Protective effect of Nigella sativa oil against tramadol-induced tolerance and dependence in mice: Role of nitric oxide and oxidative stress (original) (raw)

The present study was designed to investigate the adverse reproductive effects of acetamiprid, besides the possible protective role of Nigella sativa oil (NSO), as a potential antioxidant agent. Thirty-two male Wistar rats were allocated into four equal groups of eight, control (CRL), acetamiprid (ACMP, 27 mg/kg), Nigella sativa oil (NSO, 0.5 ml/kg) and in combination (ACMP þ NSO). The experimental animals were dosed by gavage (5 days per week) for 45 consecutive days. Body weight gain, reproductive organs weights, sperm characteristics, testosterone, and thiobarbutiric acid-reactive substances (TBARS) levels were investigated. The obtained results showed that ACMP decreased significantly (p < 0.001) the body weight gain and the absolute weights of reproductive organs (testes, epididymis, and seminal vesicles). Furthermore, significant alterations at least (p < 0.01) in semen characteristics were noted in ACMP group as evidenced by a decline in spermatids number, sperm count, sperm motility, and testosterone level with an increase in abnormal and dead sperm and TBARS level. Treatment with NSO alone may stimulate spermatogenesis, increased significantly (p < 0.001) spermatids number and the weight of seminal vesicles. On the other hand, the co-administration of NSO along with ACMP can mitigate more efficiently and modulate in certain cases the adverse effects induced by ACMP on reproductive organs weights, semen quality, testosterone, and TBARS levels (at least p < 0.001). This obvious protective role of NSO against ACMP induced reproductive toxicity may be due to its antioxidant properties and ability to reduce TBARS levels as shown in this work.