Healing of normal and osteopenic bone with titanium implant and low-level laser therapy (GaAlAs): a histomorphometric study in rats (original) (raw)

Abstract

The study investigates the influence of low-level laser therapy (LLLT) on bone healing in the femur of osteopenic and normal rats with titanium implants. Ovariectomy and control group were randomly submitted to LLLT, which was applied by gallium-aluminum-arsenium (GaAlAs) laser at the surgical site before and after placing the implant, for seven times. Histomorphometric and statistical analysis were performed. Most irradiated groups showed higher values than the nonirradiated groups. The GaAlAs infrared diode laser may improve the osseointegration process in osteopenic and normal bone, particularly based on its effects in the initial phase of bone formation.

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References (39)

  1. Pinheiro ALB, Gerbi MEMM (2006) Photobioengineering of the bone repair process. Photomed Laser Surg 24:169-178
  2. Deco C, Marchini A, Barbara MA, Vasconcellos L, Rocha R, Marchini L (2011) Negative effects of alcohol intake and estrogen deficiency combination on osseointegration in a rat model. J Oral Implantol 37:633-639
  3. Stübinger S, Ghanaati S, Saldamli B, Kirkpatrick CJ, Sader R (2009) Er: YAG laser osteotomy: preliminary clinical and histological results of a new technique for contact-free bone surgery. Eur Surg Res 42:150-156
  4. Genant HK, Cooper C, Poor G et al (1999) Interim report and recommendations of the World Health Organization Task Force for osteoporosis. Osteoporos Int 10:259-264
  5. Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:171-192
  6. Frost HM, Jee WS (1992) On the rat model of human osteopenics and osteoporoses. Bone Miner 18:227-236
  7. Pires-Oliveira DAA, Oliveira RF, Amadei SU, Pacheco-Soares C, Rocha RF (2010) Laser 904 nm action on bone repair in rats with osteoporosis. Osteoporos Int 21:2109-2114
  8. Renno ACM, Moura F, Bossini P, Parizotto N (2006) Effects of 830 nm laser used in two doses, on biomechanical properties of osteopenic rats. Photomed Laser Surg 24:204-208
  9. Gerbi MEMM, Pinheiro ALB, Marzola C et al (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23:382-388
  10. Gerbi MEMM, Marques AMC, Ramalho LMP et al (2008) Infra- red laser light further improves bone healing when associated with bone morphogenic proteins: an in vivo study in a rodent model. Photomed Laser Surg 26:55-60
  11. Lopes CB, Pinheiro ALB, Sathaiah S, Silva NS, Salgado MAC (2007) Infrared laser photobiomodulation (830 nm) on bone tissue around dental implants: a raman spectroscopy and scanning electronic microscopy study in rabbits. Photomed Laser Surg 25:96-101
  12. Diniz JS, Nicolau RA, de Melo Ocarino N, do Carmo Magalhães F, de Oliveira Pereira RD, Serakides R (2009) Effect of low-power gallium-aluminum-arsenium laser therapy (830 nm) in combina- tion with bisphosphonate treatment on osteopenic bone structure: an experimental animal study. Lasers Med Sci 24:347-352
  13. Obradovic RR, Kesic LG, Pesevska S (2009) Influence of low- level laser therapy on biomaterial osseointegration: a mini review. Lasers Med Sci 24:447-451
  14. Favaro-Pipi E, Feitosa SM, Ribeiro DA, Bossini P, Oliveira P, Parizotto NA, Renno AC (2010) Comparative study of the effects of low-level therapy on bone defects in tibias of rats. Lasers Med Sci 25:727-732
  15. Bossini PS, Rennó AC, Ribeiro DA et al (2012) Low level laser therapy (830 nm) improves bone repair in osteoporotic rats: similar outcomes at two different dosages. Exp Gerontol 47:136-142
  16. Nogueira GT, Mesquita-Ferrari RA, Souza NH et al (2012) Effect of low-level laser therapy on proliferation, differentiation, and adhesion of steroid-treated osteoblasts. Lasers Med Sci 27:1189-1193
  17. Nissan J, Assif D, Gross MD, Yaffe A, Binderman I (2006) Effect of low intensity laser irradiation on surgically created bony defects in rats. J Oral Rehabil 33:619-624
