Techniques: GPCR assembly, pharmacology and screening by flow cytometry (original) (raw)
Ligand-receptor-G-protein molecular assemblies on beads for mechanistic studies and screening by flow cytometry
Eric Prossnitz
Molecular pharmacology, 2003
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Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis
Larry Sklar
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A Rapid and Efficient Immunoenzymatic Assay to Detect Receptor Protein Interactions: G Protein-Coupled Receptors
Simona Daniele
International Journal of Molecular Sciences, 2014
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Chapter 11 Subsecond Analyses of G‐Protein Coupled‐Receptor Ternary Complex Dynamics by Rapid Mix Flow Cytometry
Tione Buranda
Methods in Enzymology, 2009
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A generic microfluidic biosensor of G protein-coupled receptor activation—monitoring cytoplasmic [Ca2+] changes in human HEK293 cells
Margriet Roelse
Biosensors and Bioelectronics, 2013
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Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides
Eric Prossnitz
Analytical Biochemistry, 2007
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Evaluation of Dynamic Mass Redistribution Technology for Pharmacological Studies of Recombinant and Endogenously Expressed G Protein-Coupled Receptors
Alice Gao
Assay and Drug Development Technologies, 2008
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Fluorescent protein complementation assays: new tools to study G protein-coupled receptor oligomerization and GPCR-mediated signaling
Val Watts
Molecular and Cellular Endocrinology, 2011
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Air-Stable G Protein-Coupled Receptor Microarrays and Ligand Binding Characteristics
Ann Ferrie
Analytical Chemistry, 2006
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Functional characterisation of G protein-coupled receptors
David Poyner
Methods (San Diego, Calif.), 2018
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Fluorescence Analysis of Receptor−G Protein Interactions in Cell Membranes †
William Lim
Biochemistry, 2002
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Detecting ligand interactions with G protein-coupled receptors in real-time on living cells
Hanna Björkelund, Dan Larhammar
Biochemical and Biophysical Research Communications, 2013
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Antibodies against G-protein coupled receptors: novel uses in screening and drug development
kuoy pheap
Combinatorial chemistry & …, 2008
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Aequorin functional assay for characterization of G-protein-coupled receptors: Implementation with cryopreserved transiently transfected cells
sandra flores
Analytical Biochemistry, 2010
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Expression and purification of recombinant G protein-coupled receptors: A review
Abigail otchere
Protein Expression and Purification, 2020
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G-protein-coupled receptors in drug discovery: nanosizing using cell-free technologies and molecular biology approaches
Richard Glatz
Journal of …, 2005
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An escort for GPCRs: implications for regulation of receptor density at the cell surface
Stefano Marullo
2008
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Monitoring the formation of dynamic G-protein-coupled receptor–protein complexes in living cells
K. Pfleger
Biochemical Journal, 2005
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Flow cytometry for drug discovery, receptor pharmacology and high-throughput screening
Larry Sklar
Current Opinion in Pharmacology, 2007
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G Protein-Coupled Receptor Multimers: A Question Still Open Despite the Use of Novel Approaches
Jean-philippe Pin
Molecular pharmacology, 2015
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Miniaturization of Whole Live Cell-Based GPCR Assays Using Microdispensing and Detection Systems
todd smith
Journal of Biomolecular Screening, 2004
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Multi-pathway in vitro pharmacological characterisation of specialised pro-resolving G protein-coupled receptors (SPM-GPCRs)
Darren M Riddy
Molecular Pharmacology, 2022
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Validation of a rapid, non-radioactive method to quantify internalisation of G-protein coupled receptors
Astrid Alewijnse
Naunyn-Schmiedeberg's Archives of Pharmacology, 2007
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G protein-coupled receptor quantification using peptide group-specific enrichment combined with internal peptide standard reporter calibration
Oliver Poetz
Journal of Proteomics, 2013
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