Cyanoboletus macroporus (Boletaceae), a new bolete species from Pakistani forests Samina (original) (raw)
Phytotaxa, 2019
Hortiboletus kohistanensis is described as a new species from the moist temperate and subalpine oak forests of Pakistan after detailed morphological and phylogenetic analyses as well as comparison with related taxa. This oak-associated species is morphologically distinguished from allied taxa by an areolate pileus surface and whitish stipe base. It is also phylogenetically distinct from H. indorubellus, the most closely related species, from India.
Newly recorded species of boletaceae form Pakistan
Pakistan Journal of Botany
Family Boletaceae is characterized by tubular hymenium that contains simple/compound, minute, broad or hexagonal tubes that some times may be radial, elongated or nearly gill-like, and mostly fusiform or elongated spores. The present describes 5 new records of the members of the family Boletaceae viz., Aureoboletus gentilis, Boletus reticulatus, B. edulis, B. subtomentosus and Chalciporus piperatus from Gilgit-Baltistan. Of these, A. gentilis, B. reticulatus and B. subtomentosus are reported for the first time from Pakistan.
Mycosphere, 2021
The Boletaceae is the largest family of fleshy fungi in the Boletales. Despite the extensive history of work in the Boletaceae in North America, novel species and genera are continually being described. Multigene molecular phylogenetic analyses of five loci were combined with thorough morphological studies to investigate the taxonomy of several boletes from the southeastern USA. Based on our results, we describe four new species: Aureoboletus pseudoauriporus, Cyanoboletus bessettei, Hemileccinum floridanum, and Xerocomellus bolinii. We also propose three combinations to reflect the results of our molecular analyses: Cyanoboletus cyaneitinctus comb. nov., a bolete that is widespread across the eastern USA, C. cyaneitinctus f. reticulatus, and Lanmaoa sublurida, a rarely-documented bolete that is so far known only from Florida. Keywords-Boletalesectomycorrhizalphylogeny Materials & Methods Sampling and morphological studies Specimens were collected in situ between 2015-2020 and deposited at the University of South Florida Herbarium (USF). Additional collections were obtained on loan from Florida Museum of Natural History (FLAS) for study. Macroscopic descriptions were made using fresh basidiomes. Micromorphological features were observed with a phase contrast microscope (AmScope, Irvine, CA, USA). Distilled H2O, lactoglycerol, KOH, and Phloxine B were used to rehydrate and stain sections (Singer 1986). Measurements were made at 1000 × with a calibrated ocular micrometer in Piximètre 5.9 R 1532 (http://piximetre.fr). Basidiospore dimensions are reported as length by width, with each measurement reported as the minimum, the average minus the standard deviation, the average plus the standard deviation, and the maximum. Spore dimensions are followed by the number of spores counted, N, and the average quotient mean, Q, where Q is the average length divided by the average width. Scanning Electron Microscopy (SEM) was performed at the Electron Microscopy Core Facility at the University of South Florida on an Aquila Hybrid Scanning Electron Microscope (Topcon, Tokyo, Japan). DNA Extraction, PCR amplification, and sequencing Genomic DNA was isolated as described in Farid et al. (2017). A subset of the samples was 1045 Results Phylogenetic analyses The final dataset consisted of 305 specimens comprising 141 ITS, 234 28S, 140 RPB1, 165 RPB2, 216 TEF1 sequences (Supplementary Table 1). A total of 143 sequences were generated for this study. The six species from this study were distributed across five genera. One species of Aureoboletus forms a strongly supported clade (0.96 BPP, 96 bootstrap replicate support), with somewhat strong support (0.91 BPP, 94 bootstrap replicate support) as a sister clade with Aureoboletus auriporus (Peck) Pouzar. A strongly supported clade in Hemileccinum with somewhat strong support (0.96 BPP, but <70 bootstrap replicate support) was sister to a clade of Hemileccinum subglabripes (Peck) Halling. In Xerocomellus, a strongly supported clade was sister to an unnamed Xerocomellus sp. (HKAS 56311) from China. Three specimens of Nigroboletus roseonigrescens Gelardi, Vizzini, E. Horak, T.H. Li & Ming were strongly supported as basal to all Xerocomellus sequences included in the analyses. Two species in Cyanoboletus were recovered in the analyses. This first Cyanoboletus species is in a strongly supported clade (1 BPP, 98 bootstrap replicate support) containing Cyanoboletus pulverulentus s.str., and Cyanoboletus sinopulverulentus (Gelardi & Vizzini) Gelardi, Vizzini & Simonini, although C. sinopulverulentus did not receive strong support as sister to either of these species. The second Cyanoboletus species formed a strongly supported sister clade to an unnamed Cyanoboletus sp. (HKAS 76850) from China, and a clade containing Cyanoboletus instabilis (W.F. Chiu) G. Wu & Zhu L. Yang. A species of Lanmaoa formed a strongly supported sister clade to Lanmaoa roseocrispans A.E. Bessette, A.R. Bessette, Nuhn & Halling. Pulchroboletus rubricitrinus (Murrill) Farid & A.R. Franck, which was strongly supported as a sister clade to Pulchroboletus roseoalbidus (Alessio & Littini) Gelardi, Vizzini & Simonini, was consistent with the results from the nucDNA analysis in Farid et al. (2017). Our collection of Exsudoporus floridanus formed a strongly supported clade with Exsudoporus floridanus from Belize (1.0 BPP, 100 bootstrap replicate support), while the Exsudoporus clade was strongly supported as sister to Butyriboletus (1.0 BPP, 0.96 bootstrap replicate support).
PloS one, 2015
We describe and illustrate two new species of Boletellus section Boletellus, B. aurocontextus sp. nov. and B. areolatus sp. nov., which are generally assumed to be B. emodensis. In this study, we reconstructed separate molecular phylogenetic trees of section Boletellus using the nucleotide sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA, the largest subunit (RPB1) and the second-largest subunit (RPB2) of nuclear RNA polymerase II gene and mitochondrial cytochrome oxidase subunit 3 (cox3) gene. We also examined the morphologies of B. emodensis sensu lato (s.l.) and other related species for comparison. The molecular phylogenetic tree inferred from the sequences of nuclear DNA (ITS, and combined dataset of RPB1 and RPB2) indicated that three genetically and phylogenetically well-separated lineages were present within B. emodensis s.l. These three lineages were also distinguished on the basis of the molecular phylogenetic tree constructed using the se...
Plant Syst Evol, 2004
A molecular phylogenetic analysis of Cynoglottis was performed to evaluate previous hypotheses based on non-molecular evidence concerning the position of this genus within Boraginaceae tribe Boragineae. ITS-5.8S and trnL UAA sequences from the nuclear and chloroplast noncoding genomes were obtained for four Cynoglottis taxa and selected members of the related genera Anchusa, Anchusella, Gastrocotyle, Brunnera and Pentaglottis. Cynoglottis is monophyletic, but neither trnL nor ITS support a close relationship with Brunnera, unlike previously supposed on morphological grounds. Brunnera is, instead, related to the southwestern European monotypic genus Pentaglottis, with which it forms a basal clade. ITS-5.8S sequences show that Anchusa thessala, a southeastern European annual species of Anchusa subg. Buglossellum, is sister to Cynoglottis and that the two taxa form a clade which also includes the Balkan endemic Gastrocotyle macedonica. Species of Anchusa subg. Anchusa form a separate lineage with high bootstrap support, suggesting that this heterogeneous genus is paraphyletic with respect to Cynoglottis. ITS sequences also discriminate between the Balkan-Apenninic diploid C. barrelieri and the Anatolian tetraploid C. chetikiana, albeit with low support. The molecular results are discussed in the light of karyological, morphological and chorological aspects.