  18. Doshi-Mehta G, Bhad-Patil WA (2012) Efficacy of low-intensity laser therapy in reducing treatment time and orthodontic pain: a clinical investigation. Am J Orthod Dentofac Orthop 141:289-297
  19. Pretel H, Lizarelli RFZ, Ramalho LTO (2007) Effect of low-level laser therapy on bone repair: histological study in rats. Lasers Surg Med 39:788-796
  20. Pinheiro ALB, Limeira FA Jr, Gerbi MEMM, Ramalho LMP, Marzola C, Ponzi EAC (2003) Effect of low-level therapy on the repair of bone defects grafted with inorganic bovine bone. Braz Dent J 14:177-181
  21. Ré Poppi R, Da Silva AL, Nacer RS et al (2011) Evaluation of the osteogenic effect of low-level laser therapy (808 nm and 660 nm) on bone defects induced in the femurs of female rats submitted to ovariectomy. Lasers Med Sci 26:515-522
  22. Pinheiro ALB, Oliveira MG, Martins PPM, Ramalho LMP, Oliveira MAM, Junior NA, Nicolau RA (2001) Biomodulatory effects of low-level laser therapy on bone regeneration. Laser Ther 13:73-79
  23. Dörtbudak O, Haas R, Mailath-Pokorny G (2002) Effect of low- power laser irradiation on bony implant sites. Clin Oral Implants Res 13:288-292
  24. Merli LAS, Santos MT, Genovese WJ, Faloppa F (2005) Effects of low-intensity laser irradiation on process of bone repair. Photomed Laser Surg 2:212-215
  25. Duarte PM, César Neto JB, Gonçalves PF, Sallum EA, Nociti FH (2003) Estrogen deficiency affects bone healing around titanium implants: a histometric study in rats. Implant Dent 12:340-346
  26. Prado RF, Silveira VAS, Rocha RF, Vasconcellos LMR, Carvalho YR (2012) Effects of experimental osteoporosis and low calcium intake on post-extraction sockets of rats. Int J Exp Pathol 93:139- 147
  27. Khadra M (2005) The effect of low level laser irradiation on implant-tissue interaction. In vivo and in vitro studies. Swed Dent J 172:1-63
  28. Omasa S, Motoyoshi M, Arai Y, Ejima K, Shimizu N (2012) Low-level laser therapy enhances the stability of orthodontic mini-implants via bone formation related to bmp-2 expression in a rat model. Photomed Laser Surg 30:255-261
  29. Naka T, Yokose S (2012) Application of laser-induced bone therapy by carbon dioxide laser irradiation in implant therapy. Int J Dent. doi:10.1155/2012/409496
  30. Renno ACM, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on the osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25:275-280
  31. Ng GY, Fung DT, Leung MC, Guo X (2004) Ultrastructural comparison of medial collateral ligament repair after single or multiple applications of GaAlAs laser in rats. Lasers Surg Med 35:317-323
  32. Reis FA, Belchior AC, de Carvalho PT et al (2009) Effect of laser therapy (660 nm) on recovery of the sciatic nerve in rats after injury through neurotmesis followed by epineural anastomosis. Lasers Med Sci 24:741-747
  33. Garavello-Freitas I, Baranauskas V, Joazeiro P, Padovani CR, Dal Pai-Silva M, Cruz-Hofling MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochemical Photobiol 70:81-89
  34. Sasaki Y, Wang S, Ogata Y (2011) Transcriptional regulation of bone sialoprotein gene by CO (2) laser irradiation. J Oral Sci 53:51-59
  35. Grassi FR, Ciccolella F, D'Apolito G et al (2011) Effect of low- level laser irradiation on osteoblast proliferation and bone formation. J Biol Regul Homeost Agents 25:603-601
  36. Serakides R, Nunes VA, Ocarino NM, Nascimento EF (2004) Bone effects of the association hyperthyroidism-castration of adult female rats. Arq Bras Endocrinol Metabol 48:875-883
  37. Saad A, El Yamany M, Abbas O, Yehia M (2010) Possible role of low level laser therapy on bone turnover in ovariectomized rats. Endocr Regul 44:155-163
  38. Garavello I, Baranauskas V, Cruz-Hofling MA (2004) The effects of low laser irradiation on angiogenesis in injured rat tibiae. Histol Histopathol 19:43-48
  39. Lill CA, Hesseln J, Schlegel U, Eckhardt C, Goldhahn J, Schneider E (2003) Biomechanical evaluation of healing in a non-critical defect in a large animal model of osteoporosis. J Orthop Res 21:836-842