Biochemical Effects of Nano-Silicon Dioxide (SiO2) on Sunflower (Helianthus annuus L.) Plants: with SEM-EDX Analysis (original) (raw)

Nano-silicon protects sugar beet plants against water deficit stress by improving the antioxidant systems and compatible solutes

Acta Physiologiae Plantarum, 2020

Silicon (Si) can mitigate the deleterious impacts of various types of stresses on field crops. However, the potential of nanosilicon (nano-Si) in improving water stress and the relevant mechanisms remain unclear. Therefore, here, we examined the combined impacts of nano-Si and various irrigation regimes on antioxidant systems, osmolytes, photosynthesis-related parameters, and growth of sugar beet in a field trial. Treatments included three supplemental irrigation rates (I 1 , I 2 , and I 3) arranged based on the crop evapotranspiration (100% ET C , 75% ET C , and 50% ET C) and three doses of nano-Si: 0, 1, and 2 mM. Irrigation regime treatments were performed at the six-to eight-leaf stage (49 days after sowing), which continued until the harvest (180 days after sowing). Water stress brought about a detrimental impact on the sugar beet growth, the relative water content of leaves (LRWC), leaf area index (LAI), and photosynthetic performance. In contrast, Water deficiency enhanced hydrogen peroxide (H 2 O 2) and malondialdehyde (MDA) contents, which were followed by increasing antioxidant activities and osmolytes. Supplementation of nano-Si at low dose (1 mM) significantly increased chlorophyll contents, net photosynthesis (PN), glycine betaine (GB), flavonols (quercetin and rutin), and enzymatic antioxidants (superoxide dismutase, catalase, and guaiacol peroxidase). Furthermore, nano-Si at low dose (1 mM) decreased the amount of H 2 O 2 and MDA. Instead, the higher dose (2 mM) of nano-Si exerted toxic effects on severe water-stressed (50% ET C) plants. The parallel increase in MDA and proline contents in sugar beet plants treated with two mM nano-Si along with severe water stress supports the view that proline augmentation presumably is a sign of stress injury instead of stress resistance. Overall, our results imply that nano-Si can play a protecting role in sugar beet plants during water stress by enhancing antioxidants, GB, and flavonols (quercetin and rutin). However, the concentration of nano-Si must be chosen with care.

Biplot Analysis of Silicon Dioxide on Early Growth of Sunflower

Plant Breeding and Seed Science

Research into nanotechnology has advanced in almost all fields of technology and the aim of this study was to evaluate the role of nano-silicon dioxide (nano-SiO2) in germination performance sunflower. Germination and seedling growth are the most important stage of plant development and are critical factors to crop production and are essential to achieve optimum performance. The effects of pre-germination hydration in solutions of nano-SiO2 (0, 0.2, 0.4, 0.6, 0.8, 1 and 1.2 mM for 8 h) on germination characteristics of sunflower were investigated. The trait by treatment (TT) biplot explained 93% of the total variation of the standardized data (77% and 16% for the first and second principal components, respectively). According to polygon-view of TT biplot, T2 (0.2 mM) had the highest values for all of the measured traits except mean germination time and the time to 50% germination. The germination percentage was determined as the best trait and showed the high association with prompt...

Morpho-physiological and biochemical response of wheat to various treatments of silicon nano-particles under drought stress conditions

Scientific Reports

Silicon nanoparticles (Si-NPs) have shown their potential for use in farming under water-deficient conditions. Thus, the experiment was accomplished to explore the impacts of seed priming of Si-NPs on wheat (Triticum aestivum L.) growth and yield under different drought levels. The plants were grown in pots under natural ecological environmental conditions and were harvested on 25th of April, 2020. The results revealed that seed priming of Si-NPs (0, 300, 600, and 900 mg/L) suggestively improved, the spike length, grains per spike, 1000 grains weight, plant height, grain yield, and biological yield by 12–42%, 14–54%, 5–49%, 5–41%, 17–62%, and 21–64%, respectively, relative to the control. The Si-NPs improved the leaf gas trade ascribes and chlorophyll a and b concentrations, though decreased the oxidative pressure in leaves which was demonstrated by the diminished electrolyte leakage and upgrade in superoxide dismutase and peroxidase activities in leaf under Si-NPs remedies over the...

Silicon Nanoparticles Mitigate Hypoxia-Induced Oxidative Damage by Improving Antioxidants Activities and Concentration of Osmolytes in Southern Highbush Blueberry Plants

Agronomy

Climate change exacerbates flooding problems due to hurricanes followed by heavy rains, particularly in sub-tropical regions. Consequently, submerged plants experience hypoxia stress which limits agronomic and horticultural crop growth and production. Hypoxia causes oxidative damage by accelerating the lipid peroxidation associated with O2- and H2O2 levels. Additionally, hypoxia increases the accumulation of organic osmoprotectants and antioxidant activity, whereas it decreases the macronutrient (N, P, K, and Zn) uptake. This study aimed at investigating the effects of flooding-induced hypoxia stress on the growth and the physiological, biochemical, and nutritional characteristics of the hydroponically grown southern highbush blueberry (cv. Jewel) plants. In addition, the hypoxia-mitigating effects of conventional silicon (Si-C) and silicon nanoparticles (SiNPs) and their application methods (foliar vs. foliar and rootzone application) were also appraised. Both the Si-C and the SiNP...

