A Comparative Analysis of the Effect of two Mouthrinses on the Accumulation of Biofilm on Dentures (original) (raw)
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Comparative Analysis of Different Chemical Methods for Removing Biofilm from Complete Dentures
Journal of Dentistry, Oral Disorders & Therapy
The aim of this study was to make a comparative evaluation of three chemical substances for cleaning complete dentures, as regards their efficacy of biofilm removal. The sample consisted of 20 maxillary complete dentures. The dentures were randomly divided into groups according to the chemical cleaning method to be used: Group 1-water (control), Group 2-sodium hypochlorite solution, Group 3-sodium perborate (Corega Tabs®) and Group 4-2% chlorhexidine. The groups were evaluated in terms of the quantity of biofilm before and after application of the chemical cleaning method by applying a revealer, and later, analysed by the Denture Hygiene Index (DHI). The results showed that only sodium hypochlorite solution was effective for biofilm removal. There was statistically significant difference among the groups, pointing out greater efficacy of the method used in Group 2 in comparison with Groups 1 and 4. Group 3 did not differ statistically from any other group evaluated. The results allowed to conclude that sodium hypochlorite solution is the most efficient chemical agent for removing biofilm from complete dentures. However, when used alone, all the tested chemical cleaning methods were incapable of eliminating all the biofilm from denture surfaces.
The International journal of prosthodontics, 2021
PURPOSE To evaluate the effects of 0.2% sodium hypochlorite, Efferdent (Prestige Consumer Healthcare), and 6.25% Ricinus communis on biofilm removal and antimicrobial action on dentures and brushes using nonimmersion or immersion protocols for the brushes. MATERIALS AND METHODS A total of 45 denture wearers were randomly assigned to a denture immersion protocol for 7 days: 0.85% saline solution for 20 minutes (control); 0.2% sodium hypochlorite for 20 minutes (SH); Efferdent for 3 minutes; or 6.25% Ricinus communis for 20 minutes (RC). The participants were also randomized to immersion (n = 23) or no immersion (n = 22) of their brushes with their dentures in the same solutions. For biofilm evaluation, the dentures were stained and photographed, and the area of the biofilm was measured using Image Tool 3.0 (University of Texas Health Science Center). To evaluate microbial load on dentures and brushes, the biofilm was collected, and the Candida spp and Streptococcus mutans colonies we...
Effect of Chlorhexidine on Denture Biofilm Accumulation
Journal of …, 2012
Purpose: Adequate denture hygiene can prevent and treat infection in edentulous patients, who are frequently elderly and have difficulty brushing their teeth. This study evaluated the efficacy of complete denture biofilm removal using a chlorhexidine solution in two concentrations: 0.12% and 2.0%. Materials and Methods: Sixty complete denture wearers participated in a trial for 21 days after receiving brushing instructions. They were distributed into three groups, according to the tested solution and regimen (n = 20): (G1) Control (daily overnight soaking in water); (G2) daily immersion at home in 0.12% chlorhexidine for 20 minutes after dinner; and (G3) a single immersion in 2.0% chlorhexidine for 5 minutes at the end of the experimental period, performed by a professional. Biofilm coverage area (%) was quantified on the internal surface of maxillary dentures at baseline and after 21 days. Afterward, the differences between initial and posttreatment results were compared by means of the Kruskal-Wallis test (α = 0.05). Results: Median values for biofilm coverage area after treatment were: (G1) 36.0%; (G2) 5.3%; and (G3) 1.4%. Differences were significant (KW = 35.25; p < 0.001), although G2 and G3 presented similar efficacy in terms of biofilm removal. Conclusions: Both chlorhexidine-based treatments had a similar ability to remove denture biofilm. Immersion in 0.12% or 2.0% chlorhexidine solutions can be used as an auxiliary method for cleaning complete dentures.
