Cu Nanoparticles Have Different Impacts in Escherichia coli and Lactobacillus brevis than Their Microsized and Ionic Analogues (original) (raw)

SUPPORTING INFORMATION AVAILABLE Additional details relate to: Supplemental Materials and Methods, growth inhibition effects of Cu species in E. coli and L. brevis (Figure S1), individual growth inhibition curve including error bars for each of the Cu species in E. coli and L. brevis (Figure S2), area under growth-inhibition curve for Cu species in E. coli and L. brevis (Figure S3), comparison of regression lines for area under the growth inhibition curve as a function of % Cu dissolved in media for E. coli and L. brevis (Figure S4) schematic of sucrose gradient centrifugation procedure (Figure S5), standard curve of OD 600 and total number of cells (Figure S6), Cu bioavailability determined using bacterial biosensor strain (Figure S7), and confocal images of cells treated with n-FITC-CuO (Figure S8). Tables of data include: sources of Cu particles (Table S1), correlation coefficients for area under the growth inhibition curve and amounts of dissolved copper in media, cell-associated Cu, and Cu bioavailable in each of the bacterial species (Table S2), correlation between results from growth inhibition assays and results from the suite of sub-lethal assays (Table S3), and References for Supporting Information. This material is available free of charge via the Internet at http://pubs.acs.org.

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