Binderoboletus segoi gen. and sp. nov., Guya-naporus albipodus gen. and sp. nov. and Singerocomus rubriflavus gen. and sp. nov. (Boletaceae, Boletales, Basidiomycota) are described from the Pakaraima Mountains and adjacent lowlands of Guyana. Xeroco-mus inundabilis, originally described from the central Brazilian Amazon and based solely on the type collection , is redescribed from numerous collections from Guyana and transferred into Singerocomus. These boletes occur in Neotropical forests dominated by ectomycorrhizal trees in the genera Dicymbe (Faba-ceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae) and Pakaraimaea (Diptero-carpaceae). Three of the species were repeatedly found in a multiyear sporocarp survey in Dicymbe cor-ymbosa-monodominant forest. Macromorphological, micromorphological, habitat and multilocus DNA sequence data are provided for each species. A molecular phylogenetic analysis based on a large taxon set across the Boletaceae justifies erection of the new genera.
Tylopilus himalayanus and T. pseudoballoui are described as new species from two Himalayan states (Sikkim and Uttarakhand) in India. Tylopilus himalayanus is characterised by a unique combination of features: reddish-or brownish-grey to purplish-grey then brown to reddish-brown or darker pileus, absence of olive or violet tinges on stipe surface, angular pores, stipe without reticulum or rarely with a faint reticulum restricted to the very apex, bitter taste of the context and positive macrochemical colour reaction of the stipe context with KOH (dark orange) and FeSO 4 (dark green), medium sized (10.9-14.4 × 3.9-4.9 µm) basidiospores and occurrence under coniferous trees; T. pseudoballoui is distinguished by orange-yellow to brown-yellow sticky pileus, pale yellow pore surface with pinkish hues that turns pale to greyish-orange on bruising; angular pores, stipe concolorous to pileus with pruinose but never reticulate surface, ixocutis pattern of pileipellis and occurrence under broadleaf trees. Another species, T. neofelleus, which was reported earlier from China and Japan, was also collected from Sikkim and reported for the first time from India. All three species are described with morphological details and two-locus based (nrLSU and nrITS) phylogenetic data.
Tylopilus himalayanus and T. pseudoballoui are described as new species from two Himalayan states (Sikkim and Uttarakhand) in India. Tylopilus himalayanus is characterised by a unique combination of features: reddish-or brownish-grey to purplish-grey then brown to reddish-brown or darker pileus, absence of olive or violet tinges on stipe surface, angular pores, stipe without reticulum or rarely with a faint reticulum restricted to the very apex, bitter taste of the context and positive macrochemical colour reaction of the stipe context with KOH (dark orange) and FeSO 4 (dark green), medium sized (10.9-14.4 × 3.9-4.9 µm) basidiospores and occurrence under coniferous trees; T. pseudoballoui is distinguished by orange-yellow to brown-yellow sticky pileus, pale yellow pore surface with pinkish hues that turns pale to greyish-orange on bruising; angular pores, stipe concolorous to pileus with pruinose but never reticulate surface, ixocutis pattern of pileipellis and occurrence under broadleaf trees. Another species, T. neofelleus, which was reported earlier from China and Japan, was also collected from Sikkim and reported for the first time from India. All three species are described with morphological details and two-locus based (nrLSU and nrITS) phylogenetic data.
A new species of Boletellus (Boletaceae, Basidiomycota) from tropical India
Nordic Journal of Botany, 2018
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as
Descolea quercina (Bolbitiaceae), a new species from moist temperate forests in Pakistan
MycoKeys, 2017
A new species, Descolea quercina, is described and illustrated from Northern parts of Khyber Pakhtunkhwa, Pakistan. It is characterized by medium to large basidiomata, squamose to squamosegranulose hygrophanous pileus, and limoniform, verrucose basidiospores with partly concrescent verrucae. Phylogenetic analyses of nuc rDNA region encompassing the internal transcribed spacers 1 and 2 along with 5.8S rDNA (ITS) and nuc 28S rDNA D1-D2 domains (28S) also confirmed it as a new species. A comparison with similar taxa is provided.