen as site of surgery. The centre of the femur was drilled using implant osteotomy drills to the size of 2.8mm in width and 6mm in depth. 810nm Diode laser (GaAlAs, AMD Picasso®) was used in this study. Laser parameters were, wavelength of 810nm, power of 90mW, time of 10 seconds in continuous mode using the disposable fibre of 300µmm diameter in light noncontact. Contra lateral femur was used as a control and the laser was sham treated. At the end of 2 weeks samples were collected from the surgical area and slides were prepared and analysed histologically. Results At 14th day, the lased group showed slight areas of haemorrhage in marrow cavity with no evidence of bone formation in lased site. Conclusion The results of the present study using 810nm, 90mW, for 10 sec for single session did not stimulate formation of new bone in two weeks.