Glutathione peroxidase, superoxide dismutase and catalase activities in children with chronic hepatitis (original) (raw)
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Oxidative Stress in Children Chronic Hepatitis
Romanian Journal of Pediatrics, 2017
Oxidative stress reduces the efficacy of the immune response effector mechanisms, making the cells more susceptible to apoptosis. In lymphoid cells, free radicals interfere with the response to antiviral treatment, causing resistance to therapy and favoring chronic infection. One of the factors responsible for oxidative stress in chronic infection with hepatitis B is the production of pro-inflammatory cytokines. On the other hand, studies show that C hepatitis virus can directly induce oxidative stress in hepatocytes. Chronic C hepatitis core gene expression was associated with elevated reactive oxygen species markers, decreased intracellular and/or mitochondrial glutation content and increased level of oxidized thioredoxin and lipid peroxidation products. Moreover, increased level of the malonyl-dialdehyde in serum of patients with hepatocarcinoma is suggesting that MDA can be a reliable marker of liver injury quantification. Reduction of the oxidative stress can be done using antioxidant medication (hepatoprotectives, ursodeoxycholic acid and vitamin A, C, E), this approach representing an important benefit for the patient.
Oxidative Stress In Patients With Chronic Hepatitis B And C
Medical Journal of Trakya University, 2010
Objective: Hepatitis B and hepatitis C virus infections are extensively seen throughout the world. Disturbances in the antioxidant system and oxidative stress may play a role in the pathogenesis of chronic liver diseases. The aim of this study was to evaluate the oxidant/antioxidant status in the serum of patients with chronic hepatitis B (CHB) and C (CHC). Material and Methods: Twenty patients with CHB, 20 with CHC and 30 healthy volunteers were included in this study. Catalase (CAT), superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels were measured in all patients and control group specthrophotometrically. Results: While CAT and SOD activities were significantly lower in patients with CHC, these enzymes were significantly higher in patients with CHB compared to controls. However, MDA levels were increased in each patient group as compared to the control group. Also, antioxidant enzyme activities were found to be significantly lower and MDA levels were higher in patients with CHC, compared to patients with CHB. Conclusion: We considered that insufficiency of antioxidant barrier may cause oxidative stress in patients with CHB and CHC, so antioxidant treatment should be useful for these patients.
European Journal of Inflammation, 2004
Damage of hepatitis B virus (HBV) and hepatitis C virus (HCV)-infected hepatocytes is mediated by both a direct cytopathic effect of the viruses and by immunologic response of T-cells, However, other mechanisms such as oxidative stress, may also be involved in cellular damage. We conducted a study to investigate the status of superoxide dismutase activity (SOD) and the total antioxidant status (TAS) in a consecutive cohort of patients infected with HBV and HCV infections, including HBV patients in the chronic inactive state of the disease. The latter were included in an attempt to determine whether antioxidant status is affected even in cases where an obvious viral activity is absent. T AS and SOD were measured in 55 patients (43 HBV, 12 HCV) and 25 healthy controls. 17 out of 43 patients had chronic inactive HBV state, 15 had chronic hepatitis Band 11 had HBV-related cirrhosis. In the HCV group, 6 patients had chronic hepatitis C and 6 HCV-related cirrhosis. Erythrocyte SOD activity was determined in haemolysate from red blood cells using a kinetic spectrophotometer method. T AS was measured by a colorimetric assay. The mean TAS and SOD values in the total number of patients (1.20±O.12 mmol/L and 1040±255 Ulg Hb, respectively) were significantly lower (p<O.OOI) compared to healthy controls (1.57±O.13 mmol/L and 1491±420 D/g Hb), Comparisons between groups showed significantly lower (p<O.OOI) TAS and SOD values in each subgroup of patients compared to healthy controls. A significant positive correlation was found between TAS and SOD in the total number of patients (n= 55, p<O.OI), in the chronic hepatitis group (n=21, p=O.OI) and in the total group of HBV patients (n=43, p<O.OI). We demonstrated a significant reduced antioxidant capacity in patients with chronic HBV and HCV as indicated by low TAS and SOD. These findings were independent of the virological, biochemical and clinical status of the patients, including those with chronic inactive HBV state. This could suggest that the tissue-related consequences of oxidative stress might start from the inactive stage of liver viral diseases. However, our observations should be viewed with caution and need to be tested in a larger numbers of patients in order to determine prospectively whether these findings have pathophysiological and/or clinical significance.
