Isolation and screening endophytic bacteria producing α-glucosidase inhibitor from Sanrego plant (Lunasia amara Blanco) (original) (raw)
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2019
Alpha-glycosidase inhibitors can delay the hydrolysis of oligosaccharide and disaccharide into glucose, which can prevent or treat hyperglycemia in diabetes mellitus. The rind and flesh of Salak Pondoh fruit are known to produce α-glycosidase inhibitor compound. Endophytic bacteria that live in plant tissues potentially produce compounds such as in host plants. Exploration of endophytic bacteria from the rind and flesh of Salak Pondoh is one of the efforts to obtain isolates of bacteria producing α-glycosidase inhibitors. The objective of this study is to isolate and identify endophytic bacteria producing inhibitor α-glycosidase from rind and flesh of Salak Pondoh, and to know the activity of its α-glycosidase inhibitor. Isolation of endophytic bacteria was done by inoculating surface-sterilized fruit samples on Nutrient Agar (NA) medium. The inhibitory activity towards αglycosidase analysis was performed using the spectrophotometric method (λ = 415 nm), with p-nitro phenyl α-D-gluc...
Current Microbiology, 2013
Endophytic actinomycetes isolated from Datura stramonium L. was evaluated for its effects against in vitro a-glucosidase inhibition, antioxidant, and free radical scavenging activities. Based on microbial cultural characteristic and 16S rRNA sequencing, it was identified as Streptomyces sp. loyola UGC. The methanolic extract of endophytic actinomycetes (MeEA) shows remarkable inhibition of a-glucosidase (IC 50 730.21 ± 1.33 lg/ml), scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC 50 435.31 ± 1.79 lg/ml), hydroxyl radical (IC 50 350.21 ± 1.02 lg/ml), nitric oxide scavenging (IC 50 800.12 ± 1.05 lg/ml), superoxide anion radical (IC 50 220.31 ± 1.47 lg/ml), as well as a high and dose-dependent reducing power. The MeEA also showed a strong suppressive effect on rat liver lipid peroxidation. Antioxidants of b-carotene linoleate model system revels significantly lower than BHA. The total phenolic content of the extract was 176 mg of catechol equivalents/gram extract. Perusal of this study indicates MeEA can be used as natural resource of a-glucosidase inhibitor and antioxidants.
Endophytic actinomycetes isolated from Datura stramonium L. was evaluated for its effects against in vitro a-glucosidase inhibition, antioxidant, and free radical scavenging activities. Based on microbial cultural characteristic and 16S rRNA sequencing, it was identified as Streptomyces sp. loyola UGC. The methanolic extract of endophytic actinomycetes (MeEA) shows remarkable inhibition of a-glucosidase (IC 50 730.21 ± 1.33 lg/ml), scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC 50 435.31 ± 1.79 lg/ml), hydroxyl radical (IC 50 350.21 ± 1.02 lg/ml), nitric oxide scavenging (IC 50 800.12 ± 1.05 lg/ml), superoxide anion radical (IC 50 220.31 ± 1.47 lg/ml), as well as a high and dose-dependent reducing power. The MeEA also showed a strong suppressive effect on rat liver lipid peroxidation. Antioxidants of b-carotene linoleate model system revels significantly lower than BHA. The total phenolic content of the extract was 176 mg of catechol equivalents/gram extract. Perusal of this study indicates MeEA can be used as natural resource of a-glucosidase inhibitor and antioxidants.
