Influence of culturing media components on the growth and microbial induced calcium carbonate precipitation (MICP) activity of Lysinibacillus sphaericus (original) (raw)
Related papers
Biocatalysis and Agricultural Biotechnology , 2019
Sporosarcina pasteurii is a well-known ureolytic microbial species that proficiently induces the deposition of calcium carbonate through microbially induced carbonate precipitation (MICP) process for various biotechnological and engineering purposes. In view to resolving the concern on high-cost bacterial cultivation due to the conventional use of laboratory-grade growth medium for MICP studies, an inexpensive food-grade yeast medium was investigated in this current study for its feasibility to serve as a suitable alternative media for bacterial growth, urease activity and calcium carbonate precipitation. The effect of different media concentration and initial pH medium on biomass production and urease activity were determined. The performance of this low-cost media was also compared with eight laboratory-grade media (nutrient broth, yeast extract, tryptic soy broth, luria broth, fluid thioglycollate medium, cooked meat medium, lactose broth and marine broth). Results in this current study showed cultivation in low-cost media at 15 g L−1 (w/v) and initial pH 8.5 of the food-grade yeast media both constituted the highest biomass concentration and urease activity when supplemented with urea (4%, w/v). Comparison of the food-grade media with laboratory-grade media indicated that bacterial cultivation cost was significantly reduced to 99.80%. After the biomineralization test, X-ray diffraction (XRD) analysis was used to confirm the elemental composition of CaCO3 and polymorphs which were identified as calcite and vaterite. These findings suggest the food-grade yeast extract can serve as a potential candidate for bacterial cultivation in MICP application from the perspective of cost reduction.
Environmental harsh conditions are one of the main challenges to the survivability of bacteria during the microbially induced calcium carbonate precipitation (MICP) process. Due to the high resistivity of endospores against inhospitable conditions in comparison with vegetative cells, different sporulation methods were applied to Lysinibacillus sphaericus strain MB284 by changing the environmental conditions to investigate the growth of germinated cells. It was found that the sporulation yield was more when both carbon source starvation and the thermal shock process were applied to this bacterium. In addition, extending the sporulation time of cells into the minimal medium at 2 °C for a couple of weeks had a great impact on improving the sporulation yield. Comparing the growth rate of germinated endospores in natural conditions (pH 7 and 25 °C) and harsh conditions (pH 12, temperature of -10 to 60 °C, salinity up to 100 g/l) showed that endospores generated by thermal shock are able ...
Letters in Applied Microbiology
This study demonstrates a remarkably high level of microbial-induced calcium carbonate precipitation (MICP) using a mixed culture containing TBRC 1396 (Priestia megaterium), TBRC 8147 (Neobacillus drentensis) and ATCC 11859 (Sporosarcina pasteurii) bacterial strains. The mixed culture produced CaCO3 weights 1·4 times higher than those obtained from S. pasteurii, the gold standard for efficient MICP processes. The three strains were selected after characterization of various Bacillus spp. and related species for their ability to induce the MICP process, especially in an alkaline and high-temperature environment. Results showed that the TBRC 1396 and TBRC 8147 strains, as well as TBRC 5949 (Bacillus subtilis) and TBRC 8986 (Priestia aryabhattai) strains, could generate calcium carbonate at pH 9–12 and temperature 30–40°C, which is suitable for construction and consolidation purposes. The TBRC 8147 strain also exhibited CaCO3 precipitation at 45°C. The TBRC 8986 and TBRC 8147 strains a...
Brazilian Journal of Microbiology, 2011
The objective of this study is to investigate the efficiency of calcium carbonate bioprecipitation by Lysinibacillus sphaericus, Bacillus subtilis and Pseudomonas putida, obtained from the Coleção de Culturas do Instituto Nacional de Controle de Qualidade em Saúde (INCQS), as a first step in determining their potential to protect building materials against water uptake. Two culture media were studied: modified B4 containing calcium acetate and 295 with calcium chloride. Calcium consumption in the two media after incubation with and without the bacterial inoculum was determined by atomic absorption analysis. Modified B4 gave the best results and in this medium Pseudomonas putida INQCS 113 produced the highest calcium carbonate precipitation, followed by Lysinibacillus sphaericus INQCS 414; the lowest precipitation was produced by Bacillus subtilis INQCS 328. In this culture medium XRD analysis showed that Pseudomonas putida and Bacillus subtilis precipitated calcite and vaterite polymorphs while Lysinibacillus sphaericus produced only vaterite. The shape and size of the crystals were affected by culture medium, bacterial strain and culture conditions, static or shaken. In conclusion, of the three strains Pseudomonas putida INQCS 113 in modified B4 medium gave the best results precipitating 96% of the calcium, this strain thus has good potential for use on building materials.