Journal of Medical Science And clinical Research, 2016
Background: The exact pro-oxidant and antioxidant status in alcoholic hepatitis is still not clear. Material & Methods: The present study was conducted in Department of Biochemistry, RMRI, Bareilly and Santosh medical college & Hospital. 35 alcoholic hepatitis patients were subjected to detailed clinical examination and laboratory investigations and the results were compared with 35 controls. Blood samples were collected for oxidative stress parameters. It was observed that there was a significant increase in activities of SOD, GPX, MDA and Catalase activity in patients with alcoholic hepatitis when compared to controls. Results: Results of our study depict higher oxygen free radical production, evidenced by elevated levels of MDA and decreased levels of GSH, ascorbic acid, vitamin-E and Catalase activity, supporting the evidence of oxidative stress in alcoholic hepatitis patients. Increased activities of antioxidant enzymes might be a compensatory regulation of body in response to increased oxidative stress. Decreased concentrations of antioxidant vitamins support the hypothesis that alcoholic hepatitis is an important causative factor in pathogenesis of lipid peroxidation. Conclusion: These data reveal that antioxidant defense mechanisms might be impaired in patients with alcoholic hepatitis. These findings also provide a theoretical basis for development of novel therapeutic strategies, such as antioxidant supplementation.
Liver International, 2006
Aims: Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress-related molecules remains to be clarified. Methods: 4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), catalase, superoxide dismutase-1 (SOD-1), glutathione peroxidase-1, thioredoxin (TRX) in leukocytes and urinary 8-hydroxy-2 0 -deoxyguanosine (8-OHdG) were examined in 164 persons, including 130 chronic viral hepatitis patients and 34 normal individuals, by Western blot analysis and enzyme-linked immunosorbent assay, respectively. Hepatic expression of these proteins was immunohistochemically examined in 12 patients with chronic viral hepatitis, compared with three persons without liver damage. Results: The 4-HNE/b-actin ratios in chronic viral hepatitis were significantly higher than those in normal individuals (Po0.01), and were significantly correlated with asparate aminotransferase (AST) and alanine aminotransferase (ALT) (Po0.01, each). The catalase/b-actin and SOD-1/ b-actin ratios in chronic viral hepatitis were higher than those in normal individuals, and were significantly correlated with 4-HNE/b-actin ratios (Po0.01, each). Hepatic expression of 4-HNE, 4-HHE, catalase, SOD-1 and TRX in chronic viral hepatitis was higher than that without liver damage. Urinary excretion of 8-OHdG was not changed in chronic viral hepatitis.
