Optimization of nutritive media composition for xylanase production by Aspergillus awamori (original) (raw)
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Food Technology and …, 2008
Xylanase was produced by Aspergillus niger LPB 326 cultivated on lignocellulosic substrate composed by sugarcane bagasse and soybean meal in solid-state fermentation. The effects of various variables were observed and optimized by applying statistical experimental designs. The best xylanase activity was obtained in a medium containing 10 g of sugarcane bagasse and soybean meal in the ratio of 65 and 35 %, respectively, moistened to 85 % of initial water content with a nutrient salt solution composed of (in g/L): CuSO 4 0.4, KH 2 PO 4 1.5 and CoSO 4 0.0012, and incubated for 4 days at 30°C. Under these optimized conditions, a xylanase activity of 3099 IU/g of dry matter was obtained.
Fountain Journal of Natural and Applied Sciences
Xylanases are hydrolytic enzymes with wide range of applications in food processing, bleaching of pulp in paper manufacturing industry, bio-conversion of biomass wastes to fermentable sugars and enhancing nutrient digestibility in animal feeds. The optimization of growth conditions and evaluation of an appropriate substrate as carbon source among cassava peels, corn cobs, wheat bran and rice husk on xylanase production by novel strain of Aspergillus tubingensis under Solid State Fermentation (SSF) was investigated. The fungal isolate was identified based on ribosomal RNA gene and ITS gene sequencing analysis as Aspergillus tubingensis. Results showed that Corn cobs had the highest xylanase production among the four substrates. Corn cobs recorded the highest value of xylanase production at pH of 6.0 (107.97 U/g), after incubation period of 72 hour (111.23 U/g), at temperature of 30oC (44.26 U/g) and at ratio 1:3 (45.68 U/g). The optimum growth conditions for xylanase production by As...
Food Technology and Biotechnology, 2011
The Plackett-Burman screening design (PBSD) and Central composite rotatable design (CCRD) were used to optimize the fermentation parameters for enhanced xylanase production from Aspergillus flavus DFR-6 in submerged cultivation. The PBSD demonstrated that the positive main effects of yeast extract, wheat bran, Tween 80 and NaNO 3 were significant at 10 % level of significance. The interactive effects of these factors were deduced using CCRD and finally, the production medium was optimized using Design-Expert software. The optimized medium conditions with 97.2 % desirability and with respect to`minimum organic nitrogen source' were (in g/L): NaNO 3 4.09, K 2 HPO 4 0.5, MgSO 4 •7H 2 O 0.25, KCl 0.25, FeSO 4 0.005, wheat bran 24.99, yeast extract 10 and Tween 80 0.21 mL/L, pH=5.0. The enzyme was produced in 50 mL of medium working volume using 500-mL conical flask at 35°C with 1 % (by volume) of inoculum size. The production titre of xylanase under aforementioned conditions was 31.4 U/mL after 6 days of static fermentation which is approx. fourfold higher than obtained from the unoptimized medium.
Optimization of Xylanase production from Aspergillus niger by Solid state fermentation.docx
Aspergillus niger is one of the most important filamentous fungi that used in the fermentation industry. Aspergillus niger isolate was cultured on potato-dextrose agar (PDA) for activation, and the optimum conditions for xylanase production from this local isolate were studied by solid state fermentation, using a medium composed of wheat bran moisten with corn steep liquor at ratio 1:0.5 (v:w) at initial pH 5.5, inoculated with 1.6 × 106 spores/ml, and incubated at 30Cᵒ for 5 days.
2016
The enzyme super-secreter Aspergillus niger KR-3 was isolated from putrefied soil and grown for optimized production of xylanase in Solid State Fermentation (SSF). Xylanase production was carried out by growing the strain for 5 days at 40 o C on lignocellulosic base material such as wheat bran moistened with mineral salt solution. The extracellular xylanase was purified with 42.16% recovery and 5.3-fold purification using conventional chromatography. The partially purified enzyme followed Michaelis-Menten behavior with Km and Vmax values 0.3% and 5UmL -1 , respectively for oat spelts xylan. Xylanase was found active over a wide range of pH with two apparent optima at 6 and 8. The optimum temperature for enzyme active was 50 o C, and it was thermostable up to 45 o C for 1 h. The catalytic activity of enzyme was increased by 3.25-fold in the presence of Mn 2+ . In the presence of MnCl2, enzyme exhibited broader pH profile with a shift in the minor pH optima from 8 to 9. With MnCl2 in ...
Xylanase Activity of Aspergillus Niger at Different Ecological Conditions
Plant Archives, 2021
Maximization of xylanase activity at different media, temperature, pH and salt concentration has been presented in this paper. YpSs, Czapek dox and Malt extract medium were taken for evaluation of optimum growth and activity. Amongst all tested media, YpSs showed the highest growth. Three different natural carbon substitute i.e., wheat husk, rice husk and sugarcane baggase were used for xylanase activity. Maximum enzyme activity was observed in test fungus at rice husk. Production and maximum xylanase activity at rice husk has been observed at different temperatures, pH and Salt concentrations. The highest xylanase activity has been observed on day 5 at temperature 32° C, pH 6.5 and salt concentration of 2%.
Present investigation deals with the studies on the cultural conditions for enhanced biosynthesis of xylanase by a chemically mutated strain of Aspergillus niger GCBCX-20. The effects of time course, incubation temperature, medium pH and volume of fermentation medium on the production of xylanase were studied. The maximum xylanase production (250 U mL-1) was observed at an incubation temperature of 30 o C after 48 h of incubation period. The optimum initial pH of the medium was 5.0 because maximum production of xylanase (260 U mL-1) was observed at this pH. The production of enzyme was found to be maximum (265 U/ml) when the volume of fermentation medium was kept as 25 mL/250 mL conical flasks. All the fermentation batches were carried out in triplicates under submerged conditions.
Production of xylanase from Aspergillus terreus under liquid state fermentation condition
2012
Various parameters like pH, temperature, substrate concentration, incubation period and yeast extract concentration have been used in estimating the enzyme production by Aspergillus terreus. Incubation period was optimized on 8 th day of fungal inoculation at 6.0 pH, 40 0 C temperature and 2% wheat bran was used as substrate for optimum production of xylanase. Total 5 mg/ml phosphate buffer and birch wood xylan were taken as substrate at a pH of 6.5 and the temperature of 55 0 C for xylanase activity. Thus xylanase preparation by this fungus was optimum at 40 0 C and this enzyme was found to be thermophilic in nature.
Bioscience Journal, 2016
This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 o C). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g-1 of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.