International Journal of Pharmacy and Pharmaceutical Sciences
An alpha glucosidase inhibitor is one of the compounds for the treatment of diabetes. This inhibitor can retard the liberation of glucose from dietary complex carbohydrates and delay glucose absorption, resulting in reduced postprandial plasma glucose levels and suppress postprandial hyperglycaemia. The purpose of this study was to isolate and select alpha glucosidase inhibitor-producing endophytic actinomycetes from various diabetic medicinal plants. Endophytic actinomycetes were isolated from the roots, leaves and stems of diabetic medicinal plants: Alloe vera, Tinospora crispa, Phaleria macrocarpa, Curcuma aeruginosa, Centela asiatica, Xoncus arvensis, Andrographispaniculata, Caesalpinia sappan, Curcuma xanthoriza, Parcia speciosa, Gynura procumbens, Physalis peruviana andHibiscus sabdariffa. Sterilized plant sample were inoculated on the HVAgar medium containing 50 ppm cycloheximide and 30 ppm nalidixic acid and were incubated for 2-3 weeks at room temperature. Sixty-five isolat...
Alpha Glucosidase Inhibitory Activity of Selected Philippine Plants
Abstract Ethnopharmacological relevance: Antidesma bunius Spreng. (Phyllantaceae), Averrhoa bilimbi (Oxalidaceae), Biophytum sensitivum DC. (Oxalidaceae), Ceriops tagal (Perr.) C.B.Rob. (Rhizophoraceae), Kyllinga monocephala Rottb. (Cyperaceae), and Rhizophora mucronata Lam. (Rhizophoraceae) are used as remedies to control diabetes. Aim of the study: To screen the six plants for their potential α-glucosidase inhibitory activity Materials and Methods: The 80% aqueous ethanolic extracts were screened for their α-glucosidase enzyme inhibitory activity using yeast alpha glucosidase enzyme. Results: Except for A. bilimbi with IC50 value of 519.86±3.07, all manifested a significant enzyme inhibitory activity. R. mucronata manifested the highest activity with IC50 0.08±1.82 μgmL-1, followed by C. tagal IC50 0.85±1.46 μgmL-1 and B. sensitivum IC50 at 2.24±1.58 μgmL-1. Conclusion: This is the first report on the α-glucosidase inhibitory effect of the six Philippine plants; thus, partly defining the mechanism on why these medicinal plants possess antidiabetic properties. Keywords: α-Glucosidase; Antidesma bunius; Averrhoa bilimbi; Biophytum sensitivum; Ceriops tagal; Kyllinga monocephala; Rhizophora mucronata; Diabetes
α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro
Streptomyces strain isolated from the soil sediment was studied for its in vitro αglucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient glucose broth was used as the basal medium for growth and metabolites production. Ethyl acetate extract of Loyola AR1 (EA-Loyola AR1) showed 50 % α-glucosidase inhibition at the concentration of 860.50±2.68 μg/ml. Antioxidant properties such as total phenolic content of EA-Loyola AR1 was 176.83±1.17 mg of catechol equivalents/g extracts. EA-Loyola AR1 showed significant scavenging activity on 2,2diphenyl-picrylhydrazyl (50 % inhibition (IC 50 ), 750.50±1.61 μg/ml), hydroxyl (IC 50 , 690.20±2.38 μg/ml), nitric oxide (IC 50 , 850.50±1.77 μg/ml), and superoxide (IC 50 , 880.08±1.80 μg/ml) radicals, as well as reducing power. EA-Loyola AR1 showed strong suppressive effect on lipid peroxidation (IC 50 , 670.50±2.52 μg/ml). Antioxidants of βcarotene linoleate model system reveals significantly lower than butylated hydroxyanisole.
Type 2 diabetes is a disease that caused by the failure of insulin secretion by the beta cells of the pancreas and insulin resistance in peripheral levels. One therapy for diabetics is by inhibiting the activity of α-glucosidase. Lactic acid bacteria have the ability to inhibit of αglucosidase activity. The aims of this research was to isolation and screening of lactic acid bacteria from ganyong tuber (Canna Edulis) and kimpul tuber (Xanthosoma sagittifolium), which has the ability to inhibit the activity of α-glucosidase. Eightteen isolates were identified as lactic acid bacteria and all of them could inhibit the activity of α-glukosidase. The GN 8 isolate was perform the highest inhibition acivity.
