Hemoglobin aggregates studied under static and dynamic conditions involving the formation of nanobacteria-like structures (original) (raw)
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2019
This open access document is published as a preprint in the Beilstein Archives with doi: 10.3762/bxiv.2019.112.v1 and is considered to be an early communication for feedback before peer review. Before citing this document, please check if a final, peer-reviewed version has been published in the Beilstein Journal of Nanotechnology. This document is not formatted, has not undergone copyediting or typesetting, and may contain errors, unsubstantiated scientific claims or preliminary data.
Previously we have established that a reductionist model of the erythrocyte (red blood cell; RBC) suitable for the interpretation of small angle neutron scattering data is a concentrated solution (~30% volume fraction) of haemoglobin represented as hard spheres, and that this solution is far from thermodynamically ideal by virtue of the self-association of the spheres. The study will examine the effect of confinement, the RBC plasma membrane, on the nanoscale-structure of concentrated solutions of free haemoglobin (Hb_4), haemoglobin with bound carbon monoxide (Hb_4-CO), and haemoglobin bound with bound oxygen (Hb_4-O2), using a combination of rheological measurements and small angle neutron scattering (SANS) on whole RBCs and concentrated solutions of cell-free haemoglobin.