PLOS ONE, 2016
Background Appropriate oral hygiene is required to maintain oral health in denture wearers. This study aims to compare the role of denture cleaning methods in combination with overnight storage conditions on biofilm mass and composition on acrylic removable dentures. Methods In a cross-over randomized controlled trial in 13 older people, 4 conditions with 2 different mechanical cleaning methods and 2 overnight storage conditions were considered: (i) brushing and immersion in water without a cleansing tablet, (ii) brushing and immersion in water with a cleansing tablet, (iii) ultrasonic cleaning and immersion in water without a cleansing tablet, and (iv) ultrasonic cleaning and immersion in water with a cleansing tablet. Each test condition was performed for 5 consecutive days, preceded by a 2-days wash-out period. Biofilm samples were taken at baseline (control) and at the end of each test period from a standardized region. Total and individual levels of selected oral bacteria (n = 20), and of Candida albicans were identified using the Polymerase Chain Reaction (PCR) technique. Denture biofilm coverage was scored using an analogue denture plaque score. Paired t-tests and Wilcoxon-signed rank tests were used to compare the test conditions. The level of significance was set at α< 5%. Results Overnight denture storage in water with a cleansing tablet significantly reduced the total bacterial count (p<0.01). The difference in total bacterial level between the two mechanical cleaning methods was not statistically significant. No significant effect was observed on the amount of Candida albicans nor on the analogue plaque scores.
Efficacy of three denture brushes on biofilm removal from complete dentures
Journal of Applied Oral Science, 2007
he aim of this study was to compare the efficacy of three denture brushes (Bitufo-B; Medic Denture-MD; Colgate-C) on biofilm removal from upper and lower dentures using a specific dentifrice (Corega Brite). The correlation between biofilm levels on the internal and external surfaces of the upper and lower dentures was also evaluated. A microbiological assay was performed to assess the growth of colony-formed units (cfu) of Candida yeasts on denture surface. Thirty-three patients were enrolled in a 10-week trial divided in two stages: 1 (control)-three daily water rinses within 1 week; 2-three daily brushings within 3 weeks per tested brush. Internal (tissue) and external (right buccal flange) surfaces of the complete dentures were disclosed (neutral red 1%) and photographed. Total denture areas and disclosed biofilm areas were measured using Image Tool 3.00 software for biofilm quantification. Dentures were boxed with #7 wax and culture medium (CHROMagar TM Candida) was poured to reproduce the internal surface. Statistical analysis by Friedman's test showed significant difference (p<0.01) between control and brushing stages. No difference was found among the brushes with respect to their efficacy on biofilm removal (p>0.01). Analysis by the Correlation test showed higher r values (B=0.78; MD=0.8341, C=0.7362) for the lower dentures comparing the surfaces (internal and external) and higher r values (B=0.7861, MD=0.7955, C=0.8298) for the external surface comparing the dentures (upper and lower). The results of the microbiological showed no significant difference (p>0.01) between the brushes with respect to the frequency of the species of yeasts (chi-square test). In conclusion, all denture brushes evaluated in this study were effective in the removal of biofilm. There was better correlation of biofilm levels between the surfaces for the lower dentures, and between the dentures for the external surface. There was no significant difference among the brushes regarding the frequency of yeasts.
Brazilian Dental Journal, 2013
This study compared the levels of biofilm in maxillary and mandibular complete dentures and evaluated the number of colony-forming units (cfu) of yeasts, after using auxiliary brushing agents and artificial saliva. Twenty-three denture wearers with hyposalivation and xerostomia were instructed to brush the dentures 3 times a day during 3 weeks with the following products: Corega Brite denture dentifrice, neutral liquid soap, Corega Brite combined with Oral Balance (artificial saliva) or tap water. For biofilm quantification, the internal surfaces of the dentures were disclosed, photographed and measured using a software. For microbiological analysis, the biofilm was scrapped off, and the harvested material was diluted, sown in CHROMagar™ Candida and incubated at 37°C for 48 h. Data were analyzed statistically by two-way ANOVA and Tukey's test (α=0.05). Mandibular dentures presented a mean biofilm percentage (µ=26.90 ± 21.10) significantly greater than the maxillary ones (µ=18.0 ...
