The induction of apoptosis in HLECs or HBECs co-cultured with P. falciparum PRBCs depends on the culture conditions (original) (raw)

2014

Abstract

<p>A) Co-cultured endothelial cells (ECs) and parasitized red blood cells (PRBCs) after Annexin V and propidium iodide (PI) staining. B) Human lung endothelial cell (HLEC) apoptosis induced by the different <i>P. falciparum</i> isolates (A, B and C) when the co-cultures are made in the standard culture media (LCm) or in the modified culture medium (MLCm). C) Human brain endothelial cell (HBEC) apoptosis induced by the different <i>P. falciparum</i> isolates (A, B and C) when the co-cultures are made in the standard culture media (BCm) or in the modified culture medium (MBCm). The percentage of apoptotic cells was calculated after microscopy enumeration of Annexin V and propidium iodide positive cells in 1000 ECs. The results are expressed as mean values, and standard deviation are shown for duplicate wells from three biological replicates. Significance at <i>p</i><0.005 is indicated by **.</p

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