Analysis of the Essential Oil of Artemisia Absinthium in Two Harvest Times (original) (raw)
Related papers
2013
The objective of this study was the valorization of the essential oils from Spanish Artemisia absinthium domesticated plants from Teruel and Sierra Nevada (Spain). These populations were experimentally cultivated in the field and under controlled conditions. The insect antifeedant properties of their essential oils collected yearly from two locations were tested against Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi. Additionally we studied their phytotoxic, antifungal and antiparasitic effects. The oils from cultivated A. absinthium were characterized by the presence of cis-epoxyocimene, chrysanthenol, and chrysanthenyl acetate. The variations observed in oil composition were mostly quantitative but also qualitative. (Z)-2,6-Dimethyl-5,7-octadien-2,3-diol has been isolated and identified by NMR. Among the oil samples, these rich in cis-epoxyocimene and sesquiterpenes were the most active ones against S. littoralis. (Z)-2,6-Dimethyl-5,7-octadien-2,3-diol showed moderate activity against S. littoralis. The strongest antifeedant effects were found for commercial A. absinthium oil samples rich in thujones and sabinyl acetate. F. oxysporum and F. solani were affected by oils from cultivated A. absinthium and commercial oil samples. Oils from cultivated A. absinthium showed antiparasitic effects against Leishmania infantum and Trypanosoma cruzi with better results than the commercial samples.
Journal of Agricultural and Food Chemistry, 2006
The chemical composition of the aerial and root essential oils, hydrodistilled from Artemisia absinthium L. and Artemisia vulgaris L. (wild-growing populations from Serbia), were studied by gas chromatography, gas chromatography-mass spectrometry, and 13 C nuclear magnetic resonance. During the storage of plant material under controlled conditions, a significant decrease of essential oil yields (isolated directly after drying and after 1 year of storage) and significant differences in their chemical compositions were observed. A possible mechanism for the observed oil component interconversion has been discussed. The noticeable differences in the chemical composition of the oils isolated from roots and aerial parts of A. absinthium and A. vulgaris were also correlated with the diverging biosynthetic pathways of volatiles in the respective plant organs. The antimicrobial activities against the common human pathogens of all of the isolated oils were tested according to National Committee on Clinical Laboratory Standards. The oils showed a broad spectrum of antimicrobial activity against the tested strains. Therefore, these oils can be used as flavor and fragrance ingredients.
Mass propagation and essential oil analysis of Artemisia vulgaris
Journal of Bioscience and Bioengineering, 2008
Artemisia vulgaris L. (Mugwort) is a threatened and valuable medicinal plant. Attempts have been made in this research to mass propagate its plantlets through in vitro liquid culture technology using Murashige and Skoog (MS) basal medium supplemented with 6-benzyl adenine (BA) (0.44-8.88 microM). Initially, 22.6 shoots (99.9% shooting frequency) developed from shoot tip explants cultured in MS with 4.44 microM BA at 100 ml flask capacity. This was further subcultured at increasing flask capacity (150, 250, and 500 ml) for shoot proliferation. Of the different concentrations of BA and flask capacities tested, 4.44 microM BA and 500 ml flask capacity were found to produce a maximum of 85.5 shoots after 30 d of culture. Shoot proliferation was found to increase with increasing flask capacity whereas shoot number decreased with increasing BA concentration (>4.44 microM). Individual shoots were isolated and rooted on MS medium containing 8.56 microM indole-3-acetic acid (IAA). Then the plantlets were acclimatized under standard laboratory conditions and later under greenhouse conditions. Fresh leaves were collected from greenhouse-grown plants and subjected to essential oil analysis by the simultaneous distillation and extraction method. GC-MS results revealed the presence of 88 components and the extracted oil was rich in camphor (16.8%), alpha-thujone (11.3%), germacrene D (7.2%), camphene (6.5%), 1,8-cineole (5.8%) and beta-caryophyllene (5.4%). This in vitro strategy can be a reliable method for the steady production of a large number of plants for essential oil production, which is reported for the first time for A. vulgaris.
Planta medica, 2014
The objective of this study was the valorization of the essential oils from Spanish Artemisia absinthium domesticated plants from Teruel and Sierra Nevada (Spain). These populations were experimentally cultivated in the field and under controlled conditions. The insect antifeedant properties of their essential oils collected yearly from two locations were tested against Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi. Additionally we studied their phytotoxic, antifungal and antiparasitic effects. The oils from cultivated A. absinthium were characterized by the presence of cis-epoxyocimene, chrysanthenol, and chrysanthenyl acetate. The variations observed in oil composition were mostly quantitative but also qualitative. (Z)-2,6-Dimethyl-5,7-octadien-2,3-diol has been isolated and identified by NMR. Among the oil samples, these rich in cis-epoxyocimene and sesquiterpenes were the most active ones against S. littoralis. (Z)-2,6-Dimethyl-5,7-octadien-2,3-diol showed moderate activity against S. littoralis. The strongest antifeedant effects were found for commercial A. absinthium oil samples rich in thujones and sabinyl acetate. F. oxysporum and F. solani were affected by oils from cultivated A. absinthium and commercial oil samples. Oils from cultivated A. absinthium showed antiparasitic effects against Leishmania infantum and Trypanosoma cruzi with better results than the commercial samples.