International Journal of Pharmacy and Pharmaceutical Sciences, 2018
Objective: This study aims to control type 2 of diabetes mellitus by a hypoglycemic substance that extensively produced by Streptomyces bacteria. The antidiabetic action of this substance depends on prevention of starch hydrolysis and then the liberation of glucose monomers via an inhibition of α-glucosidase as one of starch hydrolyzing enzymes. Methods: The strains of marine actinomycetes were isolated on starch nitrate agar, and then qualitatively and quantitatively screened to prevent starch hydrolysis. The most potent strain was identified by classical and genetical methods. The genetic improvement of the most potent strain was carried out by using UV radiations at different exposure periods per second. The optimization of environmental conditions was studied to obtain the maximum activity of the α-glucosidase inhibitory protein, which purified and electrically separated to determine its molecular weight. Results: Among 55 marine actinomycetes, only 7 strains were found have antidiabetic activity. This activity was assayed spectrophotometrically at 400 nm, where p-nitrophenyl-α-d-glucopyranoside and acarbose were used as a substrate and a positive control respectively. The most potent strain which marked as AD-7 was identified as Streptomyces coelicolor, which exposed to the genetic improvement using UV radiations to obtain a highly activity of an inhibitory protein at 10 s of the exposure period. The activity and stability continued for 5 d at 37 °C. The maximum activity and stability of an improved inhibitory protein were obtained with optimization of environmental conditions included inoculum size (10 6 Conclusion: Alpha-glucosidase inhibitory protein as a powerful hypoglycemic agent was extracted from the filtrate of S. coelicolor. The mutant strain of the latter had been produced most active and stable inhibitory protein, which prevents the starch hydrolysis via an inhibition of αglucosidase enzyme for 5 d at 37 °C. cfu/ml/300 µl), incubation period (14 d), agitation speed (160 rpm), incubation temperature (30 °C), and pH (8.5). An inhibitor was purified and separated at 34 KDa.
α-Glucosidase inhibitory activity of selected Philippine plants
Journal of Ethnopharmacology, 2012
a b s t r a c t Ethnopharmacological relevance: Antidesma bunius Spreng. (Phyllantaceae), Averrhoa bilimbi L. (Oxalidaceae), Biophytum sensitivum (L.) DC. (Oxalidaceae), Ceriops tagal (Perr.) C.B. Rob. (Rhizophoraceae), Kyllinga monocephala Rottb. (Cyperaceae), and Rhizophora mucronata Lam.
Identification of Bacterial Endophytes Isolated From Different Medicinal Plants
Research Square (Research Square), 2023
Plants have very potential to live on earth as they supply 90% of human calorie intake, 80% of protein intake directly, and perhaps the most vital sources of medicine with a vast diversity of microorganisms. As such it's important to know those microorganisms, their kinds, the features they possess, and the signi cant compounds/metabolites they can produce. So, this study is based on identifying such microorganisms. To achieve this aim, isolation of endophytes has been done to know their biochemical activities and properties. Various identi cation procedures have been followed to get pure endophytic strains without any contamination. Surface sterilization of the plant tissue is a must in this progress, various surface sterilization techniques have been tried and nally, for 4/5 plant tissues, sodium hypochlorite and ethanol were given the best result and for 1/5 with the addition of mercuric chloride were the standardized method for surface sterilization. About 30 different bacterial endophytes have been isolated from ve kinds of medicinal plants. 4% sodium hypochlorite and 75% ethanol were found effective in sterilizing the surface of Psidium guajava, Cassia occidentalis, Calotropis procera, and Hibiscus rosa-sinensa. While Mangifera indica required an addition of 0.1% mercuric chloride. 19 strains isolated were Gram-positive, 11 Gramnegative (5 were Lactose fermenters and 6 were not), and most of which were bacilli. All isolates have shown different biochemical results, 25 showed a positive result for oxidase, and 28 gave a positive result for catalase. Most of the endophytes identi ed in this work are Bacillus spp. This identi cation is based on biochemical reactions; the exact species can be identi ed by molecular identi cation.