Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine (original) (raw)

Journal of Global Pharma Technology, 2017

Abstract

Abstract : Objective: to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell ( UC- MSC) culture waste at harvest. Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests. We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium waste s using ELISA method. We grouped GF concentrations according to various culture length sat harvest , and calculated them ean values and standard deviations of the various GF . We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration . Results: EGF , VEGF and PlGF concentrations were highest at harvest day-3, while NGF concentration was the highest at harvest-day-5 , respectively . Therefore, overall highest concentration of most GF was at harvest day 3. Conclusion: UC- MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF , which might be used in regenerative medicine.

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