Evaluation of three indices for biofilm accumulation on complete dentures
Gerodontology, 2010
Evaluation of three indices for biofilm accumulation on complete dentures Objectives: The objective of this study was to evaluate the accuracy and reproducibility of three complete denture biofilm indices (Prosthesis Hygiene Index; Jeganathan et al. Index; Budtz-Jørgensen Index) by means of a computerised comparison method. Background: Clinical studies into denture hygiene have employed a large number of biofilm indices among their outcome variables. However, the knowledge about the validity of these indices is still scarce. Materials and methods: Sixty-two complete denture wearers were selected. The internal surfaces of the upper complete dentures were stained (5% erythrosine) and photographed. The slides were projected on paper, and the biofilm indices were applied over the photos by means of a scoring method. For the computerised method, the areas (total and biofilm-covered) were measured by dedicated software (Image Tool). In addition, to compare the results of the computerised method and Prosthetic Hygiene Index, a new scoring scale (including four and five graded) was introduced. For the Jeganathan et al. and Budtz-Jørgensen indices, the original scales were used. Values for each index were compared with the computerised method by the Friedman test. Their reproducibility was measured by means of weighed j. Significance for both tests was set at 0.05. Results: The indices tested provided similar mean measures but they tended to overestimate biofilm coverage when compared with the computerised method (p < 0.001). Agreement between the Prosthesis Hygiene Index and the computerised method was not significant, regardless of the scale used. Jeghanathan et al. Index showed weak agreement, and consistent results were found for Budtz-Jorgensen Index (j = 0.19 and 0.39 respectively). Conclusion: Assessment of accuracy for the biofilm indices showed instrument bias that was similar among the tested methods. Weak inter-instrument reproducibility was found for the indices, except for the Budtz-Jørgensen Index. This should be the method of choice for clinical studies when more sophisticated approaches are not possible.
Gerodontology, 2007
Comparative analysis of biofilm levels in complete upper and lower dentures after brushing associated with specific denture paste and neutral soap Objectives: The objective of this study was to compare and correlate biofilm levels in complete upper and lower prosthesis after brushing, associated with specific paste and soap, by means of computerised methodology. Materials and methods: Forty-five complete denture wearers were selected and instructed to brush their prostheses (Soft Oral B 40) three times a day for 3 weeks with water (Control), specific paste for complete dentures (Corega Brite) (Experiment 1) and neutral soap (Experiment 2). The study was based on a crossover model and a wash-out period was not included. For biofilm quantification, the internal surfaces were dyed (neutral red 1%), photographed (Canon EOS Digital) and the disclosed biofilm was measured with the Image Tool 2.0 software. The products were assessed by means of a questionnaire regarding their hygiene properties and acceptance. Results: The variance analysis indicated that the lower prostheses exhibited a mean biofilm percentage, significantly higher than the upper prostheses and that brushing with paste (Experiment 1) was more effective than soap (Experiment 2) and, in turn, this was more effective than water (Control). There was a high biofilm correlation (Pearson correlation) between both prostheses. Both products were well accepted by the patients, but the most favoured one was the paste. Conclusions: This was effective in controlling the biofilm and can be used preventatively in the maintenance of oral health by wearers of complete dentures. This is important where the lower prosthesis can harbour microorganisms which may act as a reservoir for other areas of the mouth and thus enhance the importance of proper hygiene.
BMC Oral Health
Background The continuously increasing demand for removable denture appliances and the importance of adequate denture cleaning have led to the development of various denture cleansing products. The aim of this study was to evaluate the efficacy of two novel denture cleansing agents (GE and TM) and three commonly available cleansers (0.5% sodium hypochlorite; NaClO, 0.12% chlorhexidine gluconate; CHX, and Polident®; POL) on multispecies microbial biofilm formation, stain removal and physical properties of dentures. Methods The antimicrobial activities of denture cleansing agents were determined against major oral opportunistic pathogens including Streptococcus mutans, Staphylococcus aureus, Escherichia coli and Candida albicans, using time-kill assays. Multispecies microbial biofilms grown on acrylic resins for 72 h were generated to determine the antibiofilm effects of cleansing agents by confocal laser scanning microscopy (CLSM). Evaluations of the tea and coffee stain removal prop...