Concrete is the most widely used construction material in civil engineering. But plain concrete is a brittle material and has little resistance to cracking. The cracking in concrete promotes deterioration such as the corrosion of reinforcing rebar, therefore, repair in filling the crack is often carried out. Recently, repair methods using bio-based materials associated with microbial metabolic processes leading to precipitation of calcium carbonate have been intensively studied. In this study, influencing factors on the precipitation rate depending on the constituents of bio-based material comprising yeast, glucose and calcium acetate mixed in tris buffer solution was examined for improving the rate of initial reactions. In addition, effect of temperature change on the amount of calcium carbonate precipitation was also investigated. The precipitates were identified by X-ray diffraction. It was shown that the increase of temperature lead to a change on calcium carbonate precipitation and caused the pH decrease under 7.0.
Stimulation of microbially induced calcium carbonate precipitation (MICCP) is likely to be influenced by calcium sources. In order to study such influences, we performed MICCP using Bacillus sp. CR2 in nutrient broth containing urea, supplemented with different calcium sources (calcium chloride, calcium oxide, calcium acetate and calcium nitrate). The experiment lasted 7 days, during which bacterial growth, urease activity, calcite production and pH were measured. Our results showed that calcium chloride is the better calcium source for MICCP process, since it provides higher urease activity and more calcite production. The influences of calcium sources on MICCP were further studied using Fourier transform-infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses. These analyses confirmed that the precipitate formed was CaCO 3 and composed of predominantly calcite crystals with a little amount of aragonite and vaterite crystals. The maximum yield of calcite precipitation was achievable with calcium chloride followed by calcium nitrate as a calcium source. The results of present study may be applicable to media preparation during efficient MICCP process.
Production of Bioagent for Calcium-Based Biocement
International Journal of Biotechnology for Wellness Industries, 2016
Biocements and biogrouts are developing extensively as new materials alternative to cement and toxic chemical grouts. The most popular type of biocement is a mixture of urease-producing bacteria, urea and calcium salt. Thus, development of biotechnology to produce biomass of urease-active bacteria for large-scale biocementation is an important biotechnological task. Two strains of urease-producing bacteria, Yaniella sp. VS8 and Bacillus sp. VS1 that synthesized inducible and constitutive urease, respectively, were used in the present study. It was shown that low cost biomass of urease-active bacteria can be produced from the hydrolyzed excessive activated sludge of municipal wastewater treatment plant. The biomass of Yaniella sp. VS8 grown in this medium diminished the hydraulic conductivity of sand from 4.8×10-4 m/s to 5•10-8 m/s after several biotreatments with solution of 1.5 M urea and 0.75M СаCl2.
Revista de Chimie
Lactic acid bacteria (LAB) are among the most important group of bacteria, with a critical role in food, pharmaceutical and medical industry. The fast-growing characteristics of LAB strains, their metabolic activity associated with production of many beneficial compounds and most of all, their GRAS status (Generally Recognized As Safe) recommends them as starter cultures for food biotechnology processes. During last decades, LAB strains have also an extensive prophylactic or therapeutic use as probiotics. Due to the fact that there are current limitations in the use of standard MRS media (de Man Rogosa Sharpe), which is selective especially for lactobacilli and enterococci, the aim of the present study was to optimize the growth medium composition for isolating a wide range of LAB strains with biotechnological potential and to improve the biomass accumulation. For this purpose, it has been evaluated the growth of Lactobacillus (L.) rhamnosus ATCC� 9595�, Streptococcus (S.) salivariu...