Abstract 628: Circulating Exosomes Isolated From Septic Mice Induce Endothelial Hyperpermeability Through Promoting Podosome Cluster Formation (original) (raw)

Arteriosclerosis, Thrombosis, and Vascular Biology, 2017

Abstract

Introduction: Septic shock increases vascular permeability, leading to multiple organ failure and higher mortality. Reactive oxygen species (ROS) have been shown to promote both actin cytoskeleton reorganization resulting in vascular leakage and the formation of podosome, an actin-based dynamic membrane structure. Interestingly, recent studies have shown that circulating exosomes from septic patients contained higher levels of ROS than healthy ones. In this study, we hypothesized that septic exosomes can transfer exosomal ROS to endothelial cell (EC) to promote the generation of podosomes, leading to hyperpermeability. Methods: C57BL/6 mice (20-25g) were subjected to CLP surgery or injection with LPS (25 μg/g). Sham-operated or PBS-treated mice were used as controls. Exosomes were isolated from sera, collected at 3 h post-CLP or post-LPS-injection. Podosomes were identified by co-immunostaining F-actin/cortactin following stimulation with PMA, thrombin and exosomes. Transendothelial...

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