Fast Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Low Concentration of Seminyak (Champeria sp.) Leaf Extract (original) (raw)
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Indonesian Journal of Chemistry
Silver nanoparticles (AgNPs) are fascinating materials for biomedical applications thanks to their strong antibacterial activity and biocompatibility. This study applied the green synthesis method using 0.5 wt.% Seminyak leaf extract and assisted with one min microwave irradiation to enhance AgNPs formation. Extremely small sizes AgNPs with an average particle size of 9.1 ± 4.1 nm and spherical shapes were obtained. The synthesized AgNPs displayed potent antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria with a zone of inhibition of 12.3 ± 0.1 and 13.7 ± 0.7 mm, respectively. The MTT assay results demonstrated that the cells’ viability of the obtained AgNPs was 88.5 ± 7.0 %, implying biocompatibility for biomedical applications.
Journal of Chemical and Petroleum Engineering, 2024
Silver nanoparticles (Ag NPs) as a new antibiotic generation were green produced using Arabic gum, as capping and stabilizing agents, under microwave heating. Results indicated that using 0.5 mL of 3 mM silver nitrate solution and 0.5 mL of Arabic gum solution (1 % W/V), and microwave heating time of 150 s, Ag NPs were fabricated minimum broad emission peak (λmax) and maximum concentration of 424±2 nm and 25±2 ppm, respectively. Transmission electron microscopy and dynamic light scattering analyses specified that the fabricated spherical Ag NPs using these optimal synthetic parameters had particle size, polydispersity index and zeta potential values of the 89 nm, 0.238 and +50 mV. Furthermore, antibacterial test indicated that diameters of the formed clear zones around the holes having Ag NPs were 13 and 15 mm, toward Escherichia coli and Staphilococcus aurous, respectively. Antifungal assessment also shown that synthesized Ag NPs could strongly inhibit the growth of Aspergillus flavus mycelia in the plate during incubation for 7 days. Synthesized Ag NPs using the obtained optimum conditions can be widely used in the food, pharmaceutical and cosmetics areas, due to those high antimicrobial activities.
Crystals
Herein, a simple one-step microwave irradiation technique has been used to synthesize the silver nanoparticles using silver nitrate (AgNO3) solution and cetyltrimethyl ammonium bromide (CTAB) as a stabilizing agent. The crystals of as-prepared nanoparticles were studied using X-ray diffraction (XRD) and a selected area electron diffraction (SAED) pattern, confirming the single-phase face-centered cubic structure. The optical property measured using UV-Vis spectroscopy shows an absorption maximum at 420 nm, which also confirms the formation of silver nanoparticles. Transmission electron microscopy (TEM) analysis revealed that the silver nanoparticles have a spherical shape with an average diameter of ~6 nm. The antibacterial properties of silver nanoparticles were investigated using both Gram-positive and Gram-negative microorganisms, such as Staphylococcus aureus, Pseudomonas aeruginosa, andEscherichia coli. Klebsiella pneumoniae, and Candida albicans. Results showed a highest zone ...
ACS Sustainable Chemistry & Engineering, 2014
The objective of this study was to assess the antibacterial activity and inhibition of biofilm formation of silver nanoparticles (AgNPs) against Escherichia coli (MG1655), Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Janthinobacterium lividum. The AgNPs utilized in this study were prepared through one-pot microwave-assisted syntheses guided by principles of green chemistry. The AgNPs were synthesized in three different schemes by reducing Ag + ions (from AgNO 3) with reducing agents dextrose, arabinose, and soluble starch. Formation of AgNPs occurred in less than 15 min, and nanoparticles had diameters of 30 nm or less. Successful synthesis of AgNPs was confirmed using multiple orthogonal approaches, including UV−visible spectroscopy, fluorescence emission spectroscopy, powder X-ray diffraction, and transmission electron microscopy, while size analysis was gathered from transmission electron microscopy images and dynamic light scattering. All AgNPs prepared in this study exhibited antibacterial effects on a variety of organisms as determined by a well diffusion assay with no antibacterial effects observed in the control wells.
Green Processing and Synthesis, 2018
Silver nanoparticles (AgNPs) were synthesized usingAloe veraleaf extract as both reducing and stabilizing agentsviamicrowave irradiation method. The effects of the microwave exposure time and the amount of AgNO3solution on the mean particle size and concentration of the synthesized AgNPs solution were investigated using response surface methodology. The synthesized AgNPs were characterized by transmission electron microscopy, UV-Vis spectroscopy, and dynamic light scattering. Well-dispersed and spherically fabricated AgNPs with mean particle size (46 nm) and maximum concentration (64 ppm) and zeta potential (+15.5 mV), were obtained at optimal synthesis conditions, using 9 ml of AgNO3(1 mm) and 0.1 ml ofAloe veraextract during microwave exposure time of 360 s. The antibacterial activity of the synthesized AgNPs was tested usingEscherichia coliandStaphylococcus aureusbacteria and the obtained results indicated their significant inhibitory effects against these two Gram-negative and G...
ACS Sustainable Chemistry & Engineering, 2013
The use of silver nanoparticles (AgNPs) is gaining in popularity due to silver's antibacterial properties. Conventional methods for AgNP synthesis require dangerous chemicals and large quantities of energy (heat) and can result in formation of hazardous byproducts. This article summarizes recent activity in this general area where environmentally friendly synthetic techniques are currently being explored for the synthesis of "greener" AgNPs including the use of plant extracts, biodegradable polymers, and enzymes/bacteria and alternative energy input systems, such as microwave irradiation. Microwave heating enables efficient formation of nanostructures of uniform small sizes in shorter reaction times with reduced energy consumption; preventing agglomeration of ensuing nanoparticles is an additional attribute.