Nano-silicon dioxide mitigates the adverse effects of salt stress on Cucurbita pepo L

Environmental Toxicology and Chemistry, 2014

Research into nanotechnology, an emerging science, has advanced in almost all fields of technology. The aim of the present study was to evaluate the role of nano-silicon dioxide (nano-SiO 2) in plant resistance to salt stress through improvement of the antioxidant system of squash (Cucurbita pepo L. cv. white bush marrow). Seeds treated with NaCl showed reduced germination percentage, vigor, length, and fresh and dry weights of the roots and shoots. However, nano-SiO 2 improved seed germination and growth characteristics by reducing malondialdehyde and hydrogen peroxide levels as well as electrolyte leakage. In addition, application of nano-SiO 2 reduced chlorophyll degradation and enhanced the net photosynthetic rate (P n), stomatal conductance (g s), transpiration rate, and water use efficiency. The increase in plant germination and growth characteristics through application of nano-SiO 2 might reflect a reduction in oxidative damage as a result of the expression of antioxidant enzymes, such as catalase, peroxidase, superoxide dismutase, glutathione reductase, and ascorbate peroxidase. These results indicate that nano-SiO 2 may improve defense mechanisms of plants against salt stress toxicity by augmenting the P n , g s , transpiration rate, water use efficiency, total chlorophyll, proline, and carbonic anhydrase activity in the leaves of plants.

Effect of SiO2 nanoparticles on phytochemical and anatomical alterations in Anthemis gilanica

2020

In this research, effects of SiO2 nanoparticles (NPs) on the growth, antioxidant properties, and phenolic and flavonoid contents were investigated in Anthemis gilanica plants from the Asteraceae family. Following seed germination, seedlings were cultured under Hoagland growth media and were treated with different concentrations of SiO2NPs (0, 2, 4, 6 and 8 g L-1). The results showed that SiO2 NPs significantly enhanced relative water content (RWC), dry and fresh weights, and shoot length especially at 4 g L-1, but decreased root length with increasing concentrations of NPs. Xylem number and size, and stele diameter increased up to 6 g L-1, and then decreased at higher concentration. Total phenol and flavonoid contents increased under different concentrations of SiO2 NPs, and the maximum content was observed at 6 g L-1. Moreover, SiO2 NPs increased antioxidant activity of extracts by reducing IC50 content especially at 6 g L-1. In conclusion, this study proposes that SiO2 NPs can imp...

Application of silicon nanoparticles in agriculture

3 Biotech

The beneficial effects of silicon and its role for plants are well established; however, the advantages of silicon nanoparticles over its bulk material are an area that is less explored. Silicon nanoparticles have distinctive physiological characteristics that allow them to enter plants and influence plant metabolic activities. The mesoporous nature of silicon nanoparticles also makes them good candidates as suitable nanocarriers for different molecules that may help in agriculture. Several studies have shown the importance of silicon nanoparticles in agriculture, but an overview of the related aspects was missing. Therefore, this review brings together the literature on silicon nanoparticles and discusses the impact of silicon nanoparticles on several aspects of agricultural sciences. The review also discusses the future application of silicon nanoparticles in plant growth, plant development, and improvement of plant productivity.

Silicon Nanoparticle-Induced Regulation of Carbohydrate Metabolism, Photosynthesis, and ROS Homeostasis in Solanum lycopersicum Subjected to Salinity Stress

ACS Omega

Agricultural crops are facing major restraints with the rapid augmentation of global warming, salt being a major factor affecting productivity. Tomato (Solanum lycopersicum) plant has immense nutritional significance; however, it can be negatively influenced by salinity stress. Nanoparticles (NPs) have excellent properties, due to which these particles are used in agriculture to enhance various growth parameters even in the presence of abiotic stresses. The objective of this study was to investigate the effects of silicon NPs (Si-NPs) through root dipping and foliar spray on tomato in the presence/absence of salt stress. Plant root and leaf were used for the measurements of morphological, physiological, and biochemical parameters treated with Si-NPs under salt stress. At 45 days after sowing, the activity of antioxidant enzymes, photosynthesis, mineral concentration, chlorophyll index, and growth attributes of tomato plants were measured. The developmental processes of tomato plants were severely slowed down by salt stress upto 35.8% (shoot dry mass), 44.3% (root dry mass), 51% (shoot length), and 62% (root length), but this reduction was mitigated by the treatment of Si-NPs. Application of Si-NPs significantly increased the growth attributes (height and dry weight), mineral content [magnesium (Mg), potassium (K), copper (Cu), iron (Fe), manganese (Mn), zinc (Zn)], photosynthesis [net photosynthetic rate (P N), stomatal conductance (gs), transpiration rate (E), internal CO 2 concentration (Ci)], and activity of antioxidative enzymes including superoxide dismutase and catalase in salt stress. Foliar application of Si-NPs in tomato plants appears to be more effective over root dipping and alleviates the salt stress by increasing the plant's antioxidant enzyme activity.

Green Synthesis of SiO 2 NPs Promote Vigna radiata L Germination, Membrane Damage, Antioxidant enzymes and Physiological activities

In agriculture sector demand for nanotechnology-based products in current years, have led to the increasing use of nanoparticles (NPs). Biologically prepared NPs have gained a lot of attraction due to ecofriendly, less expensive and biocompatible process, therefore, Current work was design to investigate the green synthesized SiO2 NPs on Vigna radiata L. physiological and biochemical analysis. Six days old plants (two leaf stage) exposed to SiO2 NPs at 2, 20, 200 and 2000 ppm. Significantly increased in plant length and weight at on all concentrations at different intervals except 20 ppm of SiO2 NPs reduced plant length and weight. However, the antioxidant enzymes at 20 ppm treated plant showed several effects. The production of ascorbate peroxidase and catalase increased as compared to control. Peroxidase level was initially increased but then reduced later, superoxide dismutase also reduced as compared to control. FT-IR at 20 ppm treated plant exhibited the increase in protein, de...