Superoxide dismutase activity in children with chronic liver diseases
Journal of Hepatology, 2000
Background/Aims: Liver disease in infancy has multiple etiologies. As reactive oxygen interm~at~ are involved in several types of tissue damage, we have investigated whether different forms of liver disease in infancy are associated with increased free radical generation, using an indirect approach in which superoxide dismutase (a free radical scavenger) activity is determined in the liver tissue. methods: A total of 48 liver biopsies performed at diagnosis were evaluated retrospectively. Nine infants had biiary atresia, eight Alagille syndrome, seven alantitrypsin deficiency and 12 cryptogenic hepatitis. As controls we studied 12 biopsies with normal histology obtained from seven children with portal vein thrombosis and five children who underwent biopsy for management reason but had no liver disease. Superoxide dismutase activity in liver biopsy specimens was measured using the cytocbrome C method by spectrophotometry and expressed as U SODlmg protein. Rest&s: Superoxide dismuta~ activity was significantly increased in biliary atresia (1.25-C0.56 U IVER DISEASE in infancy has multiple etiologies, re-L sulting in a heterogeneous set of insults: infection, metabolic abnormality and anatomic anomalies (l-5). There is a considerable overlap between disorders involving the hepatocyte or the biliary apparatus in both clinical features and initial and subsequent sites of injury (6). Whether different mechanisms of tissue damage are involved in the different diagnostic categories remains to be clarified. In recent years, there has been increasing interest in the possible involvement of reactive oxygen intermediates (ROI) as pathogens in general and in the pathophysiology of liver injury in par
Glutathione peroxidase and malondialdehyde in children with chronic hepatitis C
Clinical & Experimental Hepatology, 2019
Aim of the study: We aimed to assess oxidative stress factors, glutathione peroxidase (GPX) and malondialdehyde (MDA) in children with chronic hepatitis C (CHC) and their relation to treatment response. Material and methods: The study included 50 children with chronic hepatitis C virus (HCV) before treatment (naïve HCV), 25 children responders to HCV treatment, 25 children non-responders to HCV treatment and 25 healthy controls. All patients and controls were subjected to GPX and MDA measurement by enzyme-linked immunosorbent assay. Results: The average GPX activity in erythrocytes of naïve CHC patients was 29.2 ±10.3 mU/ml. It was statistically significantly lower than the average activity of GPX in erythrocytes of the healthy control group (47.3 ±5.2 mU/ml) (p < 0.05). The average GPX activity in erythrocytes of the responder group was 34.93 ±3.17 mU/ml. It was statistically significantly higher than the average activity of GPX in erythrocytes of the non-responder group (11.7 ±4.2 mU/ml) (p < 0.05). Plasma MDA was significantly higher in naïve CHC patients than in healthy controls (9.7 ±3.7 nmol/ml vs. 3 ±1.1 nmol/ml, p < 0.0001). Furthermore, plasma MDA concentration was significantly decreased in the responder group (5.36 ±0.7 nmol/ml) and elevated in the non-responder group (16.05 ±2.9 nmol/ml). Conclusions: Lower pretreatment levels of GPX and higher MDA level might be markers of oxidative stress occurring in HCV patients. Reversal of changes of these levels with completion of the treatment may indicate a correlation between oxidative stress and the viral pathogenesis.
Study of lipid peroxide levels and status of antioxidant enzymes in alcoholic hepatitis patients
Journal of Medical Science And clinical Research, 2018
Background: The real status of pro-oxidant and antioxidant in alcoholic hepatitis is still not clear. Material & Methods: This study was conducted in Department of Biochemistry, Santosh medical college & Hospital. 40 alcoholic hepatitis patients were subjected to detailed clinical examination and laboratory investigations and the results were compared with 40 controls. Blood samples were collected for oxidative stress parameters. It was observed that there was a significant increase in activities of SOD, MDA and Catalase activity in patients with alcoholic hepatitis when compared to controls. Results: Result of this study depict higher oxygen free radical production, evidenced by elevated levels of MDA and decreased levels of Catalase activity, supporting the evidence of oxidative stress in alcoholic hepatitis patients. Increased activities of antioxidant enzymes might be a compensatory regulation of body in response to increased oxidative stress. Conclusion: Data of this study represent that antioxidant defense mechanisms might be impaired in patients with alcoholic hepatitis. These findings also provide a theoretical basis for development of novel therapeutic strategies, such as antioxidant supplementation.
Antioxidant status and lipid peroxidation activity in evaluating hepatocellular damage in children
Eastern Mediterranean Health Journal, 2009
Serum levels of glutathione reductase (GR), glutathione S-transferase-α (GST-α) and malondialdehyde (MDA) were determined to evaluate their use in diagnosing hepatocellular damage in 75 children with liver disease. Except for level of GR in patients with HBV, GR, GST-α and MDA were raised significantly in patients compared with controls. At 100% specificity, the sensitivity of the 3 markers for detecting hepatitis B virus, hepatitis C virus and schistosomiasis infection respectively were: 16.7%, 100.0% and 17.7% for GR; 33.3%, 62.1% and 38.2% for GST-α; and 25.0%, 10.3% and 29.4% for MDA. GR was more sensitive in hepatitis C infection, while MDA reflected changes in liver ultrasound and GST-α was the best indicator for histopathological changes.