Tetranucleotide microsatellite markers in Ctenomys torquatus (Rodentia) (original) (raw)

2011, Conservation Genetics Resources

Eleven microsatellite markers were isolated from an enriched library developed specifically for the tuco-tuco Ctenomys torquatus, using tri and tetranucleotide probes. Ten of these were successfully amplified, and only one was monomorphic for the populations that have so far been analyzed. Analysis of two different populations yielded a mean of 2.6 (Cachoeira do Sul-CAC) and 4.3 (Butiá-BUT) alleles per locus, with a range from 1 to 6. The means for observed and expected heterozygosity were also lower in the CAC population (Ho = 0.37 and He = 0.39), but in BUT they were 0.63 and 0.61 respectively. These are the first microsatellite markers reported for a Brazilian species of tuco-tuco, and will be applied to investigate the genetic structure of their populations in the widely impacted Pampa Biome.