Chemical Papers, 2020
An eco-friendly and sustainable approach was developed for the synthesis of silver nanoparticles (AgNPs) of Ziziphus jujuba Mill (ZJM) fruit extract by a microwave-assisted technique. The developed method was simple, economically viable, sustainable, and eco-friendly. Concentrations of AgNO 3 and ZJM fruit extract and reaction time were optimized for the final properties of synthesized AgNPs. The prepared AgNPs were characterized by UV-visible and FTIR spectrophotometers, powder XRD, TEM, and DLS. XRD results showed a face-centered cubic structure for the prepared AgNPs. The obtained size of AgNPs was 8 ± 1 nm with spherical shape. The FTIR spectrum indicates that ZJM is a good capping agent to stabilize AgNPs. A negative zeta potentials value of − 21.2 mV indicates the stability of AgNPs. The AgNPs has good stability over a wide range of pH and ionic strength. The role and efficacy of AgNPs in the catalytic degradation of methylene blue (MB) and Congo red (CR) dye were studied, and the reduction reaction rate constants were found to be 0.033 s −1 (180 s) and 0.025 s −1 (200 s) respectively. The synthesized AgNPs showed rapid and excellent catalytic reduction of MB and CR dyes. The rapid catalytic reduction reactions followed pseudo-order kinetics. The ZJM fruit extract-capped AgNPs showed good antimicrobial activity against tested bacterial and fungal cultures.
The present investigation reports the biogenesis of silver nanoparticles (Ag NPs) using extracts of a medicinal plant Nothapodytes foetida. Total phenolic content (TPC) and ferric reducing antioxidant power (FRAP) assay were carried out for the microwaveassisted extract (MAE) of N. foetida using methanol as solvent and the conditions for extraction were optimised by response surface methodology (RSM). The effects of operating variables such as extraction time, temperature and ratio of sample to solvent were studied using central composite design (CCD). A mathematical model with a high determination coefficient (R 2 ) for TPC (0.991) and FRAP (0.995) was obtained. The optimal conditions of extraction for TPC were 48.6 C, 23.15 min and 2.04:30 (g/mL) and for FRAP 52.31 C, 12.32 min and 1.67: 30 (g/mL). Under these conditions, the experimental yields of TPC and FRAP were 2.426 mg gallic acid equivalents (GAE)/g dry powder and 14.985mg of FeSO 4 ¢7H 2 O/g of dry powder, respectively. Ag NPs were characterised using UVÀVis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The synthesised Ag NPs have also shown potent activity against the human pathogenic Staphylococcus aureus.
Green synthesis of silver nanoparticles and their antibacterial activities
Original article, 2022
Nanotechnology offers a solution to bacterial antibiotic resistance, which poses a serious threat to global health. Green synthesis of metallic nanoparticles is gaining increasing attention due to its environmental benefits. This study aimed to biosynthesize silver nanoparticles (AgNPs) by microwave irradiation through silver nitrate reduction using starch and microalgae biomass; characterize them using UV-visible spectroscopy, scanning electron microscopy-energy-dispersive X-ray microanalysis, and X-ray diffraction; and evaluate their antibacterial activity against Escherichia coli, Bacillus clausii, and Staphylococcus aureus using disk diffusion and broth dilution methods. Synthesized AgNPs showed a single peak related to surface plasmon resonance at 430 nm. Size range of spherical AgNPs was 40-150 or 90-400 nm for starch-or biomass-mediated NPs, respectively. Biomassmediated AgNPs exhibited antibacterial activity with the inhibition zones of 8, 12, and 10.5 mm against E. coli, B. clausii, and S. aureus, respectively; those starch-mediated showed inhibition of 7, 13, and 12 mm, respectively. AgNPs' minimum inhibitory concentrations were 6.25 μg•mL −1 toward both E. coli and S. aureus and 12.5 μg•mL −1 against B. clausii when using starch in biosynthesis, whereas they were 19.6 μg•mL −1 against both E. coli and S. aureus and 9.81 μg•mL −1 toward B. clausii when using biomass. Synthesized AgNPs have promising antibacterial potential.
Silver nanoparticles: green route of synthesis and antimicrobial profile
International Journal of Nanoparticles, 2015
The antimicrobial effects of silver salts have been noticed and applied since antiquity. The effectiveness of silver particles gets enhanced when used in nanosize dimensions. Silver nanoparticles are synthesised by physical, chemical and biological methods. Biological sources, such as bacteria, actinomycetes, fungi, and plants are usually favoured due to cost effectiveness, easy to scale up, easy availability of raw materials and environmental friendly nature. Moreover, plants contain various phytochemicals and compounds which may act both as reducing and stabilising agents in the synthesis of nanoparticles. The silver nanoparticles thus produced are more stable. Various plant extract derived silver nanoparticles have been scrutinised for their potent antimicrobial and anti-inflammatory properties which help them find applications in the medical domain. Moreover, they are being extensively used in agriculture, biotechnology, bio imaging, nano fabrics, electronics, bio-engineering, drug delivery, etc.