Wormwood (Artemisia Absinthium L.): Chemical Composition and Antioxidant Activity of Essential Oil
2020
Wormwood (Artemisia absintium L.) is an aromatic and medicinal plant widespread in the northern hemisphere. Its many biological properties have been identified for wormwood extracts and essential oils. Numerous researchers have investigated the composition of the essential oil and its antioxidant properties. The variability of the chemical composition of the essential oil influences its physiological characteristics, organoleptic properties and its applications. For this reason, several studies have been performed that have analysed the essential oil from different parts of the plant, leaves and flowers, as well as the composition of this oil in different periods of plant vegetation. Temperature and light intensity also influence the chemical composition and antioxidant activity of Artemisia absintium L. essential oil. The antioxidant capacity of the oil is directly proportional to the content of flavonoids and polyphenols, and varies depending on the geographical region from which ...
Growth and Essential Oil Yield of Artemisia (Artemisia annua L.) as Affected by Growth Stage
A study was conducted to observe the growth trend and essential oil yield of Artemisia annua L. under varying growth ages at the Wondo Genet Agricultural Research Center. The experiment consisted of six growth stages (2, 3, 4, 5, 6 and 7 months after transplanting). The treatments were arranged in randomized complete block design with three replications. Data on plant height, branch number/plant, fresh leaf weight/plant, dry leaf weight/plant, fresh leaf/stem ratio, dry leaf/stem ratio essential oil content (EOC) and essential oil yield (EOY) were collected and analyzed. As mean squares from analysis of variance indicated, different growth ages affected plant height, fresh leaf/stem ratio and dry leaf/stem ratio very highly significantly (P < 0.001) and affected EOC and EOY highly significantly (P < 0.01). Fresh leaf weight/plant and dry leaf weight/plant were affected significantly (P < 0.05) by different growth ages. Branch number/plant did not vary significantly (P > 0.05) at different age of growth. In this study, the highest values were recorded at 5 months after transplanting (MAT) for plant height (194 cm), fresh leaf weight/plant (382.47 g) and dry leaf weight/plant (98.36 g) and at 2 MAT for fresh leaf/stem ratio (0.69) and dry leaf/stem ratio (0.64). The highest EOC (1.08%) and EOY (21.78 kg) values were obtained at 6 MAT.
Artemisia absinthium Linn. (Wormwood) is an important perennial shrubby plant that belongs to the family Asteraceae. It is used as herbal medicine in Ayurveda, Homeopathy, Unani and Siddha. It has a great importance as a folk medicine in ancient history from the time of Greek. It is used medically with a very long tradition as anti-helminthic, but it is also used as an ingredient in the liquor absinthe. This species is globally distributed from Europe to North Asia. Flowering occurs in midsummer, pale yellow, tubular flowers develop in drooping heads in the axils of the leaves. A single plant can produce 50,000 seeds. Wormwood invades open and disturbed sites such as pastures, rangelands, crop land, stream banks, prairies .Within India; it has been recorded in the Himalayan region across Jammu & Kashmir. This review provides information on the history, ethno botany, Phytochemistry, medicinal uses, and pharmacological evaluation studies of Artemisia absinthium.
Essential Oil Composition of Artemisia Vulgaris Grown in Egypt
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: The objective of this research was to evaluate the significance of the plant's origin and to assess the essential oil composition of Artemisia vulgaris grown in Egypt simultaneously evaluating the effect of environmental conditions on essential oil composition.
Journal of Applied Pharmaceutical Science, 2016
This study was conceived to investigate the composition of four essential oils (EOs) extracted by hydrodistillation from four parts (leaves, stems, leaves/stems, roots) of Artemisia herba-alba growing wild in the Center of Tunisia. For this, Artemisia herba-alba aerial and roots parts were shade dried with ventilation at room temperature. Then, plant different parts were cut into small pieces and subjected to hydrodistillation using a Clevenger-type apparatus. The gas chromatography (GC) analyses were accomplished with a HP-5890 Series II instrument. The main results showed a total of 152 compounds detected and identified by GC and GC-MS and accounting for 91.3-99.7% of the whole oil. The four oils were characterized by the predominance of monoterpene derivatives (68.2-99.5%) and the major volatile constituent was α-thujone (18.2-45.5%). Qualitative and quantitative differences between the four essential oils have been noted for some compounds. The main compounds of leaves essential oil were α-Thujone (45.5%), β-Thujone (11.4%), trans-sabinyl acetate (10.1%), 1,8-Cineole (7.4%) and camphor (6.8%). α-Thujone (27.5%) was also the main compound in the essential oil of leaves/stems, followed by camphor (22.9%), 1,8-cineole (8.3%), β-thujone (8.2%) and camphene (5.6%). The essential oil of stems was dominated by α-Thujone (28%) followed by β-Thujone (11.4%) and chrysantenone (11%). In the essential oil of roots, α-thujone was less represented (18.2%), followed by camphor (14.6%) and curcumen-15-al (14.3%). It is important to mention that curcumen-15-al has been reported for the first time in Artemisia herba-alba oil Our results revealed avariability in the chemical composition and the yield of the EOs from Artemisia herba-alba. Moreover, curcumen-15-al is a new chemotype first found in Artemisia herba-alba from Tunisia.