Bioassay Guided Fractionation Protocol for Determining Novel Active Compounds in Selected Australian Flora (original) (raw)

LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential

Antioxidants, 2021

Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize and quantify the phenolic compounds from 10 widely used Australian-grown herbs (oregano, rosemary, bay, basil, sage, fenugreek, dill, parsley, mint and thyme). For this purpose, liquid chromatography mass spectrometry (LC-MS) was used for the complete profiling of polyphenolic compounds and quantification of abundant phenolic compounds was completed with high-performance liquid chromatography—photodiode array detection (HPLC-PDA). Polyphenols from Australian-grown herbs were estimated through total phenolic content (TP), total flavonoids (TF) and total tannins (TT) along with their in-vitro antioxidant activities. Oregano and mint were estimated with the highest value of TP (140.59 ± 9.5...

Review of the methods used for isolating pharmaceutical lead compounds from traditional medicinal plants

The Environmentalist, 2007

The possibility of finding new medicines from natural sources is one of the more commonly cited reasons for preserving biodiversity, and employing indigenous knowledge of traditional healing remedies greatly increases the likelihood of discovering these hidden medicinal compounds. The main difficulties in using natural products as a source for pharmaceutical lead compounds involve separating the plethora of compounds from the original extract, as well as the gamble of time and money invested in an activity that may not yield a novel compound. However, while these difficulties exist, the potential of natural products still far outweighs the limitations of the simple structures and known modes of action of synthetic lead compounds. As such, the production of novel medicines, particularly for cancer and human immunodeficiency virus (HIV) treatments as well as the inhibition of antibiotic-resistant bacteria, now requires the utilization of natural products. This paper provides a review of the current methods used in elucidating pharmaceutical lead compounds from natural sources, focusing on plant samples in particular.

Qualitative Phytochemical Screening of Three Indigenous Medicinal Plants

2013

Many modern medicines had their origin in medicinal plants. The present study was undertaken in three common plants viz; Mangifera indica (Mango), Azadiracta indica (Neem) and Lantana camara which are found to have anti diabetic and other medicinal properties. Pytochemical analyses of the active components of the plants by using different solvents were carried out. The fresh leaves of mango, neem and lantana were collected from the campus of Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Tathawade, Pune. Methanol, petroleum ether and water extracts of fresh and dry leaves of all the three plants were obtained by standard procedure. Filtered extracts were concentrated and were subjected to qualitative phytochemical analysis for various secondary metabolites like alkaloids, glycosides, steroids, triterpenoids, tannins, saponins and flavonoids. Phytochemical analysis indicated the presence of these constituents in these plants whereas variable results were observed with di...

Potential of bioactive compounds derived from underutilized fruit-bearing plants: a comprehensive review

European Food Research and Technology, 2022

In nature, varieties of underutilized fruit-bearing plants are available and have remained underexploited for various reasons. However, the different parts of the plant, mainly the fruit, contributed considerably towards the sustainability of food as abundant sources of imperative phytochemical compounds and possess the potential for revenue generation and the conservation of ecological stability. Ethnobotanical information regarding underutilized plants was acquired from a literature exploration of diverse databases for instance Scopus, Google Scholar, and PubMed up to 2020 from research publications. This review article offers an inclusive summary of about 14 underutilized plants, which are supported through experimental evidence, either in vitro or else in vivo. Bioactive compounds such as the secondary plant metabolites phytochemicals and nutrients available in these underutilized plant parts, such as fruits, leaves, and bark, explain their potential applications in different kinds of industries including mainly those of food and pharmaceutical products. In this sense, the phytonutrient significance, biological activities, or possible mechanistic health-related aspects of these compounds are addressed in this review. Based on the accessible indication on the species' safety and pharmacology, we highlighted diverse ways wherein the therapeutic potential effects against different diseases of underutilized plant parts could be appropriately harnessed for probable incorporation into the country's healthcare structure. This study concluded that cited underutilized plants are an immense source of phytochemicals providing diverse antioxidant and other biological activities, viz: anti-inflammatory, antimicrobial, antioxidant, antidiabetic, chemopreventive, and antiallergic, and hence, they can be exploited as alternative sources of therapeutic bioactive compounds for various pharmaceutical applications.

Chemical prospecting of medicinal plants for drug discovery

2010

There is no island that can exist without the support of other living and non living things therefore I am most grateful to all for making this study a success: • My God, for His mercies that endures forever. Lord you have highly favored me from my birth till date and l can't but live for you all the days of my life.

Phytochemical Study and Anticancer Potential of High Antioxidant Australian Native Plants

2018

High antioxidant capacities have been linked with a reduction in the incidence of chronic diseases including cardiovascular disease, diabetes, obesity, neurodegeneration, inflammation and cancer. Furthermore, phenolic antioxidants may not only have protective effects against these diseases, but may also have therapeutic potential in reversing them by modulation of the cellular redox state. Thus, plants with high antioxidant capacities have potential in the development of new chemotherapeutic treatments to prevent and treat some cancers. Several native Australian plant species including Terminalia ferdinandiana Exell (Kakadu plum), Tasmannia lanceolata (Poir.) A.C.Sm., (Tasmannian pepper), Syzygium australe (H.L.Wendl. ex Link) B.Hyland (brush cherry), Syzygium luehmannii (F.Muell.) L.A.S.Johnson (riberry), Davidsonia pruriens F.Muell. (Davidson's plum), Elaeocarpus angustifolius Blume (quandong), Kunzea pomifera F.Muell. (muntries), Podocarpus elatus R.Br.ex Endl., (